Receive 30% off all Monoclonal IHC antibodies with code "MONO-IHC-30"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Products



×

Collection Menu

Catalog #1: bsm-42106m-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-42106m-rbitc-100ul

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-51481m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51124M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51180M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Estrogen receptor alpha) Monoclonal Antibody, Unconjugated (bsm-60803R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human endometrium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Estrogen receptor alpha) Monoclonal Antibody, Unconjugated (bsm-60803R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with Estrogen receptor alpha monoclonal antibody, unconjugated (bsm-60803R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Estrogen receptor alpha antibody (bsm-60803R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-60803r

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-54724R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60803r-bf350-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-60803r-bf405-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-60803r-bf488-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-60803r-bf555-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-60803r-bf594-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-60803r-bf647-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-60803r-bf680-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-60803r-bf750-100ul

Applications: IF

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-60803r-apc-100ul

Applications: IF

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-60803r-apc-Cy5.5-100ul

Applications: IF

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-60803r-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-60803r-cy3-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-60803r-cy5-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-60803r-Cy5.5-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-60803r-cy7-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-60803r-fitc-100ul

Applications: IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-60803r-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-60803r-pe-100ul

Applications: IF

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-60803r-percp-100ul

Applications: IF

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-60803r-percp-Cy5.5-100ul

Applications: IF

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-60803r-rbitc-100ul

Applications: IF

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-61360r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61360r-bf350-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61360r-bf405-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61360r-bf488-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61360r-bf555-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61360r-bf594-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61360r-bf647-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61360r-bf680-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61360r-bf750-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61360r-apc-100ul

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-61360r-apc-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-61360r-apc-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-61360r-biotin-100ul

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-61360r-cy3-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-61360r-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-61360r-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-61360r-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-61360r-fitc-100ul

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-61360r-hrp-100ul

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-61360r-pe-100ul

Applications: WB

Reactivity: Human

Conjugate: PE