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Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0647R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.\\n
Mouse spleen cells probed with CD4 Polyclonal Antibody, PE Conjugated (bs-0647R-PE; purple) for 30 minutes at 1:50.

Catalog #1: bs-0647R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skeletal muscle labeled with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 and L2 rat skeletal muscle lysates probed: : Anti-F-Actin (bs-1571R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 42kDa
A549 cell lysates probed with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-1571R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NOS-2\/iNOS Polyclonal Antibody, Unconjugated (bs-2072R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-2072R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with Caspase-3 polyclonal antibody, unconjugated (bs-0081R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-0081r-100ul

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse myocardium labeled with (bs-1074R) Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Anti Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 66kD. Observed band size:66kD.\\n
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1074r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (bs-0127R) at 1:800 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (bs-0127R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 mouse liver lysates probed (bs-0127R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 21kDa.
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Anti \u2013BAX Polyclonal Antibody, Unconjugated (bs-0127R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining

Catalog #1: bs-0127R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Arctic Ground Squirrel splenocytes stained with Anti-PPAR alpha Polyclonal Antibody, PE-CY5 Conjugated (bs-3614R-PE-Cy5) at 1:50.
Rat splenocytes stained with Anti-PPAR alpha Polyclonal Antibody, PE-CY5 Conjugated (bs-3614R-PE-Cy5) at 1:50.
Lane 1: Mouse heart lysates Lane 2: Mouse liver lysates probed with Rabbit Anti-PPAR alpha Polyclonal Antibody, Unconjugated (bs-3614R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse heart lysates probed with Rabbit Anti-PPAR alpha Polyclonal Antibody, Unconjugated (bs-3614R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Catalog #1: bs-3614R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (bs-0465R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated (bs-0465R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).

Catalog #1: bs-0465R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse heart tissue labeled Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated(bs-0295G-AF488)used at 1:200 dilution for 40 minutes at 37\u00b0C
Mouse brain lysates probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Mouse lung lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.

Catalog #1: bs-0646r-TR

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse ovary lysates L2 mouse brain lysates probed with Anti StAR\/StARD1 Polyclonal Antibody, Unconjugated (bs-3570R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 32kD. Observed band size:32kD.\\n
Lane 1: Mouse testis lysates probed with StAR Polyclonal Antibody (bs-3570R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-3570R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Caspase-9 Polyclonal Antibody, Unconjugated(bs-0049R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Caspase-9 Polyclonal Antibody, Unconjugated(bs-0049R) 1:200 followed by conjugation to the secondary antibody and DAB staining
RSC96 probed with Caspase 9 Polyclonal Antibody, Unconjugated (bs-0049R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
HepG2 cells were stained with Caspase 9 Polyclonal Antibody, Unconjugated (bs-0049R) at 1:200 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L), FITC conjugated secondary antibody. DAPI staining of the nucleus in blue.

Catalog #1: bs-0049R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti NOX2\/gp91phox Polyclonal Antibody, Unconjugated (bs-3889R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-NOX2\/gp91phox Polyclonal Antibody, Unconjugated (bs-3889R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse spleen lysates probed with NOX2\/gp91phox Polyclonal Antibody, unconjugated (bs-3889R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Lane 1: Rat Bronchus tissue lysates; Lane 2: Rat Esophagus tissue lysates; Lane 3: Human HL60 cell lysates probed with NOX2 Polyclonal Antibody, Unconjugated (bs-3889R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3889R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse lung lysates probed with Rabbit Anti-NFkB p65 (Ser536) Polyclonal Antibody, Unconjugated (bs-0982R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated (bs-0982R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated (bs-0982R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C
HL-60 cells probed with NFKB p65(Ser536) Polyclonal Antibody, unconjugated (bs-0982R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0982R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-3597R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti PARP (N-Terminus) Polyclonal Antibody, Unconjugated(bs-2138R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI
Lane 1: Rat Brain; Probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 in 4˚C.
Human HeLa lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Human Raji lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-2138R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat thymus tissue labeled Anti-PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
293T lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A549 lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human MOLT-4 cell lysates; Lane 4: Mouse NIH\/3T3 cell lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Rat Large intestine lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0754R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-phospho-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human stomach cancer tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Kindey lysates probed with STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1658R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated(bs-1992R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (bs-1992R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE)and DAPI staining
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR\/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR\/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1992R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat transplant lymphoma labeled with Anti-Caspase-1 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF-7 Cells lysates probed with Caspase-1 P10 Polyclonal Antibody, unconjugated (bs-0169R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Caspase-1 P10 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: U-87MG Cells; 30ug loaded into the lane; Probed with Caspase-1 P10 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-0169R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Desmin Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse heart tissue labeled with Anti-Desmin Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat intestine lysates probed with Anti Desmin\/DES Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 52kD. Observed band size:52kD.\\n

Catalog #1: bs-1026R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Images provided by the Independent Validation Program (badge 29798).\\n\\nLane one: HeLa cell lysates irradiated with 25 Joules\/m2\\nUV-C Lane 2: Non-irradiated HeLa cell lysates probed with Rabbit Anti-p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:100 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 37˚C.
Human MCF7 lysates probed with Rabbit Anti-p38 MAPK (Thr180+Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-p38 MAPK (Thr180 + Tyr182)) polyclonal Antibody, Unconjugated (bs-2210R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody, Unconjugated (bs-2210R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2210R

Applications: WB, ELISA, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Chicken, Goat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse lung lysates probed with Anti Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 26kD. Observed band size:26kD.
Image kindly submitted by an end-user via Funakoshi. 4% Paraformaldehyde fixed HeLa cells stained with Rabbit Anti-Cytochrome C Polyclonal Antibody (bs-0013R) at 1:100 for 2 hours at room temperature
HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated (bs-0013) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).

Catalog #1: bs-0013R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human ovary carcinoma labeled Anti-CDKN1B\/P27kip1 Polyclonal Antibody, Unconjugated (bs-0742R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
RSC96 probed with CDKN1B Polyclonal Antibody, Unconjugated (bs-0742R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
MCF-7 cells were stained with CDKN1B Polyclonal Antibody, Unconjugated(bs-0742R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Mouse lung lysates probed with CDKN1B Polyclonal Antibody, unconjugated (bs-0742R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-0742R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GAD65) polyclonal Antibody, Unconjugated (bs-0400R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Hippocampus tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates; Lane 6: Rat Hippocampus tissue lysates; Lane 7: Human SH-SY5Y cell lysates; Lane 8: Human U87MG cell lysates; Lane 9: Human HeLa cell lysates; Lane 10: Human Jurkat cell lysates probed with GAD65 Polyclonal Antibody, Unconjugated (bs-0400R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0400R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled withAnti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032r) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysates, L2 mouse brain lysates probed with Anti- Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. P

Catalog #1: bs-0032r

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0646r-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Formalin-fixed and paraffin-embedded human lung carcinoma labeled with EGFR Polyclonal Antibody, Unconjugated (bs-0165R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGFR Polyclonal Antibody, Unconjugated (bs-0165R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Tissue\/cell: Human Rectal Carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37\u2103 for 20 min; Incubation: EGFR Polyclonal Antibody, Unconjugated (bs-0165R) at 1:200, overnight at 4\u00b0C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI(5ug\/ml, blue) was used to stain the cell nuclei.
Human HUVEC Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 0.5% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min. Cells were stained with EGFR Polyclonal Antibody, Unconjugated (bs-0165R) at 1:100 for 30 min on ice. The secondary antibody, Goat anti-rabbit IgG-PE, was used for 30 min on ice. Primary Antibody staining (green) is compared to unstained cells (dark blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-0165R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody, Unconjugated (bs-1186R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Image kindly submitted by Brittany Baldwin-Murphy from Cornell University.\\n\\nChick embryo liver hepatocytes probed with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody at 1:100 or 1:500.\\n
Mouse liver cells probed with Rabbit Anti- Scavenger receptor class B member 1 Polyclonal Antibody (bs-1186R) at 1:100 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG PE conjugated secondary (bs-0295G-PE) at 1:100 (green) for 40 minutes compared to control cells (blue).
HepG2 lysates probed with Scavenger receptor class B member 1 Polyclonal Antibody, unconjugated (bs-1186R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.

Catalog #1: bs-1186R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-11514R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Cyclin B1 Polyclonal Antibody, Unconjugated (bs-0572R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HeLa cells probed with Rabbit Anti-Cyclin B1 Polyclonal Antibody (bs-0572R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.\\n
HeLa lysates probed with Anti \u2013(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.
U937 lysates probed with Anti \u2013(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0572R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0432R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded chicken ileum labeled with Anti-CD44V6 Polyclonal Antibody, Unconjugated (bs-2507R) at 1:300 followed by conjugation to the secondary antibody
Rat lung lysate probed with Anti CD44 Polyclonal Antibody, Unconjugated (bs-2507R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 80kD. Observed band size: 80kD
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD44 Polyclonal Antibody, Unconjugated (bs-2507R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD44 Polyclonal Antibody(bs-2507R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2507R

Applications: ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

\"Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-HSP-70 Polyclonal Antibody (bs-0126R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n\"
Generously provided by Merlin Levine from National Institutes of Health as part of the Bioss Discovery Program. Mouse brain labeled with Anti-HSP70 Polyclonal Antibody, Unconjugated (bs-0126R) at 1:100 overnight. The secondary antibody was AlexaFluor 555 goat anti mouse IgG1 used at 1:250 for one hour.
Mouse bladder lysates probed with HSP70 Polyclonal Antibody, Unconjugated (bs-0126R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (bs-0126R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0126R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat heart tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-SLC11A2 Polyclonal Antibody, Unconjugated (bs-3577R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Mouse adrenal gland lysates probed with DMT1 Polyclonal Antibody, Unconjugated (bs-3577R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SLC11A2 Polyclonal Antibody(bs-3577R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3577R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-NCAM1 Polyclonal Antibody, Unconjugated (bs-0736R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Human HeLa lysates probed with Rabbit Anti-CD56 Polyclonal Antibody, Unconjugated (bs-0736R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0736R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-C-Jun Polyclonal Antibody (bs-0670R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-c-Jun Polyclonal Antibody (bs-0670R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-c-Jun Polyclonal Antibody (bs-0670R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with c-Jun Polyclonal Antibody, Unconjugated (bs-0670R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-0670R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse liver lysates probed with Anti-APOA1 Polyclonal Antibody, Unconjugated (bs-0849R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-APOA1 Polyclonal Antibody, Unconjugated (bs-0849R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining.

Catalog #1: bs-0849R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-HSP70\/HSPA1A Polyclonal Antibody, Unconjugated (bs-0244R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with HSP70 1A\/1B Polyclonal Antibody, Unconjugated (bs-0244R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HSP70 Antibody(bs-0244R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Testis lysates; Lane 2: Mouse Epididymis lysates; Lane 3: Mouse Seminal vesicle lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse NIH\/3T3 cell lysates; Lane 6: Rat Testis lysates; Lane 7: Rat Spleen lysates; Lane 8: Human Hela cell lysates; Lane 9: Human Jurkat cell lysates; Lane 10: Human HepG2 cell lysates; Lane 11: Human A549 cell lysates; Lane 12: Human MCF-7 cell lysates probed with HSP70 1A\/1B Polyclonal Antibody, Unconjugated (bs-0244R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0244R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti JNK1\/2\/3 Polyclonal Antibody, Unconjugated (bs-2592R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with JNK1+2+3 Polyclonal Antibody, Unconjugated (bs-2592R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum lysates;Lane 2: Rat Cerebrum lysates;Lane 3: Mouse Cerebellum lysates; Lane 4: Mouse heart lysates probed with JNK1+2+3 Polyclonal Antibody, Unconjugated (bs-2592R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse spleen lysates probed with JNK1+2+3 Polyclonal Antibody, Unconjugated (bs-2592R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2592R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Rabbit Anti-PCNA [Proliferation Marker] Polyclonal Antibody (bs-2007R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded human gastric cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (bs-2007R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA [Proliferation Marker] Polyclonal Antibody, Unconjugated (bs-2007R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA [Proliferation Marker] Polyclonal Antibody, Unconjugated (bs-2007R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2007R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

RSC96 probed with Mfn1 Polyclonal Antibody, Unconjugated (bs-0557R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Mitofusin 1 Polyclonal Antibody, Unconjugated (bs-0557R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Mitofusin 1 Polyclonal Antibody, Unconjugated (bs-0557R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-0557R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Rat

Conjugate: Unconjugated

Lane 1: mouse heart lysates Lane 2: mouse S\/P20 cell lysates probed with Anti phospho-ERK1(Thr202\/Tyr204)+ERK2(Thr183\/Tyr185) Polyclonal Antibody, Unconjugated (bs-1646R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size:42kD
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-ERK1(Thr202\/Tyr204)+ERK2(Thr183\/Tyr185) Polyclonal Antibody, Unconjugated (bs-1646R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1(T202\/Y204)+ERK2(T183\/Y185) Polyclonal Antibody, Unconjugated (bs-1646R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1(T202\/Y204)+ERK2(T183\/Y185) Polyclonal Antibody, Unconjugated (bs-1646R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1646R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and rat colon tissue labeled with Anti-CK8 Polyclonal Antibody, Unconjugated (bs-1106R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and mouse stomach tissue labeled with Anti-CK8 Polyclonal Antibody, Unconjugated (bs-1106R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1106R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1074r-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0646r-pe-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-0169R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0049R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-JNK1\/3 Polyclonal Antibody (bs-0501R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-JNK1 + 3 Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tJNK1 + 3 Polyclonal Antibody, Unconjugated (bs-0501R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with JNK1+3 Polyclonal Antibody, Unconjugated (bs-0501R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0501R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated