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Catalog #1: bs-23929R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-23929R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2607R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0529r

Applications: IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0529R-Biotin

Applications: IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0529R-HRP

Applications: IHC-P

Reactivity: Human

Conjugate: HRP

Lane 1: Normal HeLa cell lysates; Lane 2: HeLa cell treated with Brefeldin A (5 \u03bc g\/ml, 15 min) lysates;Lane 3: Jurkat cell lysates (Negative control ) probed with IL18 Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IL18) Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:250 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IL18) Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:250 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-IL18 antibody (bsm-56205R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-56205r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4040R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4040R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-4040R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0445R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11855R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11855R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-11855R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-1959R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1959R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-1959R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-1834R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-1834R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-1834R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-2621R

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2621R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-2621R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Formalin-fixed and paraffin embedded mouse lymphoma labeled with Anti-IL-21R Polyclonal Antibody, Unconjugated (bs-2622R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Thymus lysates probed with IL-21R Polyclonal Antibody, Unconjugated (bs-2622R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2622R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2622R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-2622R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Mouse splenocytes probed with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse spleen lysates probed with IL22BP Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Rat testis lysates; probed with IL-22BP\/IL22RA2 Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-2625R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse liver lysates Lane 2: mouse intestine probed with Rabbit Anti-IL-22R Polyclonal Antibody, Unconjugated (bs-2624R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse placenta lysates probed with IL-22R Polyclonal Antibody, Unconjugated (bs-2624R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2624R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2624R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-2624R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-10576R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10576R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-10576R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Antigen: bs-2711P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded mouse lymphoma labeled with Rabbit Anti IL-27R\/TCCR Polyclonal Antibody, Unconjugated (bs-2711R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with Rabbit Anti-IL-27RA Polyclonal Antibody, Unconjugated (bs-2711R) (green) at 1:100 for 40 minutes followed by a FITC conjugated secondary compared to control cells (blue).

Catalog #1: bs-2711R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2711R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-2711R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-IL2RA Polyclonal Antibody, Unconjugated (bs-0577R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: EC9706 lysates; Lane 2: mouse lymph node lysates probed with IL2RA Polyclonal Antibody, Unconjugated (bs-0577R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0577R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0577R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0577R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-2631R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2632R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Raji lysates probed with IL33 Polyclonal Antibody, Unconjugated (bs-2208R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (IL33) Polyclonal Antibody, Unconjugated (bs-2208R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (IL33) Polyclonal Antibody, Unconjugated (bs-2208R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (IL33) Polyclonal Antibody, Unconjugated (bs-2208R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2208R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Mouse splenocytes probed with Rabbit Anti-IL-33 Polyclonal Antibody, PE-Cy5 Conjugated (bs-2633R-PE-Cy5) (green) at 1:100 for 40 minutes compared to control cells (blue).
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Anti \u2013IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2633R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2633R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2633R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-62567r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62567r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-62567r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP