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Catalog #1: bs-8541R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bs-6505R-BF647

Applications: IF(IHC-P), IF(IHC-F)

Reactivity: Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-4834R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bs-0165R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: APC

Catalog #1: bs-0107R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-4755R-BF488

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bs-0199R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-0826R-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-0036R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-0947R-APC

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-0498R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-4690R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-4595R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bs-1074r-apc-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-0468R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: AbBy Fluor-647

Catalog #1: bs-2575R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bs-7552R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-12084R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-0163R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: PE

Catalog #1: bs-1728R-PE

Applications: WB, FCM

Reactivity: Mouse, Rat

Conjugate: PE

Catalog #1: bs-3142R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: PE

Catalog #1: bs-1852R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bs-1520R-PE

Applications: FCM

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bs-13507R-PE

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-12630R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: PE

Catalog #1: bs-7686R-PE

Applications: WB, IF

Reactivity: Human

Conjugate: PE

Catalog #1: bs-3194R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN2A\/p16-INK4a Polyclonal Antibody, Unconjugated (bs-20656R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN2A\/p16-INK4a Polyclonal Antibody, Unconjugated (bs-20656R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20656R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1468R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0648r-fitc-100ul

Applications: FCM, IF(IHC-P), Flow-Cyt

Reactivity: Human, Mouse

Conjugate: FITC

Formalin-fixed and paraffin embedded dog bladder labeled with Anti-Alpha-SMA Polyclonal Antibody, Unconjugated (bs-0189R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: dog bladder tissue labeled with Anti-Actin A\/Alpha SMA Polyclonal Antibody, Unconjugated (bs-0189R) at 1:300, followed by conjugation to the secondary antibody.
Mouse Small Intestine labeled with Anti-Actin A\/Alpha SMA Polyclonal Antibody, Unconjugated(bs-0189R) 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C
NIH\/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with alpha Actin Polyclonal Antibody(bs-0189R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0189R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Thyroid Hormone Receptor beta (bs-11440R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse liver lysates; Lane 2:Rat Liver lysates probed with THRB1 Polyclonal Antibody, Unconjugated (bs-11440R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver lysates; Lane 2: Rat Liver lysates; Lane 3: K562 cell lysates; Lane 4: HepG2 cell lysates probed with THRB1 Polyclonal Antibody, Unconjugated (bs-11440R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A431 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with THRB1 Polyclonal Antibody(bs-11440R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-11440r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0295p-cy55-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5.5

Catalog #1: bs-23539R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Flow cytometric analysis of Jurkat cells with EDG1 (1C1) Monoclonal Antibody (bsm-52902R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
SH-SY5Y cells lysates probed with EDG1 (1C1) Monoclonal Antibody (bsm-52902R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-52902r

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (Human left frontal lobe); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (NMDAR1 (13C4) ) Monoclonal Antibody, Unconjugated (bsm-52950R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (NMDAR1 (13C4) ) Monoclonal Antibody, Unconjugated (bsm-52950R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (NMDAR1 (13C4) ) Monoclonal Antibody, Unconjugated (bsm-52950R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (NMDAR1 (13C4) ) Monoclonal Antibody, Unconjugated (bsm-52950R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-52950r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

IF(ICC) staining with Phospho-JNK1\/2\/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-JNK1\/2\/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-JNK1\/2\/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-JNK1 2 3(T183+T183+T221) Monoclonal Antibody, Unconjugated(bsm-52462R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-52462r

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CENPC1 monoclonal antibody, unconjugated (bsm-54360R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed K562 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CENPC1) monoclonal Antibody, unconjugated (bsm-54360R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-54360r-TR

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: SiHa Cells; Probed with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Histone H4 (acetyl K16) (2G2) Monoclonal Antibody (bsm-54330R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54330r

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with ACLY monoclonal antibody, unconjugated (bsm-52458R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACLY Monoclonal Antibody, Unconjugated(bsm-52458R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACLY Monoclonal Antibody, Unconjugated(bsm-52458R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACLY Monoclonal Antibody, Unconjugated(bsm-52458R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-52458r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with ATF4 monoclonal antibody, unconjugated (bsm-52667R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATF4 Monoclonal Antibody, Unconjugated(bsm-52667R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATF4 Monoclonal Antibody, Unconjugated(bsm-52667R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52667r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Large intestine lysates probed with Villin-1 (5C1) Monoclonal Antibody, Unconjugated (bsm-54212R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Villin-1 (5C1)) Monoclonal Antibody, Unconjugated (bsm-54212R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.\r\n
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Villin-1 (5C1)) Monoclonal Antibody, Unconjugated (bsm-54212R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.\r\n
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Villin-1 (5C1)) Monoclonal Antibody, Unconjugated (bsm-54212R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.\r\n

Catalog #1: bsm-54212r

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

IF(ICC) staining with Growth hormone receptor (3C5) Monoclonal Antibody (bsm-54199R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Growth hormone receptor (3C5) Monoclonal Antibody (bsm-54199R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Growth hormone receptor (3C5) Monoclonal Antibody (bsm-54199R) at 1:100 in JAR cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Growth hormone receptor (3C5) Monoclonal Antibody (bsm-54199R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54199R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

IF(ICC) staining with Annexin A2 (2F4) Monoclonal Antibody (bsm-54021R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Annexin A2 (2F4) Monoclonal Antibody (bsm-54021R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Annexin A2 (2F4) Monoclonal Antibody (bsm-54021R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with Annexin A2 (2F4) Monoclonal Antibody (bsm-54021R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54021r

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52727r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bsm-52316R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
25 ug total protein per lane of various lysates (see on figure) probed with Bax monoclonal antibody, unconjugated (bsm-52316R) at 1:5000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated(bsm-52316R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

Catalog #1: bsm-52316r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of A549 cells with MMP12 (1A4) Monoclonal Antibody (bsm-52292R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).
Flow cytometric analysis of MMP12 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-52292R, 1\/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP12 Monoclonal Antibody, Unconjugated(bsm-52292R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-52292r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of NIH\/3T3 cells with DUSP6 (9C3 ) Monoclonal Antibody (bsm-52270R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with DUSP6 (9C3) Monoclonal Antibody (bsm-52270R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (DUSP6 (9C3) )Monoclonal Antibody, Unconjugated (bsm-52270R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
PC12 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (DUSP6 (9C3) )Monoclonal Antibody, Unconjugated (bsm-52270R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Catalog #1: bsm-52270r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated