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Catalog #1: bs-3125R-Cy5

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy5

MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-Cyclin E1 (Thr77) Polyclonal Antibody(bs-3125R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cyclin E (Thr77) Polyclonal Antibody(bs-3125R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3125R

Applications: WB, ELISA, FCM, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1735R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-1735R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-1735R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-1735R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-1735R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-1735R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12913R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-12913R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-12913R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-12913R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-12913R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-12913R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10441R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-10441R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-10441R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-10441R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-10441R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Formalin-fixed and paraffin embedded rat brain labeled with Anti-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) Polyclonal Antibody, Unconjugated (bs-10441R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) ) Polyclonal Antibody, Unconjugated (bs-10441R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) ) Polyclonal Antibody, Unconjugated (bs-10441R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) ) Polyclonal Antibody, Unconjugated (bs-10441R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-10441R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-19452R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-9764R-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-8442R-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bs-6582R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-6581R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bs-6031R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Monkey

Conjugate: Cy5.5

Catalog #1: bs-5703R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5677R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bs-5669R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-5661R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bs-5660R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5628R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5617R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bs-5596R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5590R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5577R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5575R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-5540R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-5515R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-5514R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bs-5511R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5509R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5502R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-5474R-Cy5.5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-5473R-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-5470R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-5463R-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5459R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5