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Formalin-fixed and paraffin embedded mouse embryonic bone labeled with Anti-Osteocalcin Polyclonal Antibody, Unconjugated (bs-0470R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Osteocalcin Polyclonal Antibody, Unconjugated (bs-0470R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Osteocalcin Polyclonal Antibody(bs-0470R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0470R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Raji cell lysates probed with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum cell lysates probed with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5805R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

RSC96 cells probed with Rabbit Anti-ADORA2B Polyclonal Antibody, PE Conjugated (bs-5900R-PE) at 1:30 for 40 minutes (green) compared to control cells (blue).
Mouse brain lysates probed with Rabbit Anti-ADORA2B Polyclonal Antibody (bs-5900R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADORA2B Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5900R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded Rat Colitis; Antigen retrieval performed with citrate buffer (0.01M, pH 6.0), boiling for 15min; Block endogenous peroxidase with 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37\u2103 for 20 min; Antibody incubation with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5394R) at 1:200, overnight at 4\u2103 followed by conjugated secondary antibody, DAB and counterstaining.
Lane 1: Mouse Intestinal lysate; Probed with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5394R) at 1:300 overnight at 4˚C. Followed by incubation with conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
MCF-7 cells were fixed with 70% methanol (Overnight at 4\u2103). The cells were then incubated in 1X PBS \/ 2% BSA \/ 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Primary incubation with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5349R) at 1:100 (1\u03bcg \/10^6 cells) for 30 minutes at room temp. A PE-conjugated secondary antibody (bs-0295G-PE) was used for 40 min at room temperature. The graph compares the primary antibody (green) to unstained cells (dark blue), secondary only (light blue), and isotype control (bs-0295P; orange).

Catalog #1: bs-5394R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: rat brain lysates probed with Rabbit Anti-RhoA(Ser188) Polyclonal Antibody, Unconjugated (bs-5330R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RhoA(Ser188) Polyclonal Antibody(bs-5330R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RhoA(Ser188) Polyclonal Antibody, Unconjugated (bs-5330R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5330R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD8 Polyclonal Antibody, Unconjugated (bs-4790R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD8 Polyclonal Antibody, Unconjugated (bs-4790R) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200
U251 cells probed with CD8 Polyclonal Antibody, Unconjugated (bs-4790R) for 30 minutes followed by incubation with a conjugated secondary (FITC Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-4790R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4727R-FITC

Applications: WB, FCM

Reactivity: Human

Conjugate: FITC

Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti MCR Polyclonal Antibody, Unconjugated (bs-1850R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 108kD. Observed band size: 108kD.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Mineralocorticoid receptor Polyclonal Antibody, Unconjugated (bs-1850R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nMineralocorticoid receptor Polyclonal Antibody(bs-1850R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Mineralocorticoid receptor Polyclonal Antibody(bs-1850R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1850R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11179R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse lung lysates probed with Anti Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 26kD. Observed band size:26kD.
Image kindly submitted by an end-user via Funakoshi. 4% Paraformaldehyde fixed HeLa cells stained with Rabbit Anti-Cytochrome C Polyclonal Antibody (bs-0013R) at 1:100 for 2 hours at room temperature
HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated (bs-0013) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).

Catalog #1: bs-0013R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Anti-CD10 Polyclonal Antibody (bs-0527R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
A549 cells probed with CD10 Polyclonal Antibody, Unconjugated (bs-0527R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human Hela cells probed with CD10 Polyclonal Antibody, Alexa Fluor 647 conjugated (bs-0527R-A647) (green) at 1:20 for 30 minutes compared to unstained cells (blue) and isotype control (orange).
Raji lysates probed with CD10 Polyclonal Antibody, Unconjugated (bs-0527R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-0527r

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0295p-bf647-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-647

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GLUT1 Polyclonal Antibody (bs-0472R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image was kindly submitted by Dr. Lelisa Gemta from University of Virginia. Mouse splenocytes stained with Rabbit Anti-GLUT1 Polyclonal Antibody, PE conjugated (bs-0472R-PE)at 1:50.
Lane 1: Mouse Heart lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Muscle lysates; Lane 4: Rat Cerebrum lysates probed with GLUT1 Polyclonal Antibody, Unconjugated (bs-0472R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0472R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MHC class I) Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U266 cell lysates probed with MHC class I Monoclonal Antibody, Unconjugated (bsm-52827R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52827r

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti EphA7\/Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Rabbit Anti-Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 107kD. Observed band size: 45kD
Lane 1: Mouse Hippocampus cell lysates probed with Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Cerebrum cell lysates probed with Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7034r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.01%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Calreticulin Polyclonal Antibody,FITC Conjugated(bs-5913R-FITC)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS.Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
K562 cells were incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Calreticulin Antibody(bs-5913R-FITC)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS\uff0c Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-5913R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-4194R

Applications: WB, ELISA, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-Phospho-FRAP1\/mTOR(Ser2448) Polyclonal Antibody, Unconjugated (bs-3494R) at 1:200 followed by conjugation to the secondary antibody and DAB stainin
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with mTOR (Ser2448) Polyclonal Antibody, Unconjugated (bs-3494R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with mTOR (Ser2448) Polyclonal Antibody(bs-3494R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR (Ser2448) Polyclonal Antibody, Unconjugated (bs-3494R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3494R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat small intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-LPL Polyclonal Antibody, Unconjugated (bs-1973R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining\\t\\t\\t\\t
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LPL Polyclonal Antibody, Unconjugated (bs-1973R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates probed with LPL Polyclonal Antibody, Unconjugated (bs-1973R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

Catalog #1: bs-1973R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1411R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0294p-100ul

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody (bs-0036R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse hippocampus labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated(bs-0036R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-0036R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-ARC Polyclonal Antibody (bs-0385R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C DAPI(5ug\/ml, blue) was used to stain the cell nuclei
A549 cells probed with Rabbit Anti-ARC\/Arg3.1 Polyclonal Antibody (bs-0385R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.\\n
Lane 1: Rat brain; Lane 2: mouse brain lysate probed with ARC Polyclonal Antibody, Unconjugated (bs-0385R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0385R

Applications: WB, ELISA, FCM

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52829r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RAGE Monoclonal Antibody, Unconjugated (bsm-52809R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RAGE Monoclonal Antibody, Unconjugated (bsm-52809R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RAGE Monoclonal Antibody, Unconjugated (bsm-52809R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RAGE Monoclonal Antibody, Unconjugated (bsm-52809R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52809r

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast cancer labeled with Rabbit Anti-AKT1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AKT1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-Akt1\/2\/3 (Thr305\/308\/309) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: HepG2 cell lysates probed with phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C. The upper band could be the fully glycosylated form of the target protein.

Catalog #1: bs-5182R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4947R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-4236R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-2615R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse eye labeled with Anti Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse lung lysates Lane 2: mouse muscle lysates probed with Anti Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 20kD. Observed band size: 20kD.
RSC96 cells probed with Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1453R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue with labeled Anti-CD38 Polyclonal Antibody, Unconjugated (bs-0979R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with CD38 Polyclonal Antibody, Unconjugated (bs-0979R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Rat Cerebrum lysates probed with CD38 Polyclonal Antibody, Unconjugated (bs-0979R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0979R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse brain tissue labeled with Rabbit Anti-NF-H\/Neurofilament H\/Neurofilament 200 Polyclonal Antibody (bs-0708R), Unconjugated followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat spinal cord labeled with Rabbit Anti-NF-H\/Neurofilament H\/Neurofilament 200 Polyclonal Antibody (bs-0708R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-NF-H\/Neurofilament H\/Neurofilament 200 Polyclonal Antibody (bs-0708R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (NF-H) Polyclonal Antibody, Unconjugated (bs-0708R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0708R

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-GAPDH Polyclonal Antibody, Unconjugated (bs-0459R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
This image was generously provided by Loyda M. Mel\u00e9ndez at the University of Puerto Rico Medical Sciences Campus.\\nA total of 30ug of monocyte-derived macrophage lysates were reduced and denatured in 1X Laemli Buffer with beta-mercaptoethanol. Samples were loaded into a TGX 4-20% gel, at 150V for 1 hour. Proteins were transferred to a PVDF membrane and probed for proteins of interest. The membrane was re-probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) in 5% milk at 1:250 dilution overnight at 4\u00b0C. The next day, the membrane was washed with TBS-T and incubated with goat anti-rabbit HRP conjugated secondary antibody for 1 hour at room temperature. The membrane was exposed for 18 seconds.\\n
MCF-7 cell lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.
Mouse muscle lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0459R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-Fas Polyclonal Antibody, Unconjugated (bs-0215R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Thymus tissue lysates; Lane 2: Rat Thymus tissue lysates; Lane 3: Human HepG2 cell Lysates; Lane 4: Human Raji cell Lysates; Lane 5: Human HT1080 cell Lysates; Lane 6: Human A431 cell Lysates probed with CD95\/FAS Polyclonal Antibody, Unconjugated (bs-0215R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-0215R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled withAnti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032r) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysates, L2 mouse brain lysates probed with Anti- Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. P

Catalog #1: bs-0032r

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1004R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-S100A7 Polyclonal Antibody, Unconjugated(bs-6575R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with S100A7 Antibody(bs-6575R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6575r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-6217R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-P53(wt-p53) Polyclonal Antibody, Unconjugated (bs-2090R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
MCF7 cells were fixed with 70% ice-cold methanol overnight at 4\u2103, permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with P53(wt-p53) Polyclonal Antibody at 1:50 dilution in blocking buffer and incubated for 30 min at -20\u2103, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.
Lane 1: Mouse Liver lysates; Lane 2: Rat Liver lysates probed with P53(wt-p53) Polyclonal Antibody, Unconjugated (bs-2090R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (P53(wt-p53)) polyclonal Antibody, Unconjugated (bs-2090R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2090r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hela cells probed with Giantin\/GM130 Polyclonal Antibody, Unconjugated (bs-13356R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Giantin\/GOLGB1 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human cervix cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with\\tGiantin\/GOLGB1 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Giantin Polyclonal Antibody, Unconjugated (bs-13356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Catalog #1: bs-13356R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Anti-ABCG1 Polyclonal Antibody, Unconjugated (bs-1231R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Molt4 cells(black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ABCG1 Polyclonal Antibody, Cy5.5 Conjugated (bs-1231R-Cy5.5) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 20 minutes; Antibody incubation with ABCG1 Polyclonal Antibody, Unconjugated (bs-1231R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 20 minutes; Antibody incubation with ABCG1 Polyclonal Antibody, Unconjugated (bs-1231R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

Catalog #1: bs-1231R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0522R-BF555

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

IF(ICC) staining with SCGB1A1 (7G5) Monoclonal Antibody (bsm-54221R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SCGB1A1 (7G5) Monoclonal Antibody (bsm-54221R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SCGB1A1 (7G5) Monoclonal Antibody (bsm-54221R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Flow cytometric analysis of A549 cells with SCGB1A1 (7G5) Monoclonal Antibody (bsm-54221R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Catalog #1: bsm-54221r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with Alkaline Phosphatase (1G9 ) Monoclonal Antibody (bsm-52252R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).
IF(ICC) staining with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52252r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

MCF7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HSF1 (S326) (37E4) Monoclonal Antibody(bsm-52166R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with HSF1 (S326) (37E4) Monoclonal Antibody (bsm-52166R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with HSF1 (S326) (37E4) Monoclonal Antibody (bsm-52166R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse spleen tissue incubated with HSF1 (S326) (37E4) Monoclonal Antibody (bsm-52166R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52166r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Unconjugated

HeLa cells probed with Rabbit Anti-Calreticulin Polyclonal Antibody (bs-5913R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.
Lane 1: Mouse Liver cell lysates probed with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.01%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nCalreticulin Polyclonal Antibody(bs-5913R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bs-5913R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse pancreas labeled with Rabbit Anti-ATG1\/ULK1 Polyclonal Antibody, Unconjugated(bs-3602R) 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
293T cell lysates probed with ATG1\/ULK1 Polyclonal Antibody, Unconjugated (bs-3602R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ATG1\/ULK1) polyclonal Antibody, Unconjugated (bs-3602R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-3602R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti LTB4-R2 Polyclonal Antibody, Unconjugated (bs-2655R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 40kD. Observed band size: 40kD.
Lane 1: Human U937 cell lysates; Lane 2: Human MCF-7 cell lysates probed with LTB4-R2 Polyclonal Antibody, Unconjugated (bs-2655R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2655R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated