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Catalog #1: bs-0154R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0154R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0154R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-0154R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0154R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0154R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-0154R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-0154R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-0154R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-0154R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-0154R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0154R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Flow cytometric analysis of SH-sy5y cells with GAP43 (8H2) Monoclonal Antibody (bsm-52565R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with GAP43 (8H2) Monoclonal Antibody (bsm-52565R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAP43 Monoclonal Antibody, Unconjugated(bsm-52565R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAP43 Monoclonal Antibody, Unconjugated(bsm-52565R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52565r

Applications: WB, IHC-P, IHC-F, IP, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33033m

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

Catalog #1: bsm-33033m-bf350-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33033m-bf405-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33033m-bf488-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33033m-bf555-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33033m-bf594-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33033m-bf647-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33033m-bf680-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33033m-bf750-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33033m-apc-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC

Catalog #1: bsm-33033m-apc-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC-Cy5.5

Catalog #1: bsm-33033m-apc-cy7-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC-Cy7

Catalog #1: bsm-33033m-cy3-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy3

Catalog #1: bsm-33033m-cy5-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy5

Catalog #1: bsm-33033m-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy5.5

Catalog #1: bsm-33033m-cy7-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy7

Catalog #1: bsm-33033m-fitc-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: FITC

Catalog #1: bsm-33033m-pe-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE

Catalog #1: bsm-33033m-pe-cy5-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy5

Catalog #1: bsm-33033m-pe-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy5.5

Catalog #1: bsm-33033m-pe-cy7-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy7

Catalog #1: bsm-33033m-percp-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PerCP

Catalog #1: bsm-33033m-percp-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33033m-percp-cy7-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PerCP-Cy7

Catalog #1: bsm-33033m-rbitc-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: RBITC

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-GAPDH Polyclonal Antibody, Unconjugated (bs-0459R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
This image was generously provided by Loyda M. Mel\u00e9ndez at the University of Puerto Rico Medical Sciences Campus.\\nA total of 30ug of monocyte-derived macrophage lysates were reduced and denatured in 1X Laemli Buffer with beta-mercaptoethanol. Samples were loaded into a TGX 4-20% gel, at 150V for 1 hour. Proteins were transferred to a PVDF membrane and probed for proteins of interest. The membrane was re-probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) in 5% milk at 1:250 dilution overnight at 4\u00b0C. The next day, the membrane was washed with TBS-T and incubated with goat anti-rabbit HRP conjugated secondary antibody for 1 hour at room temperature. The membrane was exposed for 18 seconds.\\n
MCF-7 cell lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.
Mouse muscle lysates probed with Rabbit Anti-GAPDH Polyclonal Antibody (bs-0459R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0459R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (3B5) Monoclonal Antibody, Unconjugated (bsm-52262R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52262r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52262r-bf350-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-52262r-bf405-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-52262r-bf488-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-52262r-bf555-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-52262r-bf594-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-52262r-bf647-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-52262r-bf680-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-52262r-bf750-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750