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Catalog #1: bsm-30038m-cy5

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30038m-cy3

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (human CD44) Monoclonal Antibody, Unconjugated (bsm-30038M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (human CD44) polyclonal Antibody, Unconjugated (bsm-30038M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.PBS instead of the primary antibody was used as the blank control.
Hela cells were\u00a0incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CD44 .Antibody(bsm-30038M)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (human CD44) Monoclonal Antibody, Unconjugated (bsm-30038M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-30038m

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30155a-pe-cy5-25t

Applications: FCM

Reactivity: Mouse

Conjugate: PE-Cy5

Catalog #1: bsm-30155a-pe-100t

Applications: FCM

Reactivity: Mouse

Conjugate: PE

Catalog #1: bsm-30155a-fitc-100t

Applications: FCM

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bsm-30155a-apc-100t

Applications: FCM

Reactivity: Mouse

Conjugate: APC

Catalog #1: bsm-30098M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with YY1 (3C6) Monoclonal Antibody (bsm-51412M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U-251 cell lysates; probed with YY1 (3C6) Polyclonal Antibody, unconjugated (bsm-51412M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Monkey brain tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with YY1 (3C6) Monoclonal Antibody, Unconjugated (bsm-51412M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51412M

Applications: WB, FCM, IHC-P

Reactivity: Human, Rat, Monkey

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: K562 Cell lysates; Lane 3: Daudi Cell lysates; probed with PLIN3 (1C4) Monoclonal Antibody, unconjugated (bsm-51361M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HUVEC cells probed with using PLIN3 (1C4) Monoclonal Antibody (green) unconjugated bsm-51361M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG1 (blue).
Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS cells; Antibody incubation with PLIN3 (1C4) Monoclonal Antibody, Unconjugated (bsm-51361M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary (green). The nuclear counter stain is DAPI (blue).

Catalog #1: bsm-51361M

Applications: WB, FCM, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Raji Cell lysates; probed with RASSF2 (9C9) Monoclonal Antibody, unconjugated (bsm-51348M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells probed with using RASSF2 (9C9) Monoclonal Antibody bsm-51348M at 1:25 for 30 minutes followed by A488 secondary antibody at 1:200 (green) compared to an isotype control of mouse IgG2a (blue).

Catalog #1: bsm-51348M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

ICC analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with Zap70 (4C2) Monoclonal Antibody (bsm-51298M) at 1:25 dilution, followed by secondary antibody incubation. Immunofluorescence image shows cytoplasm staining of Zap70 (green), actin (red), and nuclear counter stain with DAPI (blue).
Lane 1: Jurkat Cell lysates; Lane 2: MOLT-4 Cell lysates; Lane 3: mouse thymus Cell lysates; probed with Zap70 (4C2) Monoclonal Antibody, unconjugated (bsm-51298M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Jurkat cells probed with using Zap70 (4C2) Monoclonal Antibody, bsm-51298M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG2a (blue).

Catalog #1: bsm-51298M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated