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20uL recombinant mAbs



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Lane 1: Rat Heart; Probed with PDK1 (2F9) Monoclonal Antibody (bsm-54037R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with PDK1 (2F9) Monoclonal Antibody (bsm-54037R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PDK1 (2F9) Monoclonal Antibody (bsm-54037R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with PDK1 (2F9) Monoclonal Antibody (bsm-54037R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54037r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated(bsm-54036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded HumanEsophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated(bsm-54036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated(bsm-54036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated(bsm-54036R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54036r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LDLR Monoclonal Antibody, Unconjugated(bsm-52372R) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LDLR Monoclonal Antibody, Unconjugated(bsm-52372R) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LDLR Monoclonal Antibody, Unconjugated(bsm-52372R) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LDLR Monoclonal Antibody, Unconjugated(bsm-52372R) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52372r-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

MCF7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GSK3 beta(Ser 9) (7A4) Monoclonal Antibody(bsm-52160R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GSK3 beta(Ser 9) (7A4) Monoclonal Antibody(bsm-52160R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSK3 beta(Ser 9) (7A4) Monoclonal Antibody, Unconjugated (bsm-52160R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSK3 beta(Ser 9) (7A4) Monoclonal Antibody, Unconjugated (bsm-52160R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52160r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated(bsm-54764R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated(bsm-54764R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated(bsm-54764R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated(bsm-54764R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54764r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61745r-pe-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: PE

Catalog #1: bsm-61745r-fitc-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: FITC

Catalog #1: bsm-61745r-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: Cy5.5

Catalog #1: bsm-61745r-bf750-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61745r-cy3-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: Cy3

Catalog #1: bsm-61745r-cy7-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: Cy7

Catalog #1: bsm-61745r-bf680-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61745r-bf405-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61745r-cy5-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: Cy5

Catalog #1: bsm-61745r-bf555-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61745r-bf647-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61745r-bf594-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61745r-bf488-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61745r-bf350-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61745r-apc-cy7-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: APC-Cy7

Catalog #1: bsm-61745r-apc-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-61745r-apc-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: APC

Catalog #1: bsm-61743r-pe-Cy5.5-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-61743r-rbitc-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-61743r-percp-cy7-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-61743r-pe-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-61743r-pe-cy5-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-61743r-percp-Cy5.5-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-61743r-percp-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-61743r-pe-cy7-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-61743r-bf647-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61743r-fitc-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-61743r-cy7-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-61743r-cy5-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-61743r-cy3-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-61743r-bf750-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61743r-Cy5.5-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-61743r-bf680-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61743r-bf405-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61743r-bf555-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61742r-percp-cy7-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-61743r-bf350-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61743r-apc-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-61742r-rbitc-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-61743r-bf594-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61743r-bf488-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61743r-apc-cy7-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-61743r-apc-Cy5.5-100ul

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5