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Catalog #1: bs-53083R

Applications: ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-90089r-50uL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62125r

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Hela cells with CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54154R

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-53080R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nCBX3 Polyclonal Antibody, Unconjugated (bs-55037R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nCBX3 Polyclonal Antibody, Unconjugated (bs-55037R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: 293T cell lysates; Lane 4: A549 cell lysates; Lane 5: NCI-H460 cell lysates; Lane 6: Mouse Brain lysates; Lane 7: Mouse Lung lysates; Lane 8: Mouse Spleen lysates probed with CBX3 Polyclonal Antibody, Unconjugated (bs-55037R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: CBX3 knockout (KO) HeLa cell lysates probed with CBX3 Polyclonal Antibody, Unconjugated (bs-55037R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55037R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-51080R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse testis lysates; Lane 2: Human HepG2 lysates probed with CBX4 Polyclonal Antibody, Unconjugated (bs-17376R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Liver tissue lysates; Lane 3: Human K562 cell lysates; Lane 4: Human HL-60 cell lysates; Lane 5: Human HeLa cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human HUVEC cell lysates; Lane 8: Human A549 cell lysates probed with CBX4 Polyclonal Antibody, Unconjugated (bs-17376R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CBX4) polyclonal Antibody, Unconjugated (bs-17376R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-17376R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-53090R

Applications: ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51090R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: HepG2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 61 kDa\r\nObserved MW: 61 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: C6\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 61 kDa\r\nObserved MW: 61 kDa
Tissue: Human breast carcinoma\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60681R
Tissue: Human cerebrum\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60681R

Catalog #1: bsm-60681r

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Hela; Probed with CBX5 (3G1) Monoclonal Antibody (bsm-52301R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CBX5 (3G1) Monoclonal Antibody (bsm-52301R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CBX5 (3G1) Monoclonal Antibody (bsm-52301R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CBX5 (3G1) Monoclonal Antibody (bsm-52301R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52301R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61683r-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51407M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51593m

Applications: WB, Flow-Cyt

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-20164R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-87443c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-88581c-100mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-88582c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-88583c-2mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-88584c-100mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-88585c-50mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-88586c-50mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6483c-10mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-87445c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-100073c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-87446c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82729c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6544c-10mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-93433c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-CC2D1A antibody (bsm-62421R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
25 ug total protein per lane of various lysates (see on figure) probed with CC2D1A monoclonal antibody, unconjugated (bsm-62421R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CC2D1A) monoclonal Antibody, unconjugated (bsm-62421R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-62421r-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-100126c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-9405R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9859R

Applications: ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Other Species

Conjugate: Unconjugated

Catalog #1: bs-85703c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7985R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-10939R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

U87MG cell lysates probed with CCBR1 Polyclonal Antibody, Unconjugated (bs-6883R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human HeLa cell lysates probed with CCBR1 Polyclonal Antibody, Unconjugated (bs-6883R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6883r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61613r-100ul

Applications: WB, IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-90930c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-97588c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-98916c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60310M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-8103R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CCDC106 Polyclonal Antibody, Unconjugated (bs-5711R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CCDC106 Polyclonal Antibody, Unconjugated (bs-5711R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5711R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CCDC11 Polyclonal Antibody, Unconjugated (bs-7740R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti CCDC11 Polyclonal Antibody, Unconjugated (bs-7740R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 57kD. Observed band size: 57kD
Antigen: bs-7740P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
A431 cell lysates probed with CCDC11 Polyclonal Antibody, Unconjugated (bs-7740R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7740R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8084R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-8095R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated