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Catalog #1: bsm-62306r-apc-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-62306r-apc-Cy5.5-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-62306r-apc-cy7-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-62306r-cy3-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-62306r-cy5-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-62306r-Cy5.5-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-62306r-cy7-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-62306r-fitc-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-62306r-pe-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-62306r-pe-cy5-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-62306r-pe-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-62306r-pe-cy7-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-62306r-percp-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-62306r-percp-Cy5.5-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-62306r-percp-cy7-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-62306r-rbitc-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Estrogen receptor alpha) Monoclonal Antibody, Unconjugated (bsm-60803R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human endometrium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Estrogen receptor alpha) Monoclonal Antibody, Unconjugated (bsm-60803R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with Estrogen receptor alpha monoclonal antibody, unconjugated (bsm-60803R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Estrogen receptor alpha antibody (bsm-60803R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-60803r-100ul

Applications: FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7 Lane 2: DU145 lysates probed with Anti-Estrogen Receptor alpha Polyclonal Antibody, Unconjugated (bs-0122R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Tissue\/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0122R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Rat Liver lysates; Lane 4: Human MOLT-4 cell lysates; Lane 5: Human Raji cell lysates; Lane 6: Human Hela cell lysates; Lane 7: Human A549 cell lysates probed with Estrogen Receptor alpha Polyclonal Antibody, Unconjugated (bs-0122R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0122R

Applications: WB, ELISA, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Rat brain lysates probed with Anti ER\u03b1\/ESR1 Polyclonal Antibody, Unconjugated (bs-0725R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 67kD. Observed band size:67kD.
MCF-7 cells probed with Estrogen Receptor alpha Antibody, unconjugated (bs-0725R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Estrogen Receptor alpha Polyclonal Antibody, Unconjugated (bs-0725R) at 1:200 overnight at 4\u00b0C, DAB staining.
Tissue\/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0725R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0725R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial tissue labeled with Anti Estrogen Receptor alpha Polyclonal Antibody (bs-2098R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
MCF-7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Estrogen Receptor alpha Polyclonal Antibody(bs-2098R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Estrogen Receptor alpha Polyclonal Antibody, Unconjugated (bs-2098R) at 1:200 overnight at 4\u00b0C, DAB staining.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-2098R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2098R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0122R-BF350

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-2098R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-0725R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-0122R-BF405

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-2098R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-0725R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-0122R-BF488

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-2098R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-0725R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-0122R-BF555

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-2098R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-0725R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-0122R-BF594

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-2098R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-0725R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-0122R-BF647

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-2098R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-0725R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-0122R-BF680

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-2098R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-0725R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-0122R-BF750

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-2098R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-0725R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-0122R-APC

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: APC

Catalog #1: bs-2098R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: APC

Catalog #1: bs-0122R-APC-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-2098R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: APC-Cy5.5