CD8 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0648r-bf647 |
| Product Name | CD8 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
FCM
IF(IHC-P)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse CD8 |
| Immunogen Range | 51-150/247 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 12525 |
| Swiss Prot | P01731 |
| Subcellular location | Cell membrane |
| Synonyms | Ly-2; Ly-B; Ly-35; Lyt-2; BB154331; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD8a; Lyt2 |
| Background | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
| Application Dilution | |
| FCM | 1:50 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is CD8 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0648R-BF647) validated for, and at what dilution?
bs-0648R-BF647 is validated for FCM (1:50); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD8 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0648R-BF647) react with?
Tested reactivity: Human, Mouse.
How should bs-0648R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD8 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0648R-BF647)?
KLH conjugated synthetic peptide derived from mouse CD8 Immunogen range: 51-150/247.
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-0648R-BF647)?
AbBy Fluor-647 on bs-0648R-BF647: excitation / emission 650nm/665nm.