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Catalog #1: bs-93527c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-99752c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-93488c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-99086c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-90130c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-90131c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-94309c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-89944c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-91214c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-99647c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-99649c-2mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-99993c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-90679c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-85639c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-85640c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-85641c-2mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-87007c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2765R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-87384c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-90682c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-96585c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-90287c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-92024c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-91221c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-91222c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-87385c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BNC1 Polyclonal Antibody, Unconjugated (bs-22170R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BNC1 Polyclonal Antibody, Unconjugated (bs-22170R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BNC1 Polyclonal Antibody, Unconjugated (bs-22170R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-22170R

Applications: IHC-P, IHC-F, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-20583r

Applications: IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6681R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with BNIP1 monoclonal antibody, unconjugated (bsm-61513R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The A431 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-BNIP1 antibody (bsm-61513R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-61513r-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6682R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-4239R

Applications: WB, ELISA, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BNIP3 Monoclonal Antibody, Unconjugated(bsm-61440R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61440r-TR

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-5798R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-61515r-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-89840c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-25434pk-1mg

Applications: Others

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-25435pk-1mg

Applications: Others

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BNP Polyclonal Antibody, Unconjugated (bs-2207R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-BNP Polyclonal Antibody, Unconjugated(bs-2207R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-2207R

Applications: ELISA, IHC-P, IF(IHC-P)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-61539r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-93835c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82989c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nLysate: Ramos, Daudi, Raji\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 27 kDa\r\nObserved MW: 35 kDa\r\n
Tissue: Human lymphoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60269R

Catalog #1: bsm-60269r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated (bsm-54659R) at 1:200 for 30 minutes at room temperature, DAB staining.
Flow cytometric analysis of BOB1 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54659R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Catalog #1: bsm-54659R

Applications: WB, FCM, IHC-P, IHC

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded \tHuman Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded \tHuman Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

Catalog #1: bsm-34182m-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12876R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-1418R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-95593c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated