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Catalog #1: bs-8031R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-8031R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-0617R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with cofilin Polyclonal Antibody, Unconjugated (bs-2759R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with cofilin Polyclonal Antibody, Unconjugated (bs-2759R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2759R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2759R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-2759R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-6647R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-6647R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-6647R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-5881R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-5881R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-5881R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-2160R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2160R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-2160R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-5044R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-5044R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-5044R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Formalin-fixed and paraffin-embedded rat skin tissue labeled with Anti-Collagen V Polyclonal Antibody, Unconjugated (bs-0552R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Anti-Collagen V Polyclonal Antibody, Unconjugated (bs-0552R) 1:200 followed by conjugation to the secondary PE antibody and DAB staining
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Skin tissue lysates; Lane 3: Mouse Uterus tissue lysates; Lane 4: Rat Uterus tissue lysates; Lane 5: Human A549 cell lysates probed with COL5A1 Polyclonal Antibody, Unconjugated (bs-0552R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0552R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8033R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-8033R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-8033R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-8032R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-8032R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-8032R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Formalin-fixed and paraffin embedded rabbit meniscus tissue labeled Anti-Collagen II Polyclonal Antibody, Unconjugated (bs-0709R) at 1:200, followed by conjugation to the secondary Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C. and DAPI
Lane 1: Human 293T lysates; Lane 2: Human A549 lysates; Lane 3: Human MCF-7 lysates; Lane 4: Human HepG2 lysates; Lane 5: Mouse Placenta lysates; Lane 6: Human Mg63 lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-0709R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0709R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0709R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rabbit

Conjugate: Biotin

Catalog #1: bs-0709R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rabbit

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Collagen III Polyclonal Antibody, Unconjugated (bs-0948R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela lysates probed with Collagen III Polyclonal Antibody, Unconjugated (bs-0948R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with Collagen III polyclonal antibody, unconjugated (bs-0948R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed RD (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Collagen III) polyclonal Antibody, unconjugated (bs-0948R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bs-0948r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0948R-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0948R-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin-embedded Rat Skeletal Muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (bsm-33400M) at 1:400 overnight at 4\u00b0C, followed by a secondary antibody, Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin-embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (bsm-33400M) at 1:400 overnight at 4\u00b0C, followed by a secondary antibody, Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bsm-33400M

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated(bsm-33401M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated(bsm-33401M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated(bsm-33401M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated(bsm-33401M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024)and DAB (C-0010) staining.

Catalog #1: bsm-33401m-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33400M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33400M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program. MCF-7 cells probed with Rabbit Anti-Collagen I\uffa0Polyclonal antibody (bs-10423R)at 1:1000.
This image was kindly submitted by Kandy Velazquez of USC School of Medicine.\r\nMouse adipose tissue lysates probed with Collagen I Polyclonal Antibody, unconjugated (bs-10423R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at room temperature.
Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Collagen I Antibody, Alexa Fluor 647 Conjugated (bs-10423R-AF647) at 1:400, for 1.5 hours at 37\u00b0C.
Lane 1: Mouse Ovary; Lane 2: NIH\/3T3; Lane 3: Rat Liver; Lane 4: 293T; Lane 5: A431; Lane 6: RAW264.7; Probed with Collagen I Polyclonal Antibody (bs-10423R) at 1:300 dilution overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n

Catalog #1: bs-10423r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10423r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-10423r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human esophageal carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with COL1A1\/Collagen I (5F2) Monoclonal Antibody, Unconjugated (bsm-52478R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Collagen I) Monoclonal Antibody, Unconjugated (bsm-52478R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Monoclonal Antibody, Unconjugated (bsm-52478R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Collagen I Monoclonal Antibody, Unconjugated (bsm-52478R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52478r-TR

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52478r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-52478r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Collagen II(9H12)) Monoclonal Antibody, Unconjugated (bsm-33409M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat fetal bone); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Collagen II(9H12)) Monoclonal Antibody, Unconjugated (bsm-33409M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Collagen II(9H12)) Monoclonal Antibody, Unconjugated (bsm-33409M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Collagen II(9H12)) Monoclonal Antibody, Unconjugated (bsm-33409M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33409m-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33382m-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-33409m-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33409m-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Lane 1: U2os lysates; Lane 2: MDA-MB-231 lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-11929R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: 293T cell lysates probed with Collagen II Polyclonal Antibody, Unconjugated (bs-11929R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n

Catalog #1: bs-11929R

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293T lysates; Lane 2: A549 lysates; Lane 3: LOVO lysates; Lane 4: MCF-7 lysates;Lane 5: HepG2 lysates probed with COL2A1 Polyclonal Antibody, Unconjugated (bs-10589R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Embryo lysates; Lane 2: MG63 lysates probed with COL2A1 Polyclonal Antibody, Unconjugated (bs-10589R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-10589R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-11929R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin