FHL2 (2A4) Monoclonal Antibody

$Lane 1: Rat Heart; Lane 2: Mouse Heart; Probed with VFHL2 (2A4) Monoclonal Antibody (bsm-54368R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Rat Heart; Lane 2: Mouse Heart; Probed with VFHL2 (2A4) Monoclonal Antibody (bsm-54368R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with FHL2 (2A4) Monoclonal Antibody (bsm-54368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with FHL2 (2A4) Monoclonal Antibody (bsm-54368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Fetal Heart tissue incubated with FHL2 (2A4) Monoclonal Antibody (bsm-54368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Fetal Heart tissue incubated with FHL2 (2A4) Monoclonal Antibody (bsm-54368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated with FHL2 (2A4) Monoclonal Antibody (bsm-54368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated with FHL2 (2A4) Monoclonal Antibody (bsm-54368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54368R
Product Name	FHL2 (2A4) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within C-terminal human FHL2.
Clonality	Monoclonal
Clone #	2A4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	2274
Swiss Prot	Q14192
Subcellular location	Nucleus
Synonyms	Four and a half LIM domains protein 2; LIM domain protein DRAL; Skeletal muscle LIM-protein 3; FHL-2; SLIM-3; DRAL; SLIM3
Background	May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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