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Secondary Antibodies

Our Secondary Antibodies for reliable detection in Western blot, IHC, ICC, IF, flow cytometry, ELISA, and related immunoassays. Our collection includes species-specific secondary antibodies against rabbit, mouse, goat, rat, and other primary antibody hosts, with HRP, biotin, fluorophore, and unconjugated formats available to support a wide range of research workflows.



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Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.

Catalog #1: bs-0295G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.

Catalog #1: bs-0296G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0293G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-0368G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0295G

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0295G-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0295D-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0296G

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0296G-BF488

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0294D-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (RAT brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-Bio 1:2000) instructionsand DAB staining.

Catalog #1: bs-0295G-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: Biotin

Lane 1: Serum (Mouse) at 30 ug\r\nLane 2: Plasma (Mouse) at 30 ug\r\nLane 3: Liver (Mouse) Tissue Lysate at 40 ug\r\nLane 4: Cerebrum (Mouse) Tissue Lysate at 40 ug\r\nLane 5: Spleen (Mouse) Tissue Lysate at 40 ug\r\nPrimary: Mouse IgA (bs-0774R-HRP) at 1\/1000 dilution\r\nSecondary: Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 55 kD\r\nObserved band size: 62 kD
Lane 1: Serum (Mouse) at 40 ug\r\nLane 2: Small intestine (Mouse) Lysate at 40 ug\r\nLane 3: Lung (Mouse) Lysate at 40 ug\r\nPrimary: Mouse IgA (bs-0774R) at 1\/1000 dilution\r\nSecondary: Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 55 kD\r\nObserved band size: 60 kD

Catalog #1: bs-0774r

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0293R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-0294R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Rat IgG Polyclonal Antibody, Unconjugated (bs-0293R) at 1:600, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Rat IgG Polyclonal Antibody, FITC conjugated (bs-0293R-FITC) at 1:200, followed by DAPI staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Rat IgG Polyclonal Antibody, FITC conjugated (bs-0293R-FITC) at 1:200

Catalog #1: bs-0293R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-0295G-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: FITC

Catalog #1: bs-0296R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0432R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Unconjugated

Catalog #1: bs-0295G-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0295G-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 5

Catalog #1: bs-0295D

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0310G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Chicken

Conjugate: HRP

Catalog #1: bs-0295M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-0294D-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: Biotin

Catalog #1: bs-0403R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0345G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0295G-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0358G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Guinea Pig

Conjugate: HRP

Catalog #1: bs-0297G-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0296R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0296G-BF647

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0295G-PE

Applications: WB, FCM

Reactivity: Rabbit

Conjugate: Phycoerythrin

Catalog #1: bs-0294D

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Goat

Conjugate: Unconjugated

Catalog #1: bs-0336R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Monkey

Conjugate: HRP

Catalog #1: bs-0330R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0432R-HRP

Applications: WB, IHC-P

Reactivity: Chicken

Conjugate: HRP

Catalog #1: bs-0358D-BF750

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Guinea Pig

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0297G

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0295M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0295D-BF488

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0368G

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0364R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0330R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0303R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Dog

Conjugate: HRP

Catalog #1: bs-0296G-BF594

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0375R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Guinea Pig

Conjugate: HRP

Catalog #1: bs-0368R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0335R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Monkey

Conjugate: HRP