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Catalog #1: bs-3559R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0251R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0251R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0251R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-RAR Beta Polyclonal Antibody (bs-0516), Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining
L1 human colon carcinoma lysates L2 rat heart lysates probed with Anti IL-2R gamma\/CD132 Polyclonal Antibody, Unconjugated (bs-2545R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 41kD. Observed band size:41kD.\\n
Image provided by One World Lab validation program. HEK293 cells probed with RAR Beta Polyclonal Antibody (bs-0516R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n
SH-SY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RAR Beta Polyclonal Antibody(bs-0516R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0516R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0516R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0516R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-1515R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with Ras monoclonal antibody, unconjugated (bsm-62921R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ras Monoclonal Antibody, Unconjugated (bsm-62921R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ras Monoclonal Antibody, Unconjugated (bsm-62921R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ras Monoclonal Antibody, Unconjugated (bsm-62921R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62921r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62921r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-62921r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-13280R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-13280R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-13280R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

25 ug total protein per lane of various lysates (see on figure) probed with RASA1 monoclonal antibody, unconjugated (bsm-62507R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The U-87MG (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-RASA1 antibody (bsm-62507R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RASA1 Monoclonal Antibody, Unconjugated (bsm-62507R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62507r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62507r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-62507r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-6171R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6171R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-6171R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-RASA3 Polyclonal Antibody, Unconjugated (bs-5954R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat tongue labeled with Anti-RASA3 Polyclonal Antibody, Unconjugated (bs-5954R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-5954R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-5954R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-5954R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-6088R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-6088R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-6088R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Lane 1: A549 cell lysates; Lane 2: Siha cell lysates probed with RASD2 Polyclonal Antibody, Unconjugated (bs-7006R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Human U87MG cell lysates probed with RASD2 Polyclonal Antibody, Unconjugated (bs-7006R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7006R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7006R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-7006R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-19731R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-19731R-Biotin

Applications: IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-19731R-HRP

Applications: IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-5722R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-5722R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-5722R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Anti-RASSF1A Polyclonal Antibody, Unconjugated (bs-1234R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-RASSF1A Polyclonal Antibody, Unconjugated (bs-1234R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded human oral squamous cell labeled with Anti-RASSF1A Polyclonal Antibody, Unconjugated(bs-1234R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Formalin-fixed and paraffin-embedded human oral squamous cell labeled with Anti-RASSF1A Polyclonal Antibody, Unconjugated(bs-1234R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FTIC)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-1234R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1234R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1234R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RASSF1A Monoclonal Antibody, Unconjugated (bsm-61576R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RASSF1A Monoclonal Antibody, Unconjugated (bsm-61576R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RASSF1A Monoclonal Antibody, Unconjugated (bsm-61576R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RASSF1A Monoclonal Antibody, Unconjugated (bsm-61576R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61576r-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61576r-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-61576r-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Anti-RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse brain lysates Lane 2: mouse kidney lysates probed with Anti RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 36kD. Observed band size: 36kD.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF2 Polyclonal Antibody, Unconjugated (bs-2939R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2939R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2939R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2939R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Lane 1:A549 lysates probed with Rabbit Anti-RASSF3 Polyclonal Antibody, Unconjugated (bs-6078R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Stomach lysates probed with RASSF3 Polyclonal Antibody, Unconjugated (bs-6078R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF3 Polyclonal Antibody, Unconjugated (bs-6078R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RASSF3 Polyclonal Antibody, Unconjugated (bs-6078R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-6078R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-6078R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-6078R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-11168R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated