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Catalog #1: bsm-30093M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30093M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30093M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33085m-cy7

Applications: WB, IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33085m-pe

Applications: WB, IF

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-33085m-cy3

Applications: WB, IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33085m-cy5

Applications: WB, IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33085m-fitc

Applications: WB, IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-33062M-PE

Applications: WB, FCM, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33085m-Cy5.5

Applications: WB, IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-33033m-cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy3

Catalog #1: bsm-33033m-pe

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE

Catalog #1: bsm-33033m-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy5.5

Catalog #1: bsm-33033m-cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy7

Catalog #1: bsm-33033m-cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy5

Catalog #1: bsm-30105M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33062M-FITC

Applications: WB, FCM, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33062M-Cy7

Applications: WB, FCM, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33062M-Cy5

Applications: WB, FCM, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33062M-Cy5.5

Applications: WB, FCM, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33062M-Cy3

Applications: WB, FCM, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD45 Monoclonal Antibody, Unconjugated (bsm-33052M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD45 Monoclonal Antibody, Unconjugated (bsm-33052M) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human Molt-4 cell lysates; Lane 2: Human K562 cell lysates probed with CD45 Monoclonal Antibody, Unconjugated (bsm-33052M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U937 cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CD45 Monoclonal Antibody(bsm-33052M)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bsm-33052m

Applications: WB, ELISA, FCM, IHC-P, IHC-F

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0345M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33187m-Cy5.5

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-0296P-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5.5

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C-erbB-2\/HER2 Monoclonal Antibody, Unconjugated (bsm-33085M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C-erbB-2\/HER2 Monoclonal Antibody, Unconjugated (bsm-33085M) at 1:400 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with C-erbB-2\/HER2 monoclonal antibody, unconjugated (bsm-33085M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33085m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33187m-cy7

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33187m-cy5

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33187m-cy3

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Lane 1: A431; Lane 2: HeLa lysate at 20 \u00b5g per lane, probed with bsm-51104M ACTA1 (337CT30.10.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ACTA1 (337CT30.10.1) Monoclonal Antibody (bsm-51104M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: K562; Lane 2: CEM lysate at 20 \u00b5g per lane, probed with bsm-51104M ACTA1 (337CT30.10.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-51104M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51095M

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: HepG2 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: NCI-H460 Cell lysates; probed with ALDH1A1 (2C2) Monoclonal Antibody, unconjugated (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: NCI-H460 Cells; 15 ug of protein lysate loaded; Probed with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:25 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin (red). Cells were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X100\/PBS.

Catalog #1: bsm-51032M

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51027M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A549 Cell lysates; Lane 2: SK-BR-3 Cell lysates; Lane 3: C2C12 Cell lysates; probed with CAPN2 (1C1) Monoclonal Antibody, unconjugated (bsm-51429M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CAPN2 (1C1) Monoclonal Antibody (bsm-51429M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Flow cytometric analysis of U-87 MG cells using bsm-51429M CAPN2 Antibody (green) compared to mouse IgG2b isotype control (blue). bsm-51429M was diluted at 1:100 dilution. Alexa Fluor\u00ae 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Catalog #1: bsm-51429M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: rat liver lysates; Lane 2: NIH\/3T3 Cell lysates; Lane 3: MCF-7 Cell lysates; Lane 4: mouse liver lysates; Lane 5: C6 Cell lysates;probed with VCP (4C4) Monoclonal Antibody, unconjugated (bsm-51420M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Fluorescent image of U251 cells stained with VCP (4C4) Antibody (bsm-51420M) at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor\u00ae 555 conjugated with Phalloidin (red).

Catalog #1: bsm-51420M

Applications: WB, FCM, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7 Cell lysates; Lane 2: A549 Cell lysates; Lane 3: A431 Cell lysates; probed with CAPN1 (6C5) Monoclonal Antibody, unconjugated (bsm-51419M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CAPN1 (6C5) Monoclonal Antibody (bsm-51419M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Hela cells probed with using CAPN1 (6C5) Monoclonal Antibody bsm-51419M at 1:25 for 60 minutes followed by A488 secondary antibody at 1:400 (green), compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51419M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: NCI-H292 Cell lysates; Lane 2: A549 Cell lysates; Lane 3: HepG2 Cell lysates; probed with ALDH2 (138CT22.3.8) Monoclonal Antibody, unconjugated (bsm-51417M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ANTIBODY Monoclonal Antibody (bsm-51417M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51417M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with YY1 (3C6) Monoclonal Antibody (bsm-51412M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U-251 cell lysates; probed with YY1 (3C6) Polyclonal Antibody, unconjugated (bsm-51412M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Monkey brain tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with YY1 (3C6) Monoclonal Antibody, Unconjugated (bsm-51412M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51412M

Applications: WB, FCM, IHC-P

Reactivity: Human, Rat, Monkey

Conjugate: Unconjugated

Lane 1: Daudi Cell lysates; Lane 2: Raji Cell lysates; probed with BCL10 (5C2) Monoclonal Antibody, unconjugated (bsm-51398M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BCL10 (5C2) Monoclonal Antibody (bsm-51398M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Hela cells probed with using BCL10 (5C2) Monoclonal Antibody (green) unconjugated bsm-51398M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG (blue).
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with BCL10 (5C2) Monoclonal Antibody, Unconjugated (bsm-51398M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51398M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
A431 cells probed with using MYO18B (3C5) Monoclonal Antibody (green) unconjugated bsm-51376M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG2b (blue).
Recombinant protein lysates probed with MYO18B (3C5) Polyclonal Antibody, unconjugated (bsm-51376M) at 1:16000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51376M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TERF2IP (4C8) Monoclonal Antibody (bsm-51368M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: HepG2 cell lysates; Lane 2: A431 cell lysates; probed with TERF2IP (4C8) Polyclonal Antibody, unconjugated (bsm-51368M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51368M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human lung lysates; probed with RYK (1C1) Monoclonal Antibody, unconjugated (bsm-51365M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
A431 cells probed with RYK (1C1) Monoclonal Antibody (green) unconjugated bsm-51365M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 (green), compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51365M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: A431 Cell lysates; Lane 3: PC-12 Cell lysates; Lane 4: COS-7 Cell lysates; Lane 5: NIH\/3T3 Cell lysates; probed with MP2K1 (8C8) Monoclonal Antibody, unconjugated (bsm-51364M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells probed with using MP2K1 (8C8) Monoclonal Antibody (green) unconjugated bsm-51364M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51364M

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: K562 Cell lysates; Lane 3: Daudi Cell lysates; probed with PLIN3 (1C4) Monoclonal Antibody, unconjugated (bsm-51361M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HUVEC cells probed with using PLIN3 (1C4) Monoclonal Antibody (green) unconjugated bsm-51361M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).
Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS cells; Antibody incubation with PLIN3 (1C4) Monoclonal Antibody, Unconjugated (bsm-51361M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). The nuclear counter stain is DAPI (blue).

Catalog #1: bsm-51361M

Applications: WB, FCM, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Jurkat Cell lysates; Lane 2: Ramos Cell lysates; Lane 3: HT-1080 Cell lysates; probed with GARS (1C6) Monoclonal Antibody, unconjugated (bsm-51350M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells; antibody incubation with GARS (1C6) Monoclonal Antibody, Unconjugated (bsm-51350M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Hela cells probed with using GARS (1C6) Monoclonal Antibody (green) unconjugated bsm-51350M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51350M

Applications: WB, FCM, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Raji Cell lysates; probed with RASSF2 (9C9) Monoclonal Antibody, unconjugated (bsm-51348M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells probed with using RASSF2 (9C9) Monoclonal Antibody bsm-51348M at 1:25 for 30 minutes followed by A488 secondary antibody at 1:200 (green) compared to an isotype control of mouse IgG2a (blue).

Catalog #1: bsm-51348M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: PC-12 cell lysate (20\u00b5g per lane) probed with bsm-51346M AKT2 (1623CT791.157.67.66) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa cells labeled with AKT2 (16C6) Monoclonal Antibody at 1:25 dilution, followed by goat anti-mouse IgG secondary antibody (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1\/100 dilution (red). Nuclear counter stain is DAPI (blue).

Catalog #1: bsm-51346M

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: MOLT-4 Cell lysates; probed with ETS1 (1C1) Monoclonal Antibody, unconjugated (bsm-51344M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cells probed with using ETS1 (1C1) Monoclonal Antibody (green) unconjugated bsm-51344M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG2b (blue).

Catalog #1: bsm-51344M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated