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Catalog #1: bs-10176R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-10176R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: RBITC

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-AKT1S1 antibody (bsm-61149R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (AKT1S1) monoclonal Antibody, unconjugated (bsm-61149R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with AKT1S1 Monoclonal Antibody, Unconjugated (bsm-61149R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with AKT1S1 Monoclonal Antibody, Unconjugated (bsm-61149R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61149r-100ul

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-61149r-bf350-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61149r-bf405-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61149r-bf488-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61149r-bf555-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61149r-bf594-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61149r-bf647-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61149r-bf680-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61149r-bf750-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61149r-apc-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bsm-61149r-apc-Cy5.5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bsm-61149r-apc-cy7-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-61149r-cy3-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-61149r-cy5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-61149r-Cy5.5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-61149r-cy7-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-61149r-fitc-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-61149r-pe-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-61149r-percp-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bsm-61149r-percp-Cy5.5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-61149r-percp-cy7-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy7

Catalog #1: bsm-61149r-rbitc-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: RBITC

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: PC-12 cell lysate (20\u00b5g per lane) probed with bsm-51346M AKT2 (1623CT791.157.67.66) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa cells labeled with AKT2 (16C6) Monoclonal Antibody at 1:25 dilution, followed by goat anti-mouse IgG secondary antibody (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin at 1\/100 dilution (red). Nuclear counter stain is DAPI (blue).

Catalog #1: bsm-51346M

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Mouse Cerebrum lysates probed with AKT2 Polyclonal Antibody, unconjugated (bs-2056R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT2 Polyclonal Antibody, Unconjugated (bs-2056R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AKT2 ) Polyclonal Antibody, Unconjugated (bs-2056R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AKT2 ) Polyclonal Antibody, Unconjugated (bs-2056R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2056R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT2 Polyclonal Antibody, Unconjugated (bs-10724R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT2 Polyclonal Antibody, Unconjugated (bs-10724R) at 1:400 overnight at 4\u00b0C, DAB staining.
Mouse lung lysates probed with AKT2 Polyclonal Antibody, Unconjugated (bs-10724R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat lung lysates probed with AKT2 Polyclonal Antibody, Unconjugated (bs-10724R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10724R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2056R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-10724R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-2056R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-10724R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-2056R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-10724R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-2056R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-10724R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-2056R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-10724R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-2056R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-10724R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-2056R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-10724R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-2056R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-10724R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-2056R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-10724R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: APC

Catalog #1: bs-2056R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-10724R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-2056R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7