CD43 Recombinant Antibody

$Lane 1: Human U937 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human Jurkat cell lysates probed with CD43 Monoclonal Antibody, Unconjugated (bsm-60237R) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human U937 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human Jurkat cell lysates probed with CD43 Monoclonal Antibody, Unconjugated (bsm-60237R) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

The U937 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD43 antibody (bsm-60237R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

Reactivity

Human

Overview
Catalog #	bsm-60237r
Product Name	CD43 Recombinant Antibody
Applications	WB FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Clonality	Recombinant
Clone #	H6G11
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P16150
Subcellular location	Cell membrane
Synonyms	CD43; GALGP; GPL115; LEU-22; LSN; A630014B01Rik; Ly-48; Ly48; LEUK_HUMAN; SPN; Galactoglycoprotein (GALGP); Leukocyte sialoglycoprotein; Sialophorin; LEUK_MOUSE; B-cell differentiation antigen LP-3; Lymphocyte antigen 48 (Ly-48); sialophorin (gpL115, leukosialin, CD43); leukosialin
Application Dilution
WB	1:2000-10000
FCM	FCM1:50-100