NRG3 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NRG3 Monoclonal Antibody, Unconjugated (bsm-54680R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NRG3 Monoclonal Antibody, Unconjugated (bsm-54680R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NRG3 Monoclonal Antibody, Unconjugated (bsm-54680R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NRG3 Monoclonal Antibody, Unconjugated (bsm-54680R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Flow cytometric analysis of NRG3 was done on F9 cells. The cells were fixed, permeabilized and stained with Carcino Embryonic Antigen CEA antibody at 1\/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor\u2122 488-conjugated goat anti-rabbit IgG Secondary antibody at 1\/500 dilution for 30 minutes.$

Flow cytometric analysis of NRG3 was done on F9 cells. The cells were fixed, permeabilized and stained with Carcino Embryonic Antigen CEA antibody at 1\/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor\u2122 488-conjugated goat anti-rabbit IgG Secondary antibody at 1\/500 dilution for 30 minutes.

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54680R
Product Name	NRG3 Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within C-terminal human NRG3.
Clonality	Monoclonal
Clone #	9D13
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	10718
Swiss Prot	P56975
Subcellular location	Secreted, Membrane
Synonyms	HRG3 antibody, Neuregulin 3 antibody, Neuregulin 3 like polypeptide antibody, Neuregulin-3 antibody, Neuregulin3 antibody, NRG-3 antibody, Nrg3 antibody, NRG3_HUMAN antibody, Pro neuregulin 3 antibody, pro NRG3 antibody, Pro-NRG3 antibody
Background	Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC

Applications

Reactivity

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