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Catalog #1: bsm-62578r-cy3-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-62578r-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-62578r-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-62578r-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-62578r-fitc-100ul

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-62578r-hrp-100ul

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-62578r-pe-100ul

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-62578r-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-62578r-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-62578r-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-62578r-percp-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-62578r-percp-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-62578r-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-62578r-rbitc-100ul

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-33182M

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33182M-BF350

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33182M-BF405

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33182M-BF488

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33182M-BF555

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33182M-BF594

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33182M-BF647

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33182M-BF680

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33182M-BF750

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33182M-APC

Applications: WB

Reactivity: Species independent

Conjugate: APC

Catalog #1: bsm-33182M-APC-Cy5.5

Applications: WB

Reactivity: Species independent

Conjugate: APC-Cy5.5

Catalog #1: bsm-33182M-APC-Cy7

Applications: WB

Reactivity: Species independent

Conjugate: APC-Cy7

Catalog #1: bsm-33182M-Biotin

Applications: WB

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-33182M-Cy3

Applications: WB

Reactivity: Species independent

Conjugate: Cy3

Catalog #1: bsm-33182M-Cy5

Applications: WB

Reactivity: Species independent

Conjugate: Cy5

Catalog #1: bsm-33182M-Cy5.5

Applications: WB

Reactivity: Species independent

Conjugate: Cy5.5

Catalog #1: bsm-33182M-Cy7

Applications: WB

Reactivity: Species independent

Conjugate: Cy7

Catalog #1: bsm-33182M-FITC

Applications: WB

Reactivity: Species independent

Conjugate: FITC

Catalog #1: bsm-33182M-HRP

Applications: WB

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-33182M-PE

Applications: WB

Reactivity: Species independent

Conjugate: PE

Catalog #1: bsm-33182M-PE-Cy5

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy5

Catalog #1: bsm-33182M-PE-Cy5.5

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy5.5

Catalog #1: bsm-33182M-PE-Cy7

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy7

Catalog #1: bsm-33182M-PerCP

Applications: WB

Reactivity: Species independent

Conjugate: PerCP

Catalog #1: bsm-33182M-PerCP-Cy5.5

Applications: WB

Reactivity: Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33182M-RBITC

Applications: WB

Reactivity: Species independent

Conjugate: RBITC

Catalog #1: bsm-53018M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: 293T Cells; Lane 2: 293 Cells; Probed with EZH2 (9B6) Monoclonal Antibody (bsm-52743R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with EZH2 (9B6) Monoclonal Antibody (bsm-52743R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with EZH2 (9B6) Monoclonal Antibody (bsm-52743R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with EZH2 (9B6) Monoclonal Antibody (bsm-52743R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52743R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (EZH2) Monoclonal Antibody, Unconjugated (bsm-51468M) at 1:50 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Jurkat cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with EZH2 Antibody(bsm-51468M)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with EZH2 monoclonal antibody, unconjugated (bsm-51468M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51468m

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Cell line: HeLa\t<br>\r\nFixative: 4% Paraformaldehyde\t<br>\r\nPermeabilization: 0.1% TritonX-100\t<br>\r\nPrimary ab dilution: 1:50\t<br>\r\nPrimary incubation condition: 4\u00b0C overnight\t<br>\r\nSecondary ab: Goat Anti-Rabbit IgG\t<br>\r\nNuclear counter stain: DAPI (Blue)\t<br>\r\nComment: Color green is the positive signal for bsm-60001R <br>
25 ug total protein per lane of various lysates (see on figure) probed with EZH2 monoclonal antibody, unconjugated (bsm-60001R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EZH2 Monoclonal Antibody, Unconjugated(bsm-60001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EZH2 Monoclonal Antibody, Unconjugated(bsm-60001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60001r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-63051r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-63051r-bf350-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-63051r-bf405-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-63051r-bf488-100ul

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488