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SFRP4 (2F1) Monoclonal Antibody

Lane 1: Mouse Testis; Lane 2: A549 Cells; Lane 3: HepG2 Cells; Lane 4: SH-SY5Y Cells; Probed with SFRP4 (2F1) Monoclonal Antibody (bsm-54453R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Testis; Lane 2: A549 Cells; Lane 3: HepG2 Cells; Lane 4: SH-SY5Y Cells; Probed with SFRP4 (2F1) Monoclonal Antibody (bsm-54453R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of A549 cells with SFRP4 (2F1) Monoclonal Antibody (bsm-54453R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).
Flow cytometric analysis of A549 cells with SFRP4 (2F1) Monoclonal Antibody (bsm-54453R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-54453R
Product Name SFRP4 (2F1) Monoclonal Antibody
Applications WB, FCM, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Full length recombinant protein of human SFRP4.
Clonality Monoclonal
Clone # 2F1
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 6424
Swiss Prot Q6FHJ7
Subcellular location Secreted
Synonyms Secreted frizzled-related protein 4; Frizzled protein human endometrium; sFRP-4; FrpHE; SFRP4; FRPHE
Background Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(ICC) 1:50-200

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