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Catalog #1: bsm-33024m-pe-Cy5.5

Applications: WB, FCM

Reactivity: Species independent

Conjugate: PE-Cy5.5

Catalog #1: bsm-33024m-percp-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33024m-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy5.5

Catalog #1: bsm-33002m-percp-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33002m-apc-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: APC-Cy5.5

Catalog #1: bsm-33002m-pe-Cy5.5

Applications: WB, FCM

Reactivity: Species independent

Conjugate: PE-Cy5.5

Catalog #1: bsm-33002m-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy5.5

Catalog #1: bsm-30094m-percp-cy7

Applications: FCM

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-30090m-percp-cy7

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-30090m-pe-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-30090m-bf647

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-30073m-percp-cy7

Applications: FCM

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-25816m-apc-Cy5.5

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-30090m-bf680

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-25816m-pe-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-30090m-Cy5.5

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30073m-bf680

Applications: FCM

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-30090m-apc-Cy5.5

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-25816m-percp-Cy5.5

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-30038m-percp-cy7

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-25816m-Cy5.5

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30073m-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30073m-pe-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-30073m-bf647

Applications: FCM

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-30073m-apc-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-25816m-percp-cy7

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-10832m-pe-cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-10832m-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-10832m-pe-cy55

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-10832m-percp-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-10832m-apc-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-10832m-pe-cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-41304m-pe-Cy5.5

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-41304m-percp-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-41304m-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-41304m-apc-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-63326M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-63326M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-63326M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-63326M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-63326m

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60742m

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-HDAC3 antibody (bsm-60265M,1:100); Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HDAC3) monoclonal Antibody, unconjugated (bsm-60625M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-60265m

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with ERK2 monoclonal antibody, unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-ERK2 antibody (bsm-51555M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-51555m

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BCL10 Monoclonal Antibody(bsm-51578M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BCL10 Monoclonal Antibody, Unconjugated(bsm-51578M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BCL10 Monoclonal Antibody, Unconjugated(bsm-51578M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BCL10 Monoclonal Antibody, Unconjugated(bsm-51578M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51578m

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-CD38 antibody (bsm-43703M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (CD38) monoclonal Antibody, unconjugated (bsm-43703M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with CD38 monoclonal antibody, unconjugated (bsm-43703M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD38 Monoclonal Antibody, Unconjugated (bsm-43703M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-43703m

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43685m

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

4% Paraformaldehyde-fixed Jurkat (H) cell; Antibody incubation with (CD31) monoclonal Antibody, unconjugated (bsm-43666M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The EA.hy926(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-CD31 antibody (bsm-43666M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488(bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Jurkat(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-CD31 antibody (bsm-43666M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD31 Monoclonal Antibody, Unconjugated (bsm-43666M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-43666m

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with TFRC monoclonal antibody, unconjugated (bsm-43165M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Mouse Anti-TFRC antibody (bsm-41365M): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Mouse IgG (bs-0296P). Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-40296G-FITC): 1 \u03bcg\/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (TFRC) monoclonal Antibody, Unconjugated (bsm-43165M) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-43165m

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Recombinant human FOXP3 protein, N-His(bs-42173P) probed with FOXP3 Monoclonal Antibody, Unconjugated (bsm-42173M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
The MJ (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-FOXP3 antibody (bsm-42173M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-42173m

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hep-G2 (H) cells were treated with or without CoCl2 (500uM) for 6 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with HIF-1 Alpha monoclonal antibody, unconjugated (bsm-33428M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HepG2\uff08treated with 1mM CoCl2 for 24 hours\uff09 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1a) monoclonal Antibody, unconjugated (bsm-33428M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Hela\uff08treated with 1mM CoCl2 for 24 hours\uff09 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIF-1a) monoclonal Antibody, unconjugated (bsm-33428M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (treated with 500uM CoCl2 for 6 hours) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-HIF-1 Alpha antibody (bsm-33428M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-33428m

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-ERK1\/2 antibody (bsm-33232M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-33232m

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Unconjugated