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Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-MEK5\/MAP2K5 Polyclonal Antibody, Unconjugated (bs-4124R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image provided by the Independent Validation Program, badge number 029644:Formalin-fixed and paraffin embedded human breast myoepithelial and glandular tissue labeled with Anti-MEK5 Polyclonal Antibody, Unconjugated (bs-4124R) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with Rabbit Anti-MEK5 Antibody, Unconjugated (bs-4124R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse heart lysates probed with MEK5 Polyclonal Antibody, Unconjugated (bs-4124R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4124R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4124R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-4124R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2911R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2911R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-2911R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-1979R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1979R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-1979R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MEK7) monoclonal Antibody, unconjugated (bsm-61322R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MEK7 antibody (bsm-61322R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with MEK7 monoclonal antibody, unconjugated (bsm-61322R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK7 Monoclonal Antibody, Unconjugated (bsm-61322R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61322r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61322r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-61322r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Flow cytometric analysis of HepG2 cells with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: HepG2; Lane 2: Rat brain; Lane 3: SW480 Cells; Probed with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52540R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52709r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52709r-biotin-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-52709r-hrp-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-2182M

Applications: ELISA, IHC-P, IF(IHC-P)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-2182M-Biotin

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: Biotin

Catalog #1: bsm-2182M-HRP

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MLANA\/MART1 Polyclonal Antibody (bs-0051R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) according to instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MLANA\/MART1 Polyclonal Antibody, Unconjugated (bs-0051R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0051R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7362r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0051R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0051R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Melan A Monoclonal Antibody, Unconjugated(bsm-52337R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with MART1 monoclonal antibody, unconjugated (bsm-52337R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-52337r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52337r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-52337r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-12201R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12201R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-12201R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-11322R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11322R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-11322R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Antigen: bs-1988P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Menin\/Men1 Polyclonal Antibody, Unconjugated (bs-1988R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti Menin\/Men1 Polyclonal Antibody, Unconjugated (bs-1988R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1988R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-1988R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-1988R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bsm-61541r-TR

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61541r-biotin-100ul

Applications: WB, IP

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-61541r-hrp-100ul

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-6056R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6056R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-6056R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MEPCE) polyclonal Antibody, unconjugated (bs-18788R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-MEPCE antibody (bs-18788R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bs-18788r-100ul

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-18788r-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-18788r-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Mouse uterus lysates probed with MEPE Polyclonal Antibody, Unconjugated (bs-8689R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Spleen tissue lysates; Lane 4: Mouse Cerebellum tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human MCF-7 cell lysates probed with MEPE Polyclonal Antibody, Unconjugated (bs-8689R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8689R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-8689R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-8689R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-0548R

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: Unconjugated