Nephrin Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10233r-bf405 |
| Product Name | Nephrin Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
FCM
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Nephrin |
| Immunogen Range | 451-550/1241 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4868 |
| Swiss Prot | O60500 |
| Subcellular location | Extracellular matrix |
| Synonyms | CNF; NPHN; nephrin; Renal glomerulus-specific cell adhesion receptor; NPHS1 |
| Background | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). |
| Application Dilution | |
| WB | 1ug/Test |
| FCM | 1:500-2000 |
| IF | 1:100-500 |
| Flow-Cyt | 1:100-500 |
Frequently asked questions
Which applications is Nephrin Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10233R-BF405) validated for, and at what dilution?
bs-10233R-BF405 is validated for WB (1ug/Test); FCM (1:500-2000); IF (1:100-500); Flow-Cyt (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nephrin Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10233R-BF405) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Rabbit.
How should bs-10233R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nephrin Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10233R-BF405)?
KLH conjugated synthetic peptide derived from human Nephrin Immunogen range: 451-550/1241.
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-10233R-BF405)?
AbBy Fluor-405 on bs-10233R-BF405: excitation / emission 401nm/421nm.