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Catalog #1: bs-7723R

Applications: WB, ELISA, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse brain lysates probed with Anti Cyclin M2 Polyclonal Antibody, Unconjugated (bs-6912R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cyclin M2 Antibody (bs-6912R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6912R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Bad (Ser118) Polyclonal Antibody, Unconjugated (bs-5216R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Bad (Ser118) Polyclonal Antibody, Unconjugated (bs-5216R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela cell lysates probed with Phospho-Bad (Ser118) Polyclonal Antibody, Unconjugated (bs-5216R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: Hela KO Bad cell lysates probed with Bad (Ser118) Polyclonal Antibody, Unconjugated (bs-5216R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5216R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4943R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-4187R-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Raji cells probed with \\tCD21\/EBV receptor Polyclonal Antibody, Unconjugated (bs-3792R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Raji lysates probed with CD21 Polyclonal Antibody, Unconjugated (bs-3792R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3792R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti MDH1 Polyclonal Antibody, Unconjugated (bs-3996R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: MOLT-4 cell lysates; Lane 2: Jurkat cell lysates probed with MDH1 Polyclonal Antibody, Unconjugated (bs-3996R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat cerebral cortex lysates probed with MDH1 Polyclonal Antibody, Unconjugated (bs-3996R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Stomach tissue lysates; Lane 5: Rat Heart tissue lysates; Lane 6: Rat Cerebrum tissue lysates; Lane 7: Rat Stomach tissue lysates; Lane 8: Human HL-60 cell lysates; Lane 9: Human MOLT4 cell lysates probed with MDH1 Polyclonal Antibody, Unconjugated (bs-3996R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3996R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3889R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PKC delta (Thr505\/507) Polyclonal Antibody, Unconjugated (bs-3727R) at 1:200 overnight at 4\u00b0C, DAB staining.
Raji cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PKC delta (Thr505\/507) Polyclonal Antibody(bs3727R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PKC delta (Thr505\/507) Polyclonal Antibody, Unconjugated (bs-3727R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PKC delta (Thr505\/507) Polyclonal Antibody, Unconjugated (bs-3727R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3727R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3552R

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti BETA(TYR1345) Polyclonal Antibody,Unconjugated (bs-3492R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: 293T cell lysates probed with \r\nInsulin Receptor Beta (Tyr1355) Polyclonal Antibody, Unconjugated (bs-3492R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver lysates; Lane 2: Mouse Muscle lysates; Lane 3: Rat Muscle lysates probed with Insulin Receptor Beta Polyclonal Antibody, Unconjugated (bs-3492R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3492R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HOXB4 Polyclonal Antibody, Unconjugated (bs-3650R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: U87MG cell lysates; Lane 2: Molt-4 cell lysates probed with HOXB4 Polyclonal Antibody, Unconjugated (bs-3650R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HOXB4 Polyclonal Antibody(bs-3650R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HOXB4 Polyclonal Antibody, Unconjugated (bs-3650R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3650R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human HeLa cells probed with MYPT1 (Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
293T lysates probed with MYPT1 (Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Rat BRL-3A lysates probed with p-MYPT1(Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Testis tissue lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human MCF-7 cell lysates probed with Phospho-MYPT1 (Thr696) Polyclonal Antibody, Unconjugated (bs-3287R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3287R

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Rat Liver lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Rat Pancreas lysates probed with GSK-3 Beta Polyclonal Antibody, Unconjugated (bs-3148R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GSK-3 Beta(Thr390) Polyclonal Antibody(bs-3148R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3148R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2595R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-2520R

Applications: WB, ELISA, FCM

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2436R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse uterus tissue labeled with Anti-ABP\/SHBG Polyclonal Antibody, Unconjugated (bs-2410R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti ABP\/SHBG Polyclonal Antibody, Unconjugated (bs-2410R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 44kD. Observed band size: 44kD.
Lane 1: Human HepG2 cell lysates; Lane 2: Human U87MG cell lysates probed with SHBG Polyclonal Antibody, Unconjugated (bs-2410R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2410R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human A549 lysates probed with p-Smad3(Ser423) Polyclonal Antibody, Unconjugated (bs-2225R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad3 (Ser423) Polyclonal Antibody(bs-2225R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Heart lysates; Lane 2: Mouse Kidney lysates; Lane 3: Rat Cerebrum lysates probed with Smad3 Polyclonal Antibody, Unconjugated (bs-2225R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-Smad3 (Ser423)) polyclonal Antibody, Unconjugated (bs-2225R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2225R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1702R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ECE2 Polyclonal Antibody, Unconjugated (bs-1753R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: 293T cell lysates probed with ECE2 Polyclonal Antibody, Unconjugated (bs-1753R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1753R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human esophageal carcinoma tissue labeled with Anti-Cathepsin B Polyclonal Antibody, Unconjugated(bs-1500R) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Mouse Spleen lysates probed with Cathepsin B Polyclonal Antibody, Unconjugated (bs-1500R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1500R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical cancer labeled with Anti-ICAM-2\/CD102 Polyclonal Antibody, Unconjugated (bs-1258R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-ICAM-2\/CD102 Polyclonal Antibody, Unconjugated (bs-1258R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1258R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bcl2 (Ser87) Polyclonal Antibody(bs-12577R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bcl2 (Ser87) Polyclonal Antibody, Unconjugated (bs-12577R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bcl2 (Ser87) Polyclonal Antibody, Unconjugated (bs-12577R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bcl2 (Ser87) Polyclonal Antibody, Unconjugated (bs-12577R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-12577R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Lingo-1 Polyclonal Antibody, Unconjugated (bs-1177R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Lingo-1 Polyclonal Antibody, Unconjugated (bs-1177R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1177R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SYNPR Polyclonal Antibody, Unconjugated (bs-11367R) at 1:800 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Rat bulbus olfactorius lysates probed with Synaptoporin Polyclonal Antibody, Unconjugated (bs-11367R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-11367R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Antigen: bs-11204P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti PAX6 Polyclonal Antibody, Unconjugated (bs-11204R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Pax6 Polyclonal Antibody, Unconjugated (bs-11204R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse embryo tissue fixed in 4% PFA and paraffin embedded\\nCitrate buffer (0.01M, pH 6.0) boiling bath for 15min; Anti-PAX6 Polyclonal Antibody, Unconjugated(bs-11204R) 1:200, overnight at 4\u00b0C; followed up by Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI was used to stain the cell nuclei.\\n

Catalog #1: bs-11204R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

SH-SY5Y cells probed with Rabbit Anti-Dopamine Receptor D1 Polyclonal Antibody (bs-10610R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.
A549 lysates probed with Dopamine Receptor D1 Polyclonal Antibody, Unconjugated (bs-10610R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-10610R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: HepG2 lysates Lane 2: K562 lysates probed with Rabbit Anti-AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-10344R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse muscle lysates probed with Rabbit Anti-AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-10344R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-10344R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-10344R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-10344R

Applications: WB, ELISA, FCM, IHC-P

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti ERK1\/MAPK3 Polyclonal Antibody, Unconjugated (bs-1020R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colon labeled with Rabbit Anti ERK1\/MAPK3 Polyclonal Antibody, Unconjugated (bs-1020R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with Rabbit Anti-ERK1 Polyclonal Antibody, Unconjugated (bs-1020R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Image provided by One World Lab validation program. A431 cells probed with ERK1 Polyclonal Antibody (bs-1020R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-1020R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27\/HspB1\/HSP25 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-HSP27\/HspB1\/HSP25 Polyclonal Antibody, Unconjugated(bs-0730R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
L1 mouse brain lysates L2 mouse liver lysates probed with Anti HSP27\/HspB1\/HSP25 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 27kD. Observed band size:27kD.\\n
A431 cells probed with HSP27 Polyclonal Antibody, unconjugated (bs-0730R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0730R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0756R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-1011R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0709R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rabbit

Conjugate: FITC

Catalog #1: bs-0672R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0134R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bs-0445R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat skeletal muscle labeled with Anti-IRS2 Polyclonal Antibody (bs-0173R), Unconjugated at 1:200followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti IRS-2 Polyclonal Antibody, Unconjugated (bs-0173R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-0173R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0127R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: FITC

Catalog #1: bs-0293G

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-0533R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0217R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-0008R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-0522R-FITC

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-P38 MAPK (T180) Monoclonal Antibody, Unconjugated (bsm-63316R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-P38 MAPK (T180) Monoclonal Antibody, Unconjugated (bsm-63316R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-P38 MAPK (T180) Monoclonal Antibody, Unconjugated (bsm-63316R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-P38 MAPK (T180) Monoclonal Antibody, Unconjugated (bsm-63316R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-63316r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-LSP1 antibody (bsm-61255R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (LSP1) monoclonal Antibody, unconjugated (bsm-61255R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LSP1 Monoclonal Antibody, Unconjugated (bsm-61255R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat\r\n Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LSP1 Monoclonal Antibody, Unconjugated (bsm-61255R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61255r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Tissue: Human breast cancer\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60700R
Tissue: Human colon cancer\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60700R
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated(bsm-60700R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PTP1B Monoclonal Antibody, Unconjugated(bsm-60700R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-60700r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TTF1\/NKX2-1 Monoclonal Antibody, Unconjugated(bsm-60241R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TTF1\/NKX2-1 Monoclonal Antibody, Unconjugated(bsm-60241R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TTF1\/NKX2-1 Monoclonal Antibody, Unconjugated(bsm-60241R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with TTF1\/NKX2-1 monoclonal antibody, unconjugated (bsm-60241R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60241r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated