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Secondary Antibodies

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Catalog #1: bs-60296G

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-60296G-BF350

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 350

Catalog #1: bs-60296G-BF405

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 405

Catalog #1: bs-60296G-BF488

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 488

Catalog #1: bs-60296G-BF555

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 555

Catalog #1: bs-60296G-BF594

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 594

Catalog #1: bs-60296G-BF647

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 647

Catalog #1: bs-60296G-BF680

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 680

Catalog #1: bs-60296G-BF750

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 750

Catalog #1: bs-60296G-APC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Allophycocyanin

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:500) instructionsand DAB staining.

Catalog #1: bs-60296G-Biotin

Applications: IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-60296G-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cyanine 3

Catalog #1: bs-60296G-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cyanine 5

Catalog #1: bs-60296G-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cyanine 5.5

Catalog #1: bs-60296G-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cyanine 7

Catalog #1: bs-60296G-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4\u00b0C\uff0c An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37\u2103\uff0cDAB was used as the chromogen\u3002
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C\uff0c An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37\u2103\uff0cDAB was used as the chromogen

Catalog #1: bs-60296G-HRP

Applications: IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-60296G-PerCP

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Peridinin Chlorophyll Protein

Catalog #1: bs-60296G-PE

Applications: FCM

Reactivity:

Conjugate: Phycoerythrin

Catalog #1: bs-60296G-RBITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Rhodamine B Isothiocyanate

Lane 1: Serum (Mouse) at 30 ug\r\nLane 2: Plasma (Mouse) at 30 ug\r\nLane 3: Liver (Mouse) Tissue Lysate at 40 ug\r\nLane 4: Cerebrum (Mouse) Tissue Lysate at 40 ug\r\nLane 5: Spleen (Mouse) Tissue Lysate at 40 ug\r\nPrimary: Mouse IgA (bs-0774R-HRP) at 1\/1000 dilution\r\nSecondary: Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 55 kD\r\nObserved band size: 62 kD
Lane 1: Serum (Mouse) at 40 ug\r\nLane 2: Small intestine (Mouse) Lysate at 40 ug\r\nLane 3: Lung (Mouse) Lysate at 40 ug\r\nPrimary: Mouse IgA (bs-0774R) at 1\/1000 dilution\r\nSecondary: Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 55 kD\r\nObserved band size: 60 kD

Catalog #1: bs-0774R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0774R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-0774R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-0774R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0432R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Unconjugated

Catalog #1: bs-0432R-BF350

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0432R-BF405

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0432R-BF488

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0432R-BF555

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0432R-BF594

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0432R-BF647

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0432R-BF680

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0432R-BF750

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0432R-APC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Allophycocyanin

Catalog #1: bs-0432R-Biotin

Applications: WB, IHC-P

Reactivity: Chicken

Conjugate: Biotin

Catalog #1: bs-0432R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Cyanine 3

Catalog #1: bs-0432R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Cyanine 5

Catalog #1: bs-0432R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Cyanine 5.5

Catalog #1: bs-0432R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Cyanine 7

Catalog #1: bs-0432R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: FITC

Catalog #1: bs-0432R-HRP

Applications: WB, IHC-P

Reactivity: Chicken

Conjugate: HRP

Catalog #1: bs-0432R-PerCP

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Peridinin Chlorophyll Protein

Catalog #1: bs-0432R-PE

Applications: FCM

Reactivity: Chicken

Conjugate: Phycoerythrin

Catalog #1: bs-0432R-RBITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Rhodamine B Isothiocyanate