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Secondary Antibodies

Secondary Antibodies

Our Secondary Antibodies support reliable detection in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and related immunoassays. Our collection includes species-specific secondary antibodies against rabbit, mouse, goat, rat, and other primary antibody hosts, with HRP, biotin, fluorophore, and unconjugated formats available to support a wide range of research workflows.



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Catalog #1: bs-60296g-bf555-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 555

Catalog #1: bs-60296g-bf350-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 350

Catalog #1: bs-60296g-BF750-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 750

Catalog #1: bs-60296g-bf680-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 680

Catalog #1: bs-60296g-bf647-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 647

Catalog #1: bs-60296g-bf594-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 594

Catalog #1: bs-60296g-fitc-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-60296g-bf488-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 488

Catalog #1: bs-60296g-bf405-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 405

Catalog #1: bs-60296g-cy7-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-60296g-pe-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: PE

Catalog #1: bs-60296g-cy3-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-60296g-cy5.5-200ul

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy5.5

Catalog #1: bs-0432R-RBITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Rhodamine B Isothiocyanate

Catalog #1: bs-0432R-PerCP

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Peridinin Chlorophyll Protein

Catalog #1: bs-0432R-BF405

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0432R-APC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Allophycocyanin

Catalog #1: bs-0432R-BF750

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0432R-BF647

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0432R-BF680

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0432R-BF488

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0432R-BF594

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0432R-BF555

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0432R-BF350

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0432R-PE

Applications: FCM

Reactivity: Chicken

Conjugate: Phycoerythrin

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:500) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-60296G-Biotin 1:500) instructionsand DAB staining.

Catalog #1: bs-60296g-biotin-200ul

Applications: IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4\u00b0C\uff0c An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37\u2103\uff0cDAB was used as the chromogen\u3002
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C\uff0c An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37\u2103\uff0cDAB was used as the chromogen

Catalog #1: bs-60296g-hrp-200ul

Applications: IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0774R-FITC

Applications: WB

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-0774R-Biotin

Applications: WB

Reactivity: Mouse

Conjugate: Biotin