SAM68 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAM68 Monoclonal Antibody, Unconjugated (bsm-54647R) at 1:1000 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAM68 Monoclonal Antibody, Unconjugated (bsm-54647R) at 1:1000 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAM68 Monoclonal Antibody, Unconjugated (bsm-54647R) at 1:1000 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAM68 Monoclonal Antibody, Unconjugated (bsm-54647R) at 1:1000 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAM68 Monoclonal Antibody, Unconjugated (bsm-54647R) at 1:1000 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAM68 Monoclonal Antibody, Unconjugated (bsm-54647R) at 1:1000 for 30 minutes at room temperature, DAB staining.

$A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (SAM68) Monoclonal Antibody, Unconjugated (bsm-54647R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.$

A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (SAM68) Monoclonal Antibody, Unconjugated (bsm-54647R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54647R
Product Name	SAM68 Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within C terminal human SAM68.
Clonality	Monoclonal
Clone #	4B4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	10657
Swiss Prot	Q07666
Subcellular location	Cytoplasm, Nucleus, Membrane
Synonyms	FLJ34027 antibody, GAP associated tyrosine phosphoprotein p62 antibody, GAP-associated tyrosine phosphoprotein p62 antibody, KH domain containing RNA binding signal transduction associated 1 antibody, KH domain-containing antibody, KHDR1_HUMAN antibody, Khdrbs1 antibody, p21 Ras GTPase activating protein associated p62 antibody, p21 Ras GTPase-activating protein-associated p62 antibody, p62 antibody, p68 antibody, RNA-binding antibody, Sam68 antibody, signal transduction-associated protein 1 antibody, Src associated in mitosis 68 kDa protein antibody, Src-associated in mitosis 68 kDa protein antibody
Background	Sam 68 is a protein that is phosphorylated on tyrosine and functions as a substrate for Src family tyrosine kinases during mitosis. Sam 68 also associates with several SH2 and SH3 domain-containing signaling proteins, such as GRB2 and PLC ?. Originally cloned as Ras GAP-associated p62, further investigations have shown that Sam 68 and Ras GAP-associated p62 are not antigenically related, nor are they encoded by the same gene. Like Sam 68, the Sam 68-like mammalian proteins, SLM-1 and SLM-2, demonstrate RNA binding activity. Also like Sam 68, SLM-1 is tyrosine phosphorylated and functions as an adapter protein for signaling molecules, including GRB2, PLC ?, Fyn and Ras GAP. SLM-2 is not tyrosine phosphorylated, nor does it appear to associate with GRB2, PLC ?, Fyn or Ras GAP, indicating that SLM-2 may not be an adapter protein for these proteins.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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