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Formalin-fixed and paraffin embedded rat uterus labeled with Anti-STAT6 Polyclonal Antibody, Unconjugated (bs-1143R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT6 Polyclonal Antibody, Unconjugated (bs-1143R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT6 Polyclonal Antibody, Unconjugated (bs-1143R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT6 Polyclonal Antibody, Unconjugated (bs-1143R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1143R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11180R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Caspase-1 p20 Polyclonal Antibody, Unconjugated (bs-10743R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the secondary antibody and DAB staining
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Caspase-1 p20 Polyclonal Antibody(bs-10743R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-10743R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10599R

Applications: FCM

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-ADORA2B Polyclonal Antibody, Unconjugated(bs-10205R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti-ADORA2B Polyclonal Antibody, Unconjugated(bs-10205R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
RSC96 cells probed with Rabbit Anti-ADORA2B Polyclonal Antibody, PE Conjugated (bs-10791R-PE) at 1:30 for 40 minutes (green)compared to control cells (blue).

Catalog #1: bs-10205R

Applications: WB, FCM, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HeLa cells probed with Rabbit Anti-COX4 Polyclonal Antibody (bs-R) at 1:20 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG conjugated secondary FITC (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\\n
Validation submitted by One World Lab. MCF-7 cells were stained with COX4 Polyclonal Antibody, Unconjugated\uff0cbs-10257R at 1:50 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with COX4 Polyclonal Antibody, Unconjugated (bs-10257R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Mouse kidney lysates probed with COX4 Polyclonal Antibody, Unconjugated (bs-10257R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-10257R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0600R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Tubulin-Beta Polyclonal Antibody, Unconjugated (bs-0715R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Rat Cerebrum lysates probed with Beta Tubulin Polyclonal Antibody, Unconjugated (bs-0715R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0715R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Tubulin-beta Polyclonal Antibody, Unconjugated (bs-0210R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
RSC96 cells probed with Tubulin-beta Polyclonal Antibody, Unconjugated (bs-0210R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Mouse Cerebellum Lysate;Lane 2: Rat Cerebellum Lysate;Lane 3: Mouse Cerebrum Lysate;Lane 4: Rat Cerebrum Lysate;Lane 5:Mouse Ovary Lysate;Lane 6:Mouse Heart Lysate;Lane 7: Rat Heart Lysate probed with beta Tubulin Polyclonal Antibody, Unconjugated (bs-0210R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta Tubulin Polyclonal Antibody(bs-0210R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0210R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-GSK-3 Beta (CT) Polyclonal Antibody, Unconjugated (bs-0028R) 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse heart lysates probed with Anti GSK-3 Beta (CT) Polyclonal Antibody, Unconjugated (bs-0028R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 47kD. Observed band size:47kD.\\n
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-GSK-3 Beta Polyclonal Antibody (bs-0028R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
MCF-7 cells probed with GSK-3 Beta Polyclonal Antibody, unconjugated (bs-0028R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0028R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tLeptin Polyclonal Antibody, Unconjugated (bs-0108R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tLeptin Polyclonal Antibody, Unconjugated (bs-0108R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-0108R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

K562 Cell lysates probed with presenilin 1 Polyclonal Antibody, unconjugated (bs-0024R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Presenilin 1 Polyclonal Antibody, Unconjugated (bs-0024R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
THP-1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Presenilin 1 Polyclonal Antibody(bs-0024R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (presenilin 1) polyclonal Antibody, Unconjugated (bs-0024R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0024R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BACE1\/ASP2 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates probed with Anti BACE1\/ASP2 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 51kD. Observed band size:51kD.\\n
Mouse muscle lysates probed with Rabbit Anti-BACE1 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BACE1 Polyclonal Antibody, Unconjugated (bs-0164R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0164R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human uterine neck; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with P-V-Myb+C-Myb(S11) Monoclonal Antibody, Unconjugated (bsm-54494R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with P-V-Myb+C-Myb(S11) Monoclonal Antibody, Unconjugated (bsm-54494R) at 1:200 for 30 minutes at room temperature, DAB staining.
Ags cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (P-V-Myb+C-Myb(S11)) Monoclonal Antibody, Unconjugated (bsm-54494R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Fig4: Flow cytometric analysis of MCF-7 cells with P-V-Myb+C-Myb(S11) antibody(bsm-54494R) at 1\/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as antibody

Catalog #1: bsm-54494r

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Human U251 lysates probed with Tau Polyclonal Antibody, Unconjugated (bs-20445R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tau Polyclonal Antibody, Unconjugated (bs-20445R) at 1:400 overnight at 4\u00b0C, followed secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tau Polyclonal Antibody, Unconjugated (bs-20445R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.
Paraformaldehyde-fixed, paraffin-embedded Rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tau Polyclonal Antibody, Unconjugated (bs-20445R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

Catalog #1: bs-20445R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

L1 human colon carcinoma lysates L2 rat brain lysates probed with Anti- WT-1\/Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 55kD
Formalin-fixed and human kidney tissue labeled with Anti-WT-1\/Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Wilms Tumor Protein Polyclonal Antibody, Unconjugated (bs-6983R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-6983R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11961r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: SiHa Cells; Lane 2: PC-3M Cells; Lane 3: A549 Cells; Probed with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of HepG2 cells with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Catalog #1: bsm-54417r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

IF(ICC) staining with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with PSMA1 (10A2) Monoclonal Antibody (bsm-54170R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54170r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human U-2OS cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human Hela cell lysates probed with Id1 (5F1) Monoclonal Antibody, Unconjugated (bsm-54101R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ID1) monoclonal Antibody, Unconjugated (bsm-54101R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with ID1 Monoclonal Antibody(bsm-54101R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ID1 Monoclonal Antibody, Unconjugated (bsm-54101R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54101r

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-52392R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-52392r-TR

Applications: WB, IHC-P, IHC-F, IF(ICC), IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDX2 Monoclonal Antibody, Unconjugated(bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52348r

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human laryngeal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD9 (4C2) Monoclonal Antibody, Unconjugated (bsm-52264R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52264r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ERK1\/2 (3A12) Monoclonal Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Tissue\/cell:A549 cell;4% Paraformaldehyde-fixed;Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ERK1\/2) monoclonal Antibody, Unconjugated (bsm-52259R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated(bsm-52259R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated(bsm-52259R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52259r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

TT lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A431 lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
U251 lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse Stomach tissue probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-17422R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

ICC staining of c-Jun(S63) in MCF-7 cells (green) using c-Jun(S63) (6C2) Monoclonal Antibody (bsm-52141R). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
ICC staining using c-Jun(S63) (6C2) Monoclonal Antibody followed by A549 conjugated secondary antibody staining (green), and nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Human laryngeal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nc-Jun(S63) (6C2) Monoclonal Antibody, Unconjugated (bsm-52141R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nc-Jun(S63) (6C2) Monoclonal Antibody, Unconjugated (bsm-52141R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52141r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

U937 lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-20351R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bcl-2 Polyclonal Antibody(bs-20351R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Recombinant human Bcl-2 protein, C-His probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-20351R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20351R

Applications: WB, ELISA, FCM

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: PC-3 Cell lysates; Lane 2: mouse brain lysates; Lane 3: rat brain lysates; Lane 4: human brain lysates; Lane 5: human testis lysates; probed with RAB18 (3A8) Monoclonal Antibody, unconjugated (bsm-51333M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
PC-3 cells probed with using RAB18 (3A8) Monoclonal Antibody bsm-51333M at 1:25 for 60 minutes followed by A488 secondary antibody at 1:200 (green), compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51333M

Applications: WB, FCM, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7674R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Spinal Cord Lysate at 40ug; Probed with HOXB5 Polyclonal Antibody (bs-6668R, unconjugated) at 1:1000 overnight at 4\u00b0C followed by Goat Anti-Rabbit IgG at 1:20000 dilution.
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20\u2103 .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Cells stained with primary antibody HOXB5 Polyclonal Antibody (green), and isotype control (orange). Blank Control: sample.

Catalog #1: bs-6668R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-NTR3 Polyclonal Antibody, Unconjugated (bs-6329R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with Sortilin Polyclonal Antibody, Unconjugated (bs-6329R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6329R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-5854R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-5760R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRS1(Ser312) Polyclonal Antibody(bs-5396R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRS1(Ser312) Polyclonal Antibody, Unconjugated (bs-5396R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5396R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-Histone H2A.X (Tyr143) Polyclonal Antibody, Unconjugated (bs-5360R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Phospho-Histone H2A.X (Tyr143) Polyclonal Antibody, Unconjugated (bs-5360R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Histone H2A.X (Tyr143) Antibody, Unconjugated (bs-5360R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37\u00b0C for 30min; Antibody incubation with H2AX Polyclonal Antibody, Unconjugated bs-5360R at 1:400 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-5360R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Bad (Ser118) Polyclonal Antibody, Unconjugated (bs-5216R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Bad (Ser118) Polyclonal Antibody, Unconjugated (bs-5216R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela cell lysates probed with Phospho-Bad (Ser118) Polyclonal Antibody, Unconjugated (bs-5216R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: Hela KO Bad cell lysates probed with Bad (Ser118) Polyclonal Antibody, Unconjugated (bs-5216R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5216R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4291R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Raji cells probed with \\tCD21\/EBV receptor Polyclonal Antibody, Unconjugated (bs-3792R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Raji lysates probed with CD21 Polyclonal Antibody, Unconjugated (bs-3792R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3792R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-3552R

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HOXB4 Polyclonal Antibody, Unconjugated (bs-3650R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: U87MG cell lysates; Lane 2: Molt-4 cell lysates probed with HOXB4 Polyclonal Antibody, Unconjugated (bs-3650R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HOXB4 Polyclonal Antibody(bs-3650R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HOXB4 Polyclonal Antibody, Unconjugated (bs-3650R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3650R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Rat Liver lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Rat Pancreas lysates probed with GSK-3 Beta Polyclonal Antibody, Unconjugated (bs-3148R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GSK-3 Beta(Thr390) Polyclonal Antibody(bs-3148R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3148R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat breast tissue labeled with Anti-Phospho-Estrogen Receptor alpha (Ser104\/106) Polyclonal Antibody, Unconjugated (bs-3131R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-Estrogen Receptor alpha (Ser104 + Ser106) ) polyclonal Antibody, Unconjugated (bs-3131R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-3131R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2584R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2522R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2272R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2520R

Applications: WB, ELISA, FCM

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2436R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2517R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated