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20uL recombinant mAbs



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Catalog #1: bsm-62931r-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-62931r-bf750

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-62931r-cy5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-62931r-bf555

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-62931r-bf647

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-62931r-bf680

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-62930r-apc-cy7

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-62931r-bf594

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-62931r-apc-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-62931r-bf488

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-62931r-apc-cy7

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

25 ug total protein per lane of various lysates (see on figure) probed with PIAS1\/2 monoclonal antibody, unconjugated (bsm-62931R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PIAS1\/2 antibody (bsm-62931R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PIAS1\/2) monoclonal Antibody, unconjugated (bsm-62931R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIAS1\/2 Monoclonal Antibody, Unconjugated (bsm-62931R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62931r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62930r-percp-cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-62931r-bf405

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-62931r-apc

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-62930r-rbitc

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-62931r-bf350

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-62930r-percp

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-62930r-bf750

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-62930r-bf555

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-62930r-fitc

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-62930r-cy3

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-62930r-bf680

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-62930r-Cy5.5

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-62930r-percp-Cy5.5

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-62930r-bf488

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-62930r-pe

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-62930r-bf647

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-62930r-cy7

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-62930r-bf594

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-62930r-cy5

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-62930r-apc

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-62930r-bf350

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-62930r-bf405

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-62930r-apc-Cy5.5

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-62930r

Applications: WB, IP, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62928r-rbitc

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: RBITC

Catalog #1: bsm-62928r-percp-Cy5.5

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-62928r-percp

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bsm-62928r-percp-cy7

Applications: WB

Reactivity: Human, Mouse

Conjugate: PerCP-Cy7

Catalog #1: bsm-62928r-pe

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-62928r-cy7

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-62928r-cy5

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-62928r-Cy5.5

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-62928r-fitc

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-62928r-bf594

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-62928r-cy3

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-62928r-bf750

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750