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Formalin-fixed and paraffin-embedded rat colon labeled with Rabbit Anti-CD163\/M130 Polyclonal Antibody, Unconjugated( bs-2527R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CD163\/M130 Polyclonal Antibody, Unconjugated (bs-2527R) followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 mouse uterus lysates probed (bs-2527R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 130kDa.
Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163\/M130 Polyclonal Antibody, unconjugated (bs-2527R) at 1:100 for 40 minutes at 37\u00b0C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37\u00b0C. DAPI nuclear stain employed. Image shows membrane staining of testicular macrophages in the interstitial compartment of the testis, while cells in the seminiferous tubules are negative. \\n

Catalog #1: bs-2527R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. MCF-7 cells probed with Rabbit Anti-Collagen I\uffa0Polyclonal antibody (bs-10423R)at 1:1000.
This image was kindly submitted by Kandy Velazquez of USC School of Medicine.\r\nMouse adipose tissue lysates probed with Collagen I Polyclonal Antibody, unconjugated (bs-10423R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at room temperature.
Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Collagen I Antibody, Alexa Fluor 647 Conjugated (bs-10423R-AF647) at 1:400, for 1.5 hours at 37\u00b0C.
Lane 1: Mouse Ovary; Lane 2: NIH\/3T3; Lane 3: Rat Liver; Lane 4: 293T; Lane 5: A431; Lane 6: RAW264.7; Probed with Collagen I Polyclonal Antibody (bs-10423R) at 1:300 dilution overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n

Catalog #1: bs-10423r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Rat lung lysate 30ug, probed (bs-0061R) at 1:20000 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

MCF-7 cells probed with Rabbit Anti-Integrin Alpha V + Beta 3 (CD51+CD61) Polyclonal Antibody (bs-1310R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Human U937 probed with \\tIntegrin Alpha V + Beta 3 Antibody, Unconjugated (bs-1310R ) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
HUVEC cell lysates probed with Integrin Alpha V + Beta 3 (CD51+CD61) Polyclonal Antibody, Unconjugated (bs-1310R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Invasive urothelial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin Alpha V + Beta 3 (CD51+CD61) Polyclonal Antibody, Unconjugated (bs-1310R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-1310r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0647R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.\\n
Mouse spleen cells probed with CD4 Polyclonal Antibody, PE Conjugated (bs-0647R-PE; purple) for 30 minutes at 1:50.

Catalog #1: bs-0647R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-IFN-gamma Polyclonal Antibody, Unconjugated (bs-0480R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Diseased mouse lung and spleen lysates probed with Anti-IFN gamma Polyclonal Antibody (bs-0480R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Lane 1: Mouse Raw264.7-PMA Lysates; Lane 2: Mouse Lymph Lysates; Lane 3: Mouse Spleen Lysates; Lane 4: Mouse Thymus Lysates; Lane 5: Rat Lymph Lysates; Lane 6: Rat Thymus Lysates; Lane 7: Human Jurkat-TPA cell Lysates; Lane 8: Human Jurkat cell Lysates; Lane 9: Human HepG2 cell Lysates; Lane 10: Recombinant human IFN gamma Protein (100ng) (bs-0388P). Probed with IFN gamma polyclonal Antibody, unconjugated (bs-0480R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
ctll-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IFN gamma) polyclonal Antibody, Unconjugated (bs-0480R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0480r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 mouse lung lysates L2 protein provided by the end user lysates probed with Anti IL-6 Polyclonal Antibody, Unconjugated (bs-0782R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 23kD. Observed band size:23kD.

Catalog #1: bs-0782R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human artery labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (bs-1756R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human glioma labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (bs-1756R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 mouse intestine lysates L2 rat lung lysates probed with Anti Alpha Elastin Polyclonal Antibody, Unconjugated (bs-1756R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 66kD. Observed band size: 66kD
Formalin-fixed and paraffin embedded rat intervertebral disk labeled with Anti-ELASTIN, Unconjugated(bs-1756R) followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1756r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed: Endothelial cells labeled with Anti-CD31\/PECAM-1 Polyclonal Antibody, Unconjugated (bs-0468R) at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37\u00b0
Rat spleen cells probed with Rabbit Anti-CD31 Polyclonal Antibody (bs-0468R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Recombinant human CD31 protein probed with CD31 Polyclonal Antibody, unconjugated (bs-0468R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Human Jurkat cells probed with CD31 Polyclonal Antibody, Unconjugated (bs-0468R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Catalog #1: bs-0468R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Dog

Conjugate: Unconjugated

L1 rat brain lysates L2 rat heart lysates probed with Anti CD86\/B7-2 Polyclonal Antibody, Unconjugated (bs-1035R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 34kD. Observed band size:34kD.
Mouse Brain cells probed with CD86 Polyclonal Antibody, PE Conjugated (bs-1035R-PE; green) at 1:50 compared to isotype control (bs-0295P-PE; orange).
Raji cell lysates probed with CD86 Polyclonal Antibody, Unconjugated (bs-1035R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Human U937 Cells were fixed with 2% PFA (10min at room temperature). The cells were then incubated in 1X PBS containing 0.5% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Staining was performed with CD86 Polyclonal Antibody, Unconjugated (bs-1035R) at 1:100 for 30 min on ice. A Goat Anti-rabbit IgG-PE secondary antibody was used for a 30 min incubation on ice. Primary antibody staining (green) is compared to unstained cells (dark blue), secondary antibody only (light blue) and isotype control (bs-0295P; orange).

Catalog #1: bs-1035R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat skeletal muscle labeled with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 and L2 rat skeletal muscle lysates probed: : Anti-F-Actin (bs-1571R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 42kDa
A549 cell lysates probed with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-1571R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat myocardium labeled with Anti-MuRF1\/Trim63 Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and rat colitis tissue labeled with Anti-CD68 Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5),
Paraformaldehyde-fixed, paraffin-embedded Mouse Kidney issue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with CD68 Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0649R

Applications: ELISA, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti- ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) followed by conjugation to the secondary antibody and DAB staining
Image kindly submitted by Kamlesh Gupta as part of the free sample program. Caco-2 cell lysate and mouse kidney lysate probed with ZO-1 primary antibody (bs-1329R) at 1:500 overnight at 4˚C. Followed by incubation with anti-rabbit HRP-conjugated secondary antibody at 1:5000 dilution for 1h at 25˚C. Predicted and observed band size: ~200kDa.
Image provided by the Independent Validation Program, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) at 1:250 followed by conjugation to the secondary antibody.
293T (Positive) and HL-60 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody( bs-1329R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1329R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: mouse intestine lysates Lane 2: mouse liver lysates probed with Anti CD161c\/NK1.1 Polyclonal Antibody, Unconjugated (bs-4682R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 25kD. Observed band size: 25kD.
Mouse plasma probed with Rabbit Anti-CD161c Polyclonal Antibody, Unconjugated (bs-4682R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD161c\/NK1.1 Polyclonal Antibody, Unconjugated (bs-4682R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Lane 1: Human U937 cell Lysates; Lane 2: Human Jurkat cell Lysates; Lane 3: Human MOLT4 cell Lysates; Lane 4: Human MCF-7 cell Lysates. Probed with CD161c polyclonal Antibody, unconjugated (bs-4682R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4682R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Human U937 cells probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:20 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human Jurkat cells probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
U937 lysates probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates probed with CD31 Polyclonal Antibody, Unconjugated (bs-0195R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0195R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma labeled Anti-HIF-1-Alpha Polyclonal Antibody (bs-0737R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
L1 mouse heart lysates, L2 mouse large intestine lysates probed with Anti HIF-1 Alpha Polyclonal Antibody, Unconjugated (bs-0737R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 92kD
KLN 205 cells labeled with Rabbit HIF-1 Alpha Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-0737R) followed by a FITC Conjugated Secondary Antibody.
Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. Rat brain and spleen lysates probed with Rabbit Anti-HIF-1 Alpha Polyclonal Antibody (bs-0737R) at 1:1000.

Catalog #1: bs-0737R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NOS-2\/iNOS Polyclonal Antibody, Unconjugated (bs-2072R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-2072R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with Caspase-3 polyclonal antibody, unconjugated (bs-0081R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-0081r-100ul

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti OPGL\/RANKL\/ODF Polyclonal Antibody, Unconjugated (bs-0747R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti OPGL\/RANKL\/ODF Polyclonal Antibody, Unconjugated (bs-0747R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human raji cells probed with RANKL Polyclonal Antibody, Unconjugated (bs-0747R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
mouse splenocytes cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RANKL Polyclonal Antibody(bs-0747R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0747R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-PERK(Thr980) Polyclonal Antibody, Unconjugated (bs-3330R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human lung carcinoma labeled with Anti-Phospho-PERK(Thr980) Polyclonal Antibody, Unconjugated(bs-3330R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PERK (Thr980) Polyclonal Antibody, Unconjugated (bs-3330R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PERK(Thr980) Polyclonal Antibody, Unconjugated (bs-3330R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3330R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat ovary tissue labeled with Rabbit Anti-TLR2\/CD282 Polyclonal Antibody (bs-1019R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat ovary tissue labeled with Rabbit Anti-TLR2\/CD282 Polyclonal Antibody (bs-1019R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Human A549 cells probed with Rabbit Anti-TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).
Mouse spleen cells probed with \\tTLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1019R

Applications: WB, FCM, IHC-P, IHC-F, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Mouse Kidney lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1495R

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

This image was generously provided by Adib Zendedel, PhD from RWTH Aachen University. Formalin-fixed and paraffin embedded rat spinal cord tissue labeled with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:300 followed by conjugation to a secondary antibody
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Cryopyrin Polyclonal Antibody, ALEXA FLUOR\u00ae 488 Conjugated (bs-10021R-A488) 1:400, 90 minutes at 37\u00b0C; DAPI (5ug\/ml, blue) was used to stain the cell nuclei.
Lane 1: Raji lysates; Lane 2: MDA-MB-231 probed with Cryopyrin Polyclonal Antibody (bs-10021R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
SiHa lysates probed with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated (bs-10021R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-10021r-TR

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lung labeled with (bs-0812R) Rabbit Anti-IL-1 Beta\/IL-1B Polyclonal Antibody, Unconjugated followed by conjugation to the secondary antibody and DAB staining.
Image was kindly submitted by Dr.Rachel Reith, NIH. Paraffin-embedded mouse brain stained with Rabbit Anti-IL-1 Beta Polyclonal Antibody,1:100 overnight at 4C.\\n
Paraformaldehyde-fixed, paraffin-embedded Human Cervical Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C. Followed by incubation with the secondary antibody, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C. Followed by incubation with a secondary antibody, DAB and counterstaining.

Catalog #1: bs-0812r-TR

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat renal tissue labeled with Anti-TGF-beta-1 Polyclonal Antibody, Unconjugated(bs-0086R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:500 overnight at 4\u00b0C, DAB staining.
Lane 1: Isolated Transforming growth factor beta-1; Probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Embryo; Lane 2: Rat Cerebrum; Probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:1000 dilution, overnight at 4˚C. Followed by secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0086R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue Anti-GFAP Polyclonal Antibody, Unconjugated (bs-0199R) at 1:400, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: human placenta tissue labeled with Anti-GFAP Polyclonal Antibody, Unconjugated (bs-0199R) at 1:800, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-GFAP Polyclonal Antibody (bs-0199R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-GFAP Polyclonal Antibody (bs-0199R) at 1:200 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n

Catalog #1: bs-0199R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Image provided by One World Lab validation program. U138 cells were stained with bs-0756R Rabbit Anti-Vimentin Polyclonal Antibody at 1:100 dilution.

Catalog #1: bs-0756R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated(bs-0279R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Cells labeled with Anti-VEGF Polyclonal Antibody (bs-0279R).
Human colon carcinoma lysates probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 23,46kDa
HeLa cells probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated.\\n

Catalog #1: bs-0279r

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rabbit meniscus tissue labeled Anti-Collagen II Polyclonal Antibody, Unconjugated (bs-0709R) at 1:200, followed by conjugation to the secondary Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. and DAPI
Lane 1: Human 293T lysates; Lane 2: Human A549 lysates; Lane 3: Human MCF-7 lysates; Lane 4: Human HepG2 lysates; Lane 5: Mouse Placenta lysates; Lane 6: Human Mg63 lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-0709R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0709R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat lung labeled with Anti-CXCL10 Polyclonal Antibody, Unconjugated (bs-1502R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Raw264.7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL10 Polyclonal Antibody(bs-1502R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1502R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-BDNF Polyclonal Antibody, Unconjugated (bs-4989R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
U251 cells; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37C for 20 min; Antibody incubation with BDNF Polyclonal Antibody, Unconjugated (bs-4989R) 1:200, 90 minutes at 37C; followed by a conjugated secondary antibody (bs-0295G-FITC) at 37C for 90 minutes, DAPI (5ug\/ml) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with BDNF Polyclonal Antibody (bs-4989R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
HepG2 cells were fixed with 4% PFA for 10min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BDNF Polyclonal Antibody(bs-4989R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), no antibody control control (blue), secondary antibody only (light blue) and isotype control (orange).

Catalog #1: bs-4989R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human liver carcinoma tissue labeled with Anti-P-CK\/Cytokeratin AE1+AE3 Polyclonal Antibody, Unconjugated (bs-1712R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:400 overnight at 4\u00b0C, DAB staining.
Mouse Bladder lysates probed with Pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1712R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Human Siha cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HepG2 cell lysates probed with CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD14) Polyclonal Antibody, Unconjugated (bs-1192R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1192R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse myocardium labeled with (bs-1074R) Rabbit Anti-Nrf2 Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Anti Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 66kD. Observed band size:66kD.\\n
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nrf2 Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1074r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: U87MG human cell lysates probed with GPR55 Polyclonal Antibody, Unconjugated (bs-7686R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U251 cell lysates; Lane 2: Human U-2OS cell lysates probed with GPR55 Polyclonal Antibody, Unconjugated (bs-7686R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U87MG cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human U2os cell lysates probed with GPR55 Polyclonal Antibody, Unconjugated (bs-7686R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GPR55 Polyclonal Antibody, Unconjugated (bs-7686R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-7686R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Antibody\uff08bs-4727R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Polyclonal Antibody(bs-4727R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-4727r

Applications: WB, ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-3669R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-3669R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti BRN3A Polyclonal Antibody, Unconjugated (bs-3669R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 43kD. Observed band size: 43kD.
Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody(bs-3669R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3669R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-Integrin Alpha V + Beta 6 Polyclonal Antibody, Unconjugated (bs-5791R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cells probed with Integrin Alpha V + Beta 6 Antibody, Unconjugated (bs-5791R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse kidney lysates probed with Integrin Alpha V + Beta 6 Polyclonal Antibody, Unconjugated (bs-5791R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Siha cell lysates probed with Integrin Alpha V + Beta 6 Polyclonal Antibody, Unconjugated (bs-5791R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5791R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0107R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody and DAPIstaining
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody

Catalog #1: bs-2013R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-GPR43 Polyclonal Antibody, Unconjugated (bs-13536R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
THP1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GPR43 Polyclonal Antibody(bs-13536R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GPR43) polyclonal Antibody, Unconjugated (bs-13536R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-13536r

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2639R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat embryonic rhabdomyoma labeled with Anti-MyoD1\/Myf3 Polyclonal Antibody, Unconjugated (bs-2442R) at 1:200 followed by conjugation to the secondary antibody
Lane 1: mouse muscle lysates; Lane 2: mouse heart lysates probed with MyoD1 Polyclonal Antibody, Unconjugated (bs-2442R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Rat muscle lysates; Lane 2: Mouse muscle lysates; Lane probed with MyoD1 Polyclonal Antibody, unconjugated (bs-2442R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Skeletal muscle Lysates; Lane 2: Mouse Tongue Lysates; Lane 3: Rat Skeletal muscle Lysates; Lane 4: Rat Tongue Lysates. Probed with MyoD1\/Myf3 polyclonal Antibody, unconjugated (bs-2442R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2442R

Applications: WB, ELISA, FCM, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human cervical carcinoma labeled with Rabbit Anti-Ki-67 Polyclonal Antibody, Unconjugated(bs-2130R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human oral squamous cell carcinoma labeled with Rabbit Anti-Ki-67 Polyclonal Antibody, Unconjugated(bs-2130R) at 1:200 followed by conjugation to the secondary antibody and DAPI staining
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA embryonic mouse bone labeled with Anti-Ki-67 (Proliferation Marker) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Ki-67 (Proliferation Marker) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining.

Catalog #1: bs-2130R

Applications: IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10233r

Applications: WB, FCM, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Thy-1\/CD90\/ Thy1.1 Polyclonal Antibody, Unconjugated (bs-0778R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse brain lysates probed with CD90 Polyclonal Antibody, Unconjugated (bs-0778R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0778R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (bs-0127R) at 1:800 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (bs-0127R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 mouse liver lysates probed (bs-0127R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 21kDa.
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Anti \u2013BAX Polyclonal Antibody, Unconjugated (bs-0127R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining

Catalog #1: bs-0127R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse intestine lysates Lane 2: mouse lung lysates probed with Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 22kD. Observed band size: 22kD.
Lane 1: U937 cell lysates probed with CD69 Polyclonal Antibody, Unconjugated (bs-2499R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2499R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated