Beta tubulin Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4511R-BF594 |
| Product Name | Beta tubulin Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human tubulin Beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 203068 |
| Swiss Prot | P07437 |
| Subcellular location | Cytoplasm |
| Synonyms | M4; TUBB1; TUBB5; CDCBM6; OK/SW-cl.56; Tubulin beta chain; Tubulin beta-5 chain; TUBB |
| Background | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Beta tubulin Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-4511R-BF594) validated for, and at what dilution?
bs-4511R-BF594 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Beta tubulin Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-4511R-BF594) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-4511R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Beta tubulin Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-4511R-BF594)?
KLH conjugated synthetic peptide derived from human tubulin Beta
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-4511R-BF594)?
AbBy Fluor-594 on bs-4511R-BF594: excitation / emission 590nm/617nm.