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20uL recombinant mAbs



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25 ug total protein per lane of various lysates (see on figure) probed with A-RAF monoclonal antibody, unconjugated (bsm-52015R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with A-RAF Monoclonal Antibody, Unconjugated (bsm-52015R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52015r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with PPAR gamma monoclonal antibody, unconjugated (bsm-52220R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature.Primary Antibody (green):Rabbit Anti-PPAR gamma antibody (bs-52220R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PPAR gamma) monoclonal Antibody, unconjugated (bsm-52220R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-52220r-TR

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PI3-kinase p110 subunit beta) monoclonal Antibody, unconjugated (bsm-52218R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated(bsm-52218R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated(bsm-52218R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated(bsm-52218R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-52218r-TR

Applications: WB, IHC-P, IHC-F, IF(ICC), IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human skin lysates probed with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody .
Paraformaldehyde-fixed and paraffin-embedded Human skin cancer tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human esophagus tissue incubated with Cytokeratin 2e (2F7) Monoclonal Antibody (bsm-52061R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52061r

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TrkB(Y817) (2A1) Monoclonal Antibody, Unconjugated (bsm-52213R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Spinal cord tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates probed with Phospho-TrkB (Tyr817) Monoclonal Antibody, Unconjugated (bsm-52213R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-TrkB (Tyr817) Monoclonal Antibody, Unconjugated (bsm-52213R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-TrkB (Tyr817) Monoclonal Antibody, Unconjugated (bsm-52213R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52213r-TR

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HDAC1Monoclonal Antibody, Unconjugated(bsm-52080R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HDAC1Monoclonal Antibody, Unconjugated(bsm-52080R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HDAC1Monoclonal Antibody, Unconjugated(bsm-52080R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HDAC1Monoclonal Antibody, Unconjugated(bsm-52080R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52080r-TR

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52009r-cy5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-61501r-fitc-100ul

Applications: IF

Reactivity: Human

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated(bsm-54764R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated(bsm-54764R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated(bsm-54764R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PLIN2 Monoclonal Antibody, Unconjugated(bsm-54764R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54764r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61745r-pe-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: PE

Catalog #1: bsm-61745r-hrp-100ul

Applications: WB

Reactivity: Rat

Conjugate: HRP

Catalog #1: bsm-61745r-fitc-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: FITC

Catalog #1: bsm-61745r-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: Cy5.5

Catalog #1: bsm-61745r-bf750-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61745r-cy3-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: Cy3

Catalog #1: bsm-61745r-cy7-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: Cy7

Catalog #1: bsm-61745r-bf680-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61745r-biotin-100ul

Applications: WB

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bsm-61745r-bf405-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61745r-cy5-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: Cy5

Catalog #1: bsm-61745r-bf555-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61745r-bf647-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61745r-bf594-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61745r-bf488-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61745r-bf350-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61745r-apc-cy7-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: APC-Cy7

Catalog #1: bsm-61744r-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-61745r-apc-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-61744r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-61744r-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-61745r-apc-100ul

Applications: WB, FCM

Reactivity: Rat

Conjugate: APC

Catalog #1: bsm-61744r-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-61744r-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-61744r-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-61744r-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-61744r-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-61744r-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-61744r-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-61744r-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-61744r-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-61744r-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-61744r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-61744r-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-61744r-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61744r-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61744r-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61744r-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61744r-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-555