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Catalog #1: bsm-52309r

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Hela; Probed with CBX5 (3G1) Monoclonal Antibody (bsm-52301R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CBX5 (3G1) Monoclonal Antibody (bsm-52301R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CBX5 (3G1) Monoclonal Antibody (bsm-52301R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CBX5 (3G1) Monoclonal Antibody (bsm-52301R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52301R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse testis; Lane 2: CRC lysate probed with Histone H3(mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Histone H3 (mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Histone H3 (mono+di+methyl K79) (2B11) Monoclonal Antibody (bsm-52291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52291R

Applications: WB, FCM, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human 293T cell lysates probed with beta Tubulin Monoclonal Antibody, Unconjugated (bsm-52290R) at 1:20000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-52290r

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293; Lane 2: Jurkat; Lane 3: Mouse kidney; Lane 4: HepG2 lysate probed with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HepG2 cells with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
Hybrid fish (crucian-carp) liver tissue probed with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52282R

Applications: WB, FCM, IHC-P, IHC-F, IF(ICC)

Reactivity: Human, Mouse, Rat, Fish, Zebrafish

Conjugate: Unconjugated

293 cell lysates probed with Caspase 2 (2B1) Monoclonal Antibody (bsm-52281R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of Jurkat cells with Caspase 2 (2B1) Monoclonal Antibody (bsm-52281R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with Caspase 2 (2B1) Monoclonal Antibody (bsm-52281R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Caspase 2 (2B1) Monoclonal Antibody (bsm-52281R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52281R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with PTK2 (2F4) Monoclonal Antibody (bsm-52277R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with PTK2 (2F4) Monoclonal Antibody (bsm-52277R) at 1:100 in PANC-1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PTK2 (2F4) Monoclonal Antibody (bsm-52277R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
25 ug total protein per lane of various lysates (see on figure) probed with PTK2 monoclonal antibody, unconjugated (bsm-52277R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-52277r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HepG2; Lane 2: Hela; Lane 3: Jurkat lysate probed with \r\nRho A+B+C (2G9) Monoclonal Antibody (bsm-52275R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HepG2 cells with Rho A+B+C (2G9) Monoclonal Antibody (bsm-52275R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with Rho A+B+C (2G9) Monoclonal Antibody (bsm-52275R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with Rho A+B+C (2G9) Monoclonal Antibody (bsm-52275R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52275R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse brain; Lane 2: Human brain lysate probed with \r\nDLG4 (1H8) Monoclonal Antibody (bsm-52272R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of SH-SY-5Y cells with DLG4 (1H8) Monoclonal Antibody (bsm-52272R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with DLG4 (1H8) Monoclonal Antibody (bsm-52272R) at 1:100 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with DLG4 (1H8) Monoclonal Antibody (bsm-52272R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52272R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

IF(ICC) staining with Phospho-Histone H3 (Ser10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-Histone H3 (Ser10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-Histone H3 (Ser10) (2F6) Monoclonal Antibody (bsm-52261R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-Histone H3 (S10) Monoclonal Antibody, Unconjugated (bsm-52261R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52261r

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: Hela; Lane 4: NIH\/3T3; Probed with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of Hela cells with Filamin A antibody at 1\/50 dilution (blue) compared with an unlabelled control or cells without incubation with primary antibody (red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
IF(ICC) staining with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52260R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293T; Lane 2: NIH\/3T3; Lane 3: HepG2 lysates probed with PBR (1D4) Monoclonal Antibody (bsm-52249R) at 1:1000 dilution and incubated overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HeLa cells with PBR (1D4) Monoclonal Antibody (bsm-52249R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).
IF(ICC) staining with PBR (1D4) Monoclonal Antibody (bsm-52249R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PBR (1D4) Monoclonal Antibody (bsm-52249R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52249R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ATF2 (Thr69\/71) Polyclonal Antibody, Unconjugated (bs-3033R) at 1:200 overnight at 4\u00b0C, followed by secondary and DAB staining.
Paraformaldehyde-fixed, paraffin-embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ATF2 (Thr69\/71) Polyclonal Antibody, Unconjugated (bs-3033R) at 1:200 overnight at 4\u00b0C, followed by secondary and DAB staining.
Mouse spleen cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ATF2 (Thr69\/71) Polyclonal Antibody(bs-3033R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ATF2 (Thr69\/71) Polyclonal Antibody, Unconjugated (bs-3033R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3033R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HSP27 Polyclonal Antibody, Unconjugated (bs-20666R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HSP27 Polyclonal Antibody, Unconjugated (bs-20666R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: Hela cell lysates probed with HSP27 Polyclonal Antibody, Unconjugated (bs-20666R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20666R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti- IKK beta Polyclonal Antibody, Unconjugated (bs-4880R) at 1:200 followed by conjugation to the secondary antibody
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IKK_\/_ Polyclonal Antibody(bs-4880R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IKK beta Polyclonal Antibody, Unconjugated (bs-4880R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4880R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Cerebral cortex lysates; Lane 2: Mouse hippocampus lysates probed with MGLUR5 Polyclonal Antibody, Unconjugated (bs-18801R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse cerebrum lysates probed with MGLUR5 Polyclonal Antibody, Unconjugated (bs-18801R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with MGLUR5 Polyclonal Antibody, Unconjugated (bs-18801R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-18801R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse embryo lysates probed with Angiotensinogen Polyclonal Antibody, Unconjugated (bs-20777R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Angiotensinogen Polyclonal Antibody, Unconjugated (bs-20777R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Liver lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Kidney lysates; Lane 4: Mouse Adrenal gland lysates; Lane 5: Rat Cerebrum lysates; Lane 6: Rat Adrenal gland lysates probed with Angiotensinogen Polyclonal Antibody, Unconjugated (bs-20777R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Angiotensinogen Polyclonal Antibody(bs-20777R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-20777R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-20716R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Human MCF-7 lysates probed with Galectin 3 Polyclonal Antibody, Unconjugated (bs-20699R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Galectin 3 Polyclonal Antibody(bs-20699R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Galectin 3) polyclonal Antibody, Unconjugated (bs-20699R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-20699R

Applications: WB, ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-20577R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EIF4B (Ser406) Polyclonal Antibody, Unconjugated (bs-14551R) at 1:400 overnight at 4\u00b0C, DAB staining.
HeLa lysates probed with EIF4B (Ser406) Polyclonal Antibody, Unconjugated (bs-14551R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-14551R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7464R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Ags cell lysates probed with IKB alpha (Ser32) (13E7) Monoclonal Antibody (bsm-52169R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
MCF7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IKB alpha (Ser32) (13E7) Monoclonal Antibody(bsm-52169R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-52169r

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-11230R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: A431; Lane 2: HeLa lysate at 20 \u00b5g per lane, probed with bsm-51104M ACTA1 (337CT30.10.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ACTA1 (337CT30.10.1) Monoclonal Antibody (bsm-51104M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: K562; Lane 2: CEM lysate at 20 \u00b5g per lane, probed with bsm-51104M ACTA1 (337CT30.10.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-51104M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51095M

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: HepG2 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: NCI-H460 Cell lysates; probed with ALDH1A1 (2C2) Monoclonal Antibody, unconjugated (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: NCI-H460 Cells; 15 ug of protein lysate loaded; Probed with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:25 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin (red). Cells were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X100\/PBS.

Catalog #1: bsm-51032M

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51027M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A549 Cell lysates; Lane 2: SK-BR-3 Cell lysates; Lane 3: C2C12 Cell lysates; probed with CAPN2 (1C1) Monoclonal Antibody, unconjugated (bsm-51429M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CAPN2 (1C1) Monoclonal Antibody (bsm-51429M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Flow cytometric analysis of U-87 MG cells using bsm-51429M CAPN2 Antibody (green) compared to mouse IgG2b isotype control (blue). bsm-51429M was diluted at 1:100 dilution. Alexa Fluor\u00ae 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Catalog #1: bsm-51429M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: rat liver lysates; Lane 2: NIH\/3T3 Cell lysates; Lane 3: MCF-7 Cell lysates; Lane 4: mouse liver lysates; Lane 5: C6 Cell lysates;probed with VCP (4C4) Monoclonal Antibody, unconjugated (bsm-51420M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Fluorescent image of U251 cells stained with VCP (4C4) Antibody (bsm-51420M) at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor\u00ae 555 conjugated with Phalloidin (red).

Catalog #1: bsm-51420M

Applications: WB, FCM, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7 Cell lysates; Lane 2: A549 Cell lysates; Lane 3: A431 Cell lysates; probed with CAPN1 (6C5) Monoclonal Antibody, unconjugated (bsm-51419M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CAPN1 (6C5) Monoclonal Antibody (bsm-51419M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Hela cells probed with using CAPN1 (6C5) Monoclonal Antibody bsm-51419M at 1:25 for 60 minutes followed by A488 secondary antibody at 1:400 (green), compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51419M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: NCI-H292 Cell lysates; Lane 2: A549 Cell lysates; Lane 3: HepG2 Cell lysates; probed with ALDH2 (138CT22.3.8) Monoclonal Antibody, unconjugated (bsm-51417M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ANTIBODY Monoclonal Antibody (bsm-51417M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51417M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with YY1 (3C6) Monoclonal Antibody (bsm-51412M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U-251 cell lysates; probed with YY1 (3C6) Polyclonal Antibody, unconjugated (bsm-51412M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Monkey brain tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with YY1 (3C6) Monoclonal Antibody, Unconjugated (bsm-51412M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51412M

Applications: WB, FCM, IHC-P

Reactivity: Human, Rat, Monkey

Conjugate: Unconjugated

Lane 1: Daudi Cell lysates; Lane 2: Raji Cell lysates; probed with BCL10 (5C2) Monoclonal Antibody, unconjugated (bsm-51398M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BCL10 (5C2) Monoclonal Antibody (bsm-51398M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Hela cells probed with using BCL10 (5C2) Monoclonal Antibody (green) unconjugated bsm-51398M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG (blue).
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with BCL10 (5C2) Monoclonal Antibody, Unconjugated (bsm-51398M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51398M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
A431 cells probed with using MYO18B (3C5) Monoclonal Antibody (green) unconjugated bsm-51376M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG2b (blue).
Recombinant protein lysates probed with MYO18B (3C5) Polyclonal Antibody, unconjugated (bsm-51376M) at 1:16000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51376M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TERF2IP (4C8) Monoclonal Antibody (bsm-51368M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: HepG2 cell lysates; Lane 2: A431 cell lysates; probed with TERF2IP (4C8) Polyclonal Antibody, unconjugated (bsm-51368M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51368M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human lung lysates; probed with RYK (1C1) Monoclonal Antibody, unconjugated (bsm-51365M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
A431 cells probed with RYK (1C1) Monoclonal Antibody (green) unconjugated bsm-51365M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 (green), compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51365M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: A431 Cell lysates; Lane 3: PC-12 Cell lysates; Lane 4: COS-7 Cell lysates; Lane 5: NIH\/3T3 Cell lysates; probed with MP2K1 (8C8) Monoclonal Antibody, unconjugated (bsm-51364M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells probed with using MP2K1 (8C8) Monoclonal Antibody (green) unconjugated bsm-51364M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51364M

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: K562 Cell lysates; Lane 3: Daudi Cell lysates; probed with PLIN3 (1C4) Monoclonal Antibody, unconjugated (bsm-51361M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HUVEC cells probed with using PLIN3 (1C4) Monoclonal Antibody (green) unconjugated bsm-51361M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).
Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS cells; Antibody incubation with PLIN3 (1C4) Monoclonal Antibody, Unconjugated (bsm-51361M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). The nuclear counter stain is DAPI (blue).

Catalog #1: bsm-51361M

Applications: WB, FCM, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Jurkat Cell lysates; Lane 2: Ramos Cell lysates; Lane 3: HT-1080 Cell lysates; probed with GARS (1C6) Monoclonal Antibody, unconjugated (bsm-51350M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells; antibody incubation with GARS (1C6) Monoclonal Antibody, Unconjugated (bsm-51350M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Hela cells probed with using GARS (1C6) Monoclonal Antibody (green) unconjugated bsm-51350M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51350M

Applications: WB, FCM, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Raji Cell lysates; probed with RASSF2 (9C9) Monoclonal Antibody, unconjugated (bsm-51348M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells probed with using RASSF2 (9C9) Monoclonal Antibody bsm-51348M at 1:25 for 30 minutes followed by A488 secondary antibody at 1:200 (green) compared to an isotype control of mouse IgG2a (blue).

Catalog #1: bsm-51348M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: PC-12 cell lysate (20\u00b5g per lane) probed with bsm-51346M AKT2 (1623CT791.157.67.66) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa cells labeled with AKT2 (16C6) Monoclonal Antibody at 1:25 dilution, followed by goat anti-mouse IgG secondary antibody (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1\/100 dilution (red). Nuclear counter stain is DAPI (blue).

Catalog #1: bsm-51346M

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: MOLT-4 Cell lysates; probed with ETS1 (1C1) Monoclonal Antibody, unconjugated (bsm-51344M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cells probed with using ETS1 (1C1) Monoclonal Antibody (green) unconjugated bsm-51344M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG2b (blue).

Catalog #1: bsm-51344M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: HL-60 Cell lysates; Lane 3: U-2OS Cell lysates; probed with RAB5C (6C1) Monoclonal Antibody, unconjugated (bsm-51343M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
U-2OS cells probed with using RAB5C (6C1) Monoclonal Antibod bsm-51343M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody (green) at 1:400 compared to an isotype control of mouse IgG2b (blue).

Catalog #1: bsm-51343M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51337M

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

A431 cell lysates probed with NMI (8C9) Polyclonal Antibody, unconjugated (bsm-51329M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cells probed with using NMI (8C9) Monoclonal Antibody (green) unconjugated bsm-51329M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG1 (blue).
Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells; Antibody incubation with NMI (8C9) Monoclonal Antibody, Unconjugated (bsm-51329M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).

Catalog #1: bsm-51329M

Applications: WB, FCM, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51324m

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with SODM (9C3) Monoclonal Antibody (bsm-51319M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: human brain lysate; Lane 2: Hela cell lysate; Lane 3: MCF-7 cell lysate; Lane 4: NIH\/3T3 cell lysate; Lane 5: SH-SY5Y cell lysate; probed with SODM (9C3) Polyclonal Antibody, unconjugated (bsm-51319M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51319M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated