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Antigen: bs-9861P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
U2OS cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CMD1G Antibody\uff08bs-9861R\uff09 at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Molt4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103 and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Titin Polyclonal Antibody(bs-9861R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Muscle cell lysates; Lane 2: Rat Muscle cell lysates probed with Titin Polyclonal Antibody, Unconjugated (bs-9861R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9861R

Applications: ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4886R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

L1 mouse intestine lysates probed with Anti TNFR1\/TNF Receptor I Polyclonal Antibody, Unconjugated (bs-2941R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 40kD\/26kD. Observed band size:40kD\/26kD.\\n
TM4 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNF Antibody\uff08bs-2941R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Lane 1: Rat Cerebrum lysates probed with TNF Receptor I Polyclonal Antibody, Unconjugated (bs-2941R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNF Receptor I Polyclonal Antibody(bs-2941R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2941R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GADD153 Polyclonal Antibody, Unconjugated (bs-1361R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DDIT3) polyclonal Antibody, Unconjugated (bs-1361R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1361R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human A549 lysates probed with Pan-Actin Polyclonal Antibody, Unconjugated (bs-10196R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
HeLa cells probed with Pan-Actin Polyclonal Antibody, unconjugated (bs-10196R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Lane 1: Rat Liver lysates; Lane 2: Rat Urinary bladder lysates; Lane 3: Mouse Stomach lysates; Lane 4: A549 cell lysates probed with Pan-Actin Polyclonal Antibody, Unconjugated (bs-10196R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A549 cell lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3: Hela cell lysates; Lane 4: A431 cell lysates ; Lane 5: HepG2 cell lysates;Lane 6: Mouse Stomach lysates; Lane 7: Mouse Lung lysates; Lane 8: Mouse Skeletal muscle (-) lysates probed with Pan-Actin Polyclonal Antibody, Unconjugated (bs-10196R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10196R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Tau protein Polyclonal Antibody (bs-0157R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tTau protein Polyclonal Antibody, Unconjugated (bs-0157R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml) was used to stain the cell nuclei.
Lane 1: Brain(Mouse) lysates; Lane 2: Brain(Rat) lysates; Lane 3: U251 lysates probed with Tau protein Polyclonal Antibody, Unconjugated (bs-0157) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Human MCF-7 Cells fixed with 70% methanol (Overnight at 4\u2103) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells were then probed with rabbit anti-Tau Polyclonal Antibody, Unconjugated (bs-0157R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).

Catalog #1: bs-0157R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TTR Prealbumin beta Polyclonal Antibody, Unconjugated (bs-0152R) at 1:400 overnight at 4\u00b0C, DAB staining.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Prealbumin Antibody(bs-0152R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (TTR\/Prealbumin) polyclonal Antibody, Unconjugated (bs-0152R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0152R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TRP12 Polyclonal Antibody, Unconjugated (bs-6425R) at 1:400 overnight at 4\u00b0C, DAB staining.
NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (TRP12\/TRPV4) polyclonal Antibody, Unconjugated (bs-6425R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-6425r

Applications: FCM, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Bovine

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-AQP7 Polyclonal Antibody, Unconjugated (bs-2506R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-AQP7 Polyclonal Antibody, Unconjugated (bs-2506R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-AQP7 Polyclonal Antibody, Unconjugated (bs-2506R) at 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
A549 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nAQP7 Antibody\uff08bs-2506R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Catalog #1: bs-2506R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial tissue labeled with Anti Estrogen Receptor alpha Polyclonal Antibody (bs-2098R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
MCF-7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Estrogen Receptor alpha Polyclonal Antibody(bs-2098R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Estrogen Receptor alpha Polyclonal Antibody, Unconjugated (bs-2098R) at 1:200 overnight at 4\u00b0C, DAB staining.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-2098R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2098R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Leptin Receptor Polyclonal Antibody(bs-0109R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0109R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-ACE1 Polyclonal Antibody, Unconjugated (bs-0439R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse lung lysates Lane 2: mouse liver lysates, Lane 3: mouse adrenal lysates probed with Rabbit Rabbit Anti-ACE Polyclonal Antibody, Unconjugated (bs-0439R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0439R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

This image was generously provided by Juanli Fu, at Southwest University in Chong Qing, China. 4% Paraformaldehyde fixed PC12 cells stained with Rabbit Anti- TLR4 Polyclonal Antibody (bs-20594R) at 1:300 for 3 hours at 4\u00b0C, followed by Alexa Fluor 488-conjugated secondary antibody for an additional hour.
Lane 1: K562 lysates; Lane 2: HL-60 lysates; Lane 3: RAW264.7 lysates probed with TLR4 Polyclonal Antibody, Unconjugated (bs-20594R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates; Lane 2: HUVEC cell lysates; Lane 3: U-2OS cell lysates; Lane 4: Mouse Lymph node lysates ; Lane 5: Mouse Raw264.7 cell lysates probed with TLR4 Polyclonal Antibody, Unconjugated (bs-20594R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Placenta lysates; Lane 2: Rat Lymph node lysates; Lane 3: Mouse BV2-lps cell lysates; Lane 4: Human HL60 cell lysates ; Lane 5: Human K562 cell lysates ; Lane 6: Human A549 cell lysates probed with TLR4 Polyclonal Antibody, Unconjugated (bs-20594R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20594R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Unconjugated

Catalog #1: bs-4819R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SGPL1) Polyclonal Antibody, Unconjugated (bs-4188R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SGPL1) Polyclonal Antibody, Unconjugated (bs-4188R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SGPL1) Polyclonal Antibody, Unconjugated (bs-4188R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SGPL1) polyclonal Antibody, Unconjugated (bs-4188R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-4188R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SLC29A4 Polyclonal Antibody, Unconjugated (bs-4176R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human HepG2 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human U-2OS cell lysates probed with SLC29A4 Polyclonal Antibody, Unconjugated (bs-4176R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4176R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-GnRHR Polyclonal Antibody, Unconjugated (bs-3159R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse intestine lysate L2 rat lung lysates probed with Anti Phospho-FAK (Tyr397)Polyclonal Antibody, Unconjugated (bs-3159R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 119kD. Observed band size: 55kD, 60kD, and 119kD
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse NIH\/3T3 cell lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Rat Adrenal gland lysates; Lane 6: Rat Testis lysates; Lane 7: Human 293T cell lysates; Lane 8: Human A431 cell lysates; Lane 9: Human HUVEC cell lysates; Lane 10: Human MDA-MB-231 cell lysates; Lane 11: Human A549 cell lysates probed with FAK (Tyr397) Polyclonal Antibody, Unconjugated (bs-3159R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FAK (Tyr397) Polyclonal Antibody, Unconjugated (bs-3159R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3159R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human lung carcinoma labeled with Anti-IDO Polyclonal Antibody, Unconjugated(bs-15493R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse spleen cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IDO Polyclonal Antibody(bs-15493R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IDO Polyclonal Antibody(bs-15493R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IDO) polyclonal Antibody, Unconjugated (bs-15493R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-15493R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti NGAL\/Lipocalin 2 Polyclonal Antibody, Unconjugated (bs-1373R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human brain labeled with Rabbit Anti-NGAL Polyclonal Antibody, Unconjugated(bs-1373R) 1:200 followed by conjugation to the PE secondary antibody and DAPI staining
Formalin-fixed and paraffin-embedded human brain labeled with Rabbit Anti-NGAL Polyclonal Antibody, Unconjugated(bs-1373R) 1:200 followed by conjugation to the PE secondary antibody

Catalog #1: bs-1373R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: mouse small intestine tissue labeled with Anti-TFF3 Polyclonal Antibody (bs-0535R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
HT-29 cells probed with Rabbit Anti-TFF3 Polyclonal Antibody (bs-0535R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Lane 1: mouse small intestine lysates; Lane 2: Rat small intestine lysates probed with TFF3 Polyclonal Antibody, Unconjugated (bs-0535R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TFF3) Polyclonal Antibody, Unconjugated (bs-0535R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0535R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Placenta lysates\uff1bLane 2: Mouse Lung lysates probed with AKT1\/2\/3 Polyclonal Antibody, unconjugated (bs-0115R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Tissue\/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (AKT1) polyclonal Antibody, Unconjugated (bs-0115R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0115R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Paraformaldehyde-fixed (4%) Human Endothelial Cells were blocked with normal goat serum for 20mins at 37\u2103. The cells were then labeled with Biglycan Polyclonal Antibody, Unconjugated (bs-7552R) at 1:50, overnight at 4\u00b0C. Secondary antibody incubation was performed with Goat Anti-Rabbit IgG, Rhodamine B conjugated antibody (bs-0295G-RBITC) at a 1:200 dilution for 40 minutes at 37\u00b0C. DAPI (5ug\/ml; blue) was used to stain the cell nuclei
Formalin-fixed and paraffin-embedded Human Lung Carcinoma labeled with Biglycan Polyclonal Antibody, Unconjugated (bs-7552R) at 1:200 followed by secondary antibody, DAB and counterstaining
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Biglycan Polyclonal Antibody (bs-7552R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Mouse Bone probed with Biglycan Polyclonal Antibody (bs-7552R) at 1:300, overnight at 4˚C. Followed by incubation with a conjugated secondary antibody.

Catalog #1: bs-7552R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lymphoma labeled with Rabbit Anti CRTAM Polyclonal Antibody, Unconjugated (bs-4957R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human A549 cells probed with CRTAM Polyclonal Antibody, unconjugated (bs-4957R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Catalog #1: bs-4957R

Applications: ELISA, FCM, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-CD151\/MER2\/PETA-3 Polyclonal Antibody, Unconjugated (bs-2524R) at 1:200 followed by conjugation to the secondary antibody
Mouse heart lysates probed with Rabbit Anti-CD151 Polyclonal Antibody, Unconjugated (bs-2524R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C

Catalog #1: bs-2524R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Images provided by the Independent Validation Program (badge 29798).\\n\\nLane one: HeLa cell lysates irradiated with 25 Joules\/m2\\nUV-C Lane 2: Non-irradiated HeLa cell lysates probed with Rabbit Anti-p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:100 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 37˚C.
Human MCF7 lysates probed with Rabbit Anti-p38 MAPK (Thr180+Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-p38 MAPK (Thr180 + Tyr182)) polyclonal Antibody, Unconjugated (bs-2210R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2210R

Applications: WB, ELISA, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse spleen tissue labeled with Anti CCR6\/CD196 Polyclonal Antibody, Unconjugated (bs-1542R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CCR6 Polyclonal Antibody, Unconjugated (bs-1542R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (bs-0295G-AF594) at [1:500] for 90 minutes and DAPI staining of the nuclei.
HepG2 cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CCR6 Polyclonal Antibody(bs-1542R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates probed with CCR6 Polyclonal Antibody, Unconjugated (bs-1542R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1542R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

HepG2 cells probed with Rabbit Anti-CHI3L1 Polyclonal Antibody (bs-10215R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Secondary
Human HepG2 lysates probed with CHI3L1 Polyclonal Antibody, Unconjugated (bs-10215R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Recombinant human CHI3L1 Protein (bs-41221P) lysates probed with CHI3L1 Polyclonal Antibody, Unconjugated (bs-10215R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10215R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human endometrium Anti-TrK-A\/NTRK1 Polyclonal Antibody (bs-0193R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TrkA Polyclonal Antibody, Unconjugated (bs-0193R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse Brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TrkA) Polyclonal Antibody, Unconjugated (bs-0193R) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-Cy3) for 90 minutes, and DAPI for nuclei staining.
U251 lysates probed with TrkA Polyclonal Antibody, Unconjugated (bs-0193R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0193R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti Catalase Polyclonal Antibody, Unconjugated (bs-6874R) followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates L2 rat brain lysates probed with Anti Catalase Polyclonal Antibody, Unconjugated (bs-6874R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 58kD. Observed band size: 58kD
Mouse liver lysates probed with Rabbit Anti-Catalase Polyclonal Antibody, Unconjugated (bs-6874R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Catalase Polyclonal Antibody (bs-6874R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6874R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-6985R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded Rat Colitis; Antigen retrieval performed with citrate buffer (0.01M, pH 6.0), boiling for 15min; Block endogenous peroxidase with 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37\u2103 for 20 min; Antibody incubation with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5394R) at 1:200, overnight at 4\u2103 followed by conjugated secondary antibody, DAB and counterstaining.
Lane 1: Mouse Intestinal lysate; Probed with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5394R) at 1:300 overnight at 4˚C. Followed by incubation with conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
MCF-7 cells were fixed with 70% methanol (Overnight at 4\u2103). The cells were then incubated in 1X PBS \/ 2% BSA \/ 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Primary incubation with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5349R) at 1:100 (1\u03bcg \/10^6 cells) for 30 minutes at room temp. A PE-conjugated secondary antibody (bs-0295G-PE) was used for 40 min at room temperature. The graph compares the primary antibody (green) to unstained cells (dark blue), secondary only (light blue), and isotype control (bs-0295P; orange).

Catalog #1: bs-5394R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Hela (Positive) and Molt-4 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FoxO1 (Ser256) Antibody (bs-3142R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Phospho-FoxO1 (Ser256) Polyclonal Antibody(bs-3142R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FoxO1 (Ser256) Polyclonal Antibody, Unconjugated (bs-3142R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FoxO1 (Ser256) Polyclonal Antibody, Unconjugated (bs-3142R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3142R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-TRAF3\/CD40bp Polyclonal Antibody, Unconjugated (bs-1185R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-TRAF3\/CD40bp Polyclonal Antibody, Unconjugated (bs-1185R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human Jurkat cells probed with TRAF3 Polyclonal Antibody, Unconjugated (bs-1185R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TRAF3 Polyclonal Antibody, Unconjugated (bs-1185R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1185R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

A431 (Positive) and Huvec (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CHRM4\/mAChR M4 Antibody (bs-11998R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A431 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nCHRM4\/mAChR M4 Polyclonal Antibody(bs-11998R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human U-2 OS cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human A549 cell lysates probed with CHRM4 Polyclonal Antibody, Unconjugated (bs-11998R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11998R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-11179R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human glioma labeled with Anti-IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Rat Brain lysates; Lane 2: Human Colon Carcinoma lysates; Probed with IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:200 overnight at 4˚C. Followed by secondary antibody (bs-0295G-HRP) incubation at 1:3000 for 90 min at 37˚C.
Lane 1: Human MOLT4 cell lysates; Lane 2: Human MCF-7 cell lysates probed with IGFBP2 Polyclonal Antibody, Unconjugated (bs-1108R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1108R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse tooth germ tissue labeled with Anti-Nanog Polyclonal Antibody (bs-0829R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: A549 cell lysates probed with Rabbit Anti-Nanog Polyclonal Antibody, Unconjugated (bs-0829R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
HeLa cells probed with Estrogen Receptor alpha + beta Antibody, unconjugated (bs-0829R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0829R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse brain lysates probed with MOG Polyclonal Antibody, unconjugated (bs-0426R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MOG Polyclonal Antibody, Unconjugated (bs-0426R) at 1:500 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Rat Spinal cord tissue lysates probed with MOG Polyclonal Antibody, Unconjugated (bs-0426R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0426r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GLUT1 Polyclonal Antibody (bs-0472R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image was kindly submitted by Dr. Lelisa Gemta from University of Virginia. Mouse splenocytes stained with Rabbit Anti-GLUT1 Polyclonal Antibody, PE conjugated (bs-0472R-PE)at 1:50.
Lane 1: Mouse Heart lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Muscle lysates; Lane 4: Rat Cerebrum lysates probed with GLUT1 Polyclonal Antibody, Unconjugated (bs-0472R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0472R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti EphA7\/Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Rabbit Anti-Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 107kD. Observed band size: 45kD
Lane 1: Mouse Hippocampus cell lysates probed with Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Cerebrum cell lysates probed with Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7034r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD8 Polyclonal Antibody, Unconjugated (bs-4790R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD8 Polyclonal Antibody, Unconjugated (bs-4790R) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200
U251 cells probed with CD8 Polyclonal Antibody, Unconjugated (bs-4790R) for 30 minutes followed by incubation with a conjugated secondary (FITC Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-4790R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Anti Glucagon Receptor Polyclonal Antibody, Unconjugated (bs-3945R) followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with Glucagon Receptor Polyclonal Antibody, unconjugated (bs-3945R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Glucagon Receptor Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bs-3945R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human U251 cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human MDA-MB-231 cell lysates probed with GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-16249r

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Sheep

Conjugate: Unconjugated

Catalog #1: bs-0294p-100ul

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse lung lysates probed with Anti Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 26kD. Observed band size:26kD.
Image kindly submitted by an end-user via Funakoshi. 4% Paraformaldehyde fixed HeLa cells stained with Rabbit Anti-Cytochrome C Polyclonal Antibody (bs-0013R) at 1:100 for 2 hours at room temperature
HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated (bs-0013) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).

Catalog #1: bs-0013R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-ARC Polyclonal Antibody (bs-0385R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C DAPI(5ug\/ml, blue) was used to stain the cell nuclei
A549 cells probed with Rabbit Anti-ARC\/Arg3.1 Polyclonal Antibody (bs-0385R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.\\n
Lane 1: Rat brain; Lane 2: mouse brain lysate probed with ARC Polyclonal Antibody, Unconjugated (bs-0385R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0385R

Applications: WB, ELISA, FCM

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat brain lysates probed with Rabbit Anti-RhoA(Ser188) Polyclonal Antibody, Unconjugated (bs-5330R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RhoA(Ser188) Polyclonal Antibody(bs-5330R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RhoA(Ser188) Polyclonal Antibody, Unconjugated (bs-5330R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5330R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-5072R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated