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scatter diagram showing peripheral blood lymphocytes stained with CD25. The cells were incubated with the antibody (bsm-33113M) for 30 min at 22\u00b0C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.
SR cells were\u00a0incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CD25 .Antibody(bsm-33113M)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bsm-33113m

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33075M-RBITC

Applications: FCM

Reactivity:

Conjugate: RBITC

Catalog #1: bsm-33075M-PerCP-Cy5.5

Applications: FCM

Reactivity:

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33075M-PerCP

Applications: FCM

Reactivity:

Conjugate: PerCP

Catalog #1: bsm-33075M-PE-Cy7

Applications: FCM

Reactivity:

Conjugate: PE-Cy7

Catalog #1: bsm-33075M-PE-Cy5.5

Applications: FCM

Reactivity:

Conjugate: PE-Cy5.5

Catalog #1: bsm-33075M-PE-Cy5

Applications: FCM

Reactivity:

Conjugate: PE-Cy5

Catalog #1: bsm-33075M-APC-Cy7

Applications: FCM

Reactivity:

Conjugate: APC-Cy7

Catalog #1: bsm-33075M-APC-Cy5.5

Applications: FCM

Reactivity:

Conjugate: APC-Cy5.5

Catalog #1: bsm-33075M-APC

Applications: FCM

Reactivity:

Conjugate: APC

Catalog #1: bsm-33075M-BF750

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33075M-BF680

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33075M-BF647

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33075M-BF594

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33075M-BF555

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33075M-BF488

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33075M-BF405

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33075M-BF350

Applications: FCM

Reactivity:

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33075M-PE

Applications: FCM

Reactivity:

Conjugate: PE

Catalog #1: bsm-33075M-FITC

Applications: FCM

Reactivity:

Conjugate: FITC

Catalog #1: bsm-33075M-Cy7

Applications: FCM

Reactivity:

Conjugate: Cy7

Catalog #1: bsm-33075M-Cy5.5

Applications: FCM

Reactivity:

Conjugate: Cy5.5

Catalog #1: bsm-33075M-Cy5

Applications: FCM

Reactivity:

Conjugate: Cy5

Catalog #1: bsm-33075M-Cy3

Applications: FCM

Reactivity:

Conjugate: Cy3

Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD2) Monoclonal Antibody, Unconjugated (bsm-33075M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD2) Monoclonal Antibody, Unconjugated (bsm-33075M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33075m

Applications: FCM

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0296P-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: RBITC

Catalog #1: bs-0296P-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0296P-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: PerCP

Catalog #1: bs-0296P-PE-Cy7

Applications: WB, FCM

Reactivity:

Conjugate: PE-Cy7

Catalog #1: bs-0296P-PE-Cy5.5

Applications: WB, FCM

Reactivity:

Conjugate: PE-Cy5.5

Catalog #1: bs-0296P-PE-Cy5

Applications: WB, FCM

Reactivity:

Conjugate: PE-Cy5

Catalog #1: bs-0296P-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC-Cy7

Catalog #1: bs-0296P-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC-Cy5.5

Catalog #1: bs-0296P-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC

Catalog #1: bs-0296P-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0296P-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0296P-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0296P-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0296P-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0296P-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0296P-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-30038m-rbitc

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-30038m-percp-Cy5.5

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-30038m-percp

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-30038m-pe-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-30038m-apc-cy7

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-30038m-apc-Cy5.5

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-30038m-bf750

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-750