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Catalog #1: bs-0428R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-70452r

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70451r

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62128r

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10400R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-100695c-5g

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-97978c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82845c-50mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11588R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11549R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-92149c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-95772c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-95503c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-89102c-100mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-95155c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-99076c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-89188c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-96192c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-100273c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82794c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-95860c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-92334c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-87567c-1ml10mmin-h2o

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82694c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82713c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-98164c-2mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-90146c-10mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6956c-10mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-87568c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-13007R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with DKC1 monoclonal antibody, unconjugated (bsm-61870R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-DKC1 antibody (bsm-61870R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DKC1 Monoclonal Antibody, Unconjugated (bsm-61870R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat\r\n Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DKC1 Monoclonal Antibody, Unconjugated (bsm-61870R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61870r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-97580c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-DKK1 Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded canine mammary tumor labeled with Rabbit Anti-DKK1 Polyclonal Antibody, Unconjugated (bs-2162R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-2162R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Dog

Conjugate: Unconjugated

Catalog #1: bs-100639c-50ug

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-100503c-20ug

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with DKK1 monoclonal antibody, unconjugated (bsm-61289R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DKK1 Monoclonal Antibody, Unconjugated(bsm-61289R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DKK1 Monoclonal Antibody, Unconjugated(bsm-61289R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DKK1 Monoclonal Antibody, Unconjugated(bsm-61289R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61289r-TR

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51690m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2686R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-97690c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-94759c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-101855c-1g

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-99948c-200mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-76397c-1g

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-76402c-250mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-94155c-100mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-94486c-25mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-85709c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-85708c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated