Glucose 6 Phosphate Dehydrogenase Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with Glucose 6 Phosphate Dehydrogenase monoclonal antibody, unconjugated (bsm-61479R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Glucose 6 Phosphate Dehydrogenase monoclonal antibody, unconjugated (bsm-61479R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-61479r
Product Name	Glucose 6 Phosphate Dehydrogenase Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human G6PD
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2539
Swiss Prot	P11413
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	G6PD; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; Glucose 6 phosphate dehydrogenase; Glucose 6 phosphate dehydrogenase, G6PD; MET19; POS10; Zwf1p.
Background	Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF
Flow-Cyt	1ug/Test
ICC/IF	1:50-100