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Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-Matriptase Polyclonal Antibody (bs-0531R), Unconjugated at 1:100 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-Matriptase Polyclonal Antibody(bs-0531R), Unconjugated at 1:100 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Jurkat lysates probed with Matriptase Polyclonal Antibody, Unconjugated (bs-0531R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Matriptase) polyclonal Antibody, Unconjugated (bs-0531R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0531R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4053R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-4053R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-4053R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-4122R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4122R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-4122R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-7135R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7135R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-7135R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-5915R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-5915R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-5915R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Western blot was performed on nuclear extracts from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against MBD2 (bs-53058R), diluted 1:500 in BSA\/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against MBD2 (bs-53058R). By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:185,000.

Catalog #1: bs-53058R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-51058R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human MBD4 (bs-53050R) in antigen-coated wells. The wells were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:15,000.
(Left) U2OS cells were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MBD4 (bs-53050R) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. (Right) Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 \u03bcg) with the antibody against MBD4 (bs-53050R), diluted 1:2,000 in TBST containing 3% milk powder.

Catalog #1: bs-53050R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51050R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-5721R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: Mouse stomach lysates probed with Ghrelin O acyltransferase Antibody, Unconjugated (bs-13355R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MBOAT4) Polyclonal Antibody, Unconjugated (bs-13355R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-13355r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13355R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-13355R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-MBP Polyclonal Antibody (bs-0380R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C DAPI(5ug\/ml, blue) was used to stain the cell nuclei
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-MBP Polyclonal Antibody (bs-0380R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MBP Antibody(bs-0380R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (bs-0380R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0380r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0380R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0380R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Antigen: bs-2967P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm.
Lane 1: MBP-tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33131P) probed with Maltose Binding Protein\/MBP Polyclonal Antibody, Unconjugated (bs-2967R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MBP-Tagged Fusion Protein Overexpression E.coli Lysate (bs- 41233P) probed with MBP tag Polyclonal Antibody, Unconjugated (bs-2967R) at 1:200000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2967R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bs-2967R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bs-2967R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: HRP

Lane 1:mouse kidney lysates Lane 2: mouse brain lysates probed with Anti-MC-1R\/MC1 Receptor Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 35kD. Observed band size: 35kD.
paraformaldehyde-fixed, paraffin embedded human melanoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-MC-1R Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Human 293T Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min. Cells were stained with MC-1R\/MC1 Polyclonal Antibody, Alexa Fluor 488 Conjugated (bs-1419R-A488) at 1:100 for 30 min on ice. Primary Antibody staining (green) is compared to unstained cells (dark blue), and isotype control (orange).
Lane 1: MOLT-4 Cells; Probed with MC-1R\/MC1 Polyclonal Antibody, Unconjugated (bs-1419R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-1419R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-23517R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1419R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-23517R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-1419R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-23517R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-7558R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7558R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-7558R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

MCF-7 cell lysates probed with Rabbit Anti-MC4 Receptor Polyclonal Antibody, Unconjugated (bs-11417R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
RSC96 probed with MC4 Receptor Polyclonal Antibody, Unconjugated (bs-11417R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Embryo lysates probed with MC4 Receptor Polyclonal Antibody, Unconjugated (bs-11417R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11417R

Applications: ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11417R-Biotin

Applications: ELISA, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-11417R-HRP

Applications: ELISA, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Mouse spleen cells (2x10^6) probed with Rabbit Anti-MC5 Receptor\/MC5-R Polyclonal Antibody (bs-11418R) at 1:100 for 40 minutes at room temperature followed by Goat Anti Rabbit IgG (H+L) Secondary Antibody, FITC Conjugated
Formalin-fixed and paraffin embedded mouse placenta labeled with Rabbit Anti-MC5 Receptor\/MC5-R Polyclonal Antibody, Unconjugated (bs-11418R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-11418R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11418R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-11418R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-6072R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6072R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-6072R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-7616R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7616R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-7616R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP