Free Shipping On Orders Over $1,000!

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Products

Collection Menu
Lane 1: K562 Cells; Lane 2: A431 Cells; Probed with CHAF1A (1D4) Monoclonal Antibody (bsm-54414R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CHAF1A (1D4) Monoclonal Antibody (bsm-54414R) 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CHAF1A (1D4) Monoclonal Antibody (bsm-54414R) 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CHAF1A (1D4) Monoclonal Antibody (bsm-54414R) 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54414R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1: SiHa Cells; Lane 2: K562 Cells; Lane 3: A431 Cells; Probed with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54416R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-6647R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-6647R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-70431M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CSNK1E Polyclonal Antibody, Unconjugated (bs-55048R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CSNK1E Polyclonal Antibody, Unconjugated (bs-55048R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: CSNK1E knockout (KO) HeLa cell lysates probed with CSNK1E Polyclonal Antibody, Unconjugated (bs-55048R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: 293T cell lysates probed with CSNK1E Polyclonal Antibody, Unconjugated (bs-55048R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55048R

Applications: WB, IHC-P, IF(ICC), IP, IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: PC-3M Cells; Probed with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54424R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1 : MCF7 Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : C6 Cell Lysate; Lane 4 : L929 Cell Lysate; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 4.0ug\/mL (1:250) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.
Lane 1 : Cyclin B1 Recombinant Protein; Lane 2 : Cyclin D1 Recombinant Protein; Lane 3 : Cyclin D2 Recombinant Protein; Lane 4 : Cyclin D3 Recombinant Protein; Lane 5 : Cyclin E1 Recombinant Protein; Lane 6 : Cyclin E2 Recombinant Protein; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-50375M

Applications: WB, IP

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-70443M

Applications: WB, IP, ICC

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 8 Monoclonal Antibody, Unconjugated (bsm-54512R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 8 Monoclonal Antibody, Unconjugated (bsm-54512R) at 1:50 for 30 minutes at room temperature, DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-54512R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (\r\nCytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-54512R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Catalog #1: bsm-54512R

Applications: WB, FCM, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-54606R

Applications: WB, IHC-P, IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55053R

Applications: WB, IP, IHC, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293T cell lysates; Lane 2: SW480 cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: DNMT1 knockout (KO) 293T cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
293T cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DNMT1) polyclonal Antibody, Unconjugated (bs-55056R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human Jurkat cell lysates; Lane 2: Human Molt-4 cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human 293T cell lysates ; Lane 5: Human U251 cell lysates ; Lane 6: Human HL60 cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55056R

Applications: WB, IF(ICC), IP, IF

Reactivity: Human

Conjugate: Unconjugated

Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Dnmt1(Ser154) Polyclonal Antibody(bs-5294R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Dnmt1(Ser154) Polyclonal Antibody, Unconjugated (bs-5294R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Dnmt1(Ser154) Polyclonal Antibody, Unconjugated (bs-5294R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Dnmt1(Ser154) Polyclonal Antibody, Unconjugated (bs-5294R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5294R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

To determine the titer of the antibody, an ELISA was performed using a serial dilution of Bioss antibody directed against human DNMT3A (Cat. No. bs-53128R), crude serum and Flow Through, in antigen-coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:500.
Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 \u03bcg of DNMT3A Polyclonal Antibody (Cat. No. bs-53128R). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with the anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3).

Catalog #1: bs-53128R

Applications: WB, ELISA, IP, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51128R

Applications: WB, ELISA, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70360M

Applications: WB, IP, IHC, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-70477M

Applications: WB, IP, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70478M

Applications: WB, IP, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70479M

Applications: WB, IP, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70481M

Applications: IP, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-54539R

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70492R

Applications: WB, IP, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55075R

Applications: WB, IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human H460 cell lysates probed with Fatty Acid Synthase Polyclonal Antibody, Unconjugated (bs-55077R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: Fatty Acid Synthase knockout (KO) 293T cell lysates probed with Fatty Acid Synthase Polyclonal Antibody, Unconjugated (bs-55077R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Fatty Acid Synthase) polyclonal Antibody, Unconjugated (bs-55077R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Fatty Acid Synthase Polyclonal Antibody, Unconjugated (bs-55077R) at 1:100 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-55077R

Applications: WB, IHC-P, IF(ICC), IP, IHC, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70501M

Applications: WB, IP, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70502M

Applications: WB, IP, ICC

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Brain; Lane 2: Rat Brain; Probed with Flotillin 1 Polyclonal Antibody, Unconjugated (bs-7798R) at 1:500 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C.
Lane 1: Mouse Heart tissue lysates; Lane 2: Rat Heart tissue lysates probed with Flotillin 1 Polyclonal Antibody, Unconjugated (bs-7798R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7798R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FosB Monoclonal Antibody, Unconjugated (bsm-54574R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FosB Monoclonal Antibody, Unconjugated (bsm-54574R) at 1:50 for 30 minutes at room temperature, DAB staining.

Catalog #1: bsm-54574R

Applications: WB, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-70503M

Applications: WB, IP, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70504M

Applications: WB, IP, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12066R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP, ICC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-12066R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-70505M

Applications: WB, IP, IHC, ICC

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse liver lysates probed with Anti-GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Rat Cerebrum lysates probed with GLP-1R Polyclonal Antibody, Unconjugated (bs-1559R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1559R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1559R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Lane 1: LO2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: HL60 cell lysates; Lane 4: HT-1080 cell lysates probed with GNAI3 Polyclonal Antibody, Unconjugated (bs-55087R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: GNAI3 knockout (KO) HeLa cell lysates probed with GNAI3 Polyclonal Antibody, Unconjugated (bs-55087R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55087R

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1: SiHa Cells; Probed with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54392R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GPA33 Monoclonal Antibody, Unconjugated (bsm-54676R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GPA33 Monoclonal Antibody, Unconjugated (bsm-54676R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GPA33 Monoclonal Antibody, Unconjugated (bsm-54676R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GPA33 Monoclonal Antibody, Unconjugated (bsm-54676R) at 1:200 for 30 minutes at room temperature, DAB staining.

Catalog #1: bsm-54676R

Applications: WB, IHC-P, IP, IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70510M

Applications: WB, IP, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: LO2 cell lysates; Lane 2: Hela cell lysates; Lane 3: 22Rv1 cell lysates; Lane 4: U251-MG cell lysates probed with GSK3B Polyclonal Antibody, Unconjugated (bs-55090R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: GSK3B knockout (KO) HeLa cell lysates probed with GSK3B Polyclonal Antibody, Unconjugated (bs-55090R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GSK3B ) polyclonal Antibody, Unconjugated (bs-55090R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55090R

Applications: WB, IF(ICC), IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

ChIP assays were performed using HeLa cells treated with colcemid, the H3K27me3S28p antibody (bs-53053R) and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 10,000 cells. A titration of the antibody consisting of 1, 5 and 10 \u03bcl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide (cat. No. sp-091-050) for 1 hour at room temperature. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and RPL30 and for the inactive gene MYOD. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
To determine the titer, an ELISA was performed using a serial dilution of the H3K27me3S28p antibody (bs-53053R). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,300.
A Dot Blot analysis was performed to test the cross reactivity of the H3K27me3S28p antibody (bs-53053R) with peptides containing other modifications of histone H3 and H4 and unmodified sequences from histone H3. One hundred to 0.2 pmol of the peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the peptide containing the modifications of interest.
Hela cells were stained with the H3K27me3S28p antibody (bs-53053R) and with DAPI. Cells were fixed with formaldehyde, permeabilized with sodium citrate and Triton X100 and blocked with PBS containing 2.5% BSA. Left side: cells were immunofluorescently labelled with the H3K27me3S28p antibody (diluted 1:200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to DyLight 488. Right side: the nuclei were stained with DAPI, which specifically labels DNA. Phosphorylation of H3 on serine 28 is increased during late G2 phase and reaches a maximum in metaphase cells. This may explain the different staining intensities of different cells.

Catalog #1: bs-53053R

Applications: WB, ELISA, IP, IF, dot-blot, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51053R

Applications: WB, ELISA, IP, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51069R

Applications: WB, ELISA, IP, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

HeLa cells were stained with the Bioss antibody against H4K5,8,12ac (cat. No. bs-53096R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. Figure A: cells were immunofluorescently labeled with the H4K5,8,12ac antibody (left) diluted 1:500 in blocking solution followed by Alexa488 conjugated secondary antibody. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. Figure B, C, D, and E: staining of the cells with the H4K5,8,12ac antibody after incubation of the antibody with 10 ng\/\u03bcl of the following blocking peptides: H4K5,8,12 unmodified (figure B), H4K5,8,12ac (figure C), H2A.ZK5,7,11ac (figure D) and H4K5,8,12,16ac (figure E).
Western blot was performed on whole cell (25 \u03bcg, lane 1) and histone extracts (15 \u03bcg, lane 2) of HeLa cells, and on 1 \u03bcg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Bioss antibody against H4K5,8,12ac (cat. No. bs-53096R). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
To test the cross-reactivity of the Bioss antibody against H4K5,8,12ac (cat. No. bs-53096R), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody directed against H4K5,8,12ac (cat. No. bs-53096R) in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,500.

Catalog #1: bs-53096R

Applications: WB, ELISA, IP, IF, dot-blot, ChIP-seq

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2 : Bosc23 cell lysate transfected with Myc\/His\/HePTP; Lane 3 : Bosc23 cell lysate transfected with GFP\/Ub+1 lysates, probed with HA tag (7H5) Monoclonal Antibody, unconjugated (bsm-50131M) at 1:10000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of Bosc23 cell lysates using HA tag (7H5) \r\nMonoclonal Antibody (bsm-50131M): Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2: Precipitated from 200ug Bosc23 transfected with HA\/HSP60 using 5ug of antibody; Lane 3 : Precipitated from PBS using 5ug of antibody; WB analysis was performed using bsm-50131M at 0.1ug\/mL (1:10000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #1: bsm-50131M

Applications: WB, IF(ICC), IP

Reactivity: Human, All

Conjugate: Unconjugated

Lane 1: 293T cell lysates; Lane 2: HADHA knockout (KO) 293T cell lysates probed with HADHA Polyclonal Antibody, Unconjugated (bs-55092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: THP-1 cell lysates; Lane 3: SW480 cell lysates; Lane 4: PC-3 cell lysates; Lane 5: Hela cell lysates; Lane 6: Mouse Intestine lysates; Lane 7: Mouse Heart lysates; Lane 8: Mouse Fat lysates probed with HADHA Polyclonal Antibody, Unconjugated (bs-55092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HADHA) polyclonal Antibody, Unconjugated (bs-55092R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HADHA) polyclonal Antibody, Unconjugated (bs-55092R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55092R

Applications: WB, IF(ICC), IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human 293T cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Mouse Thymus lysates; Lane 6: Mouse Testis lysates; Lane 7: Rat Thymus lysates; Lane 8: Rat Testis lysates probed with HDAC1 Polyclonal Antibody, Unconjugated (bs-55093R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: HDAC1 knockout (KO) 293T cell lysates probed with HDAC1 Polyclonal Antibody, Unconjugated (bs-55093R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HDAC1) polyclonal Antibody, Unconjugated (bs-55093R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HDAC1 Polyclonal Antibody, Unconjugated (bs-55093R) at 1:100 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-55093R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IP, IF

Reactivity: Human

Conjugate: Unconjugated