Plastin L Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plastin L Monoclonal Antibody, Unconjugated (bsm-54671R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plastin L Monoclonal Antibody, Unconjugated (bsm-54671R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plastin L Monoclonal Antibody, Unconjugated (bsm-54671R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plastin L Monoclonal Antibody, Unconjugated (bsm-54671R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plastin L Monoclonal Antibody, Unconjugated (bsm-54671R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plastin L Monoclonal Antibody, Unconjugated (bsm-54671R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plastin L Monoclonal Antibody, Unconjugated (bsm-54671R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plastin L Monoclonal Antibody, Unconjugated (bsm-54671R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plastin L Monoclonal Antibody, Unconjugated (bsm-54671R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plastin L Monoclonal Antibody, Unconjugated (bsm-54671R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Flow cytometric analysis of Plastin L was done on THP-1 cells. The cells were fixed, permeabilized and stained with Plastin L antibody at 1\/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black$

Flow cytometric analysis of Plastin L was done on THP-1 cells. The cells were fixed, permeabilized and stained with Plastin L antibody at 1\/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human

Overview
Catalog #	bsm-54671R
Product Name	Plastin L Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within C terminal human Plastin L.
Clonality	Monoclonal
Clone #	12G3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Swiss Prot	P13796
Subcellular location	Cytoskeleton, Plasma membrane
Synonyms	CP64 antibody, L plastin antibody, L-plastin antibody, Larval cuticle protein 1 antibody, LC64P antibody, LCP-1 antibody, LCP1 antibody, LPL antibody, Lplastin antibody, Lymphocyte cytosolic protein 1 antibody, Plastin 2 antibody, Plastin-2 antibody, PLS2 antibody, PLSL_HUMAN antibody
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC

Applications

Reactivity

Search our catalog of hundreds of thousands of products