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Catalog #1: bs-85929c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-94723c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-85930c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-96016c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-85932c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-98570c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-85933c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-90019c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded EGFR5 Polyclonal Antibody; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with EGFR5 Polyclonal Antibody, Unconjugated (bs-9813R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Human 293T cells probed with EGFR5 Polyclonal Antibody, Unconjugated (bs-9813R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).\\n
Lane 1: B16 cell lysates; Lane 2: DU145 cell lysates probed with EGFR5 Polyclonal Antibody, Unconjugated (bs-9813R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9813R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-EGFRvIII Polyclonal Antibody, Unconjugated (bs-2558R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
A549 Cells lysates probed with EGFRvIII Polyclonal Antibody, unconjugated (bs-2558R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (EGFRvIII) polyclonal Antibody, Unconjugated (bs-2558R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2558r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-101700c-10mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGLN1 Polyclonal Antibody, Unconjugated (bs-55063R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGLN1 Polyclonal Antibody, Unconjugated (bs-55063R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGLN1 Polyclonal Antibody, Unconjugated (bs-55063R) at 1:100 overnight at 4\u00b0C, DAB staining.
L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (EGLN1) polyclonal Antibody, Unconjugated (bs-55063R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55063R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-85934c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat small intestine labeled with Anti-Egr1 Polyclonal Antibody, Unconjugated (bs-1076R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse Lung lysates probed with Egr1 Polyclonal Antibody, Unconjugated (bs-1076R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.01%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Egr1 Polyclonal Antibody(bs-1076R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Egr1) polyclonal Antibody, Unconjugated (bs-1076R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1076R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EGR1 Monoclonal Antibody, Unconjugated(bsm-61687R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EGR1 Monoclonal Antibody, Unconjugated(bsm-61687R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61687r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates probed with EGR2 Polyclonal Antibody, Unconjugated (bs-8368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGR2 Polyclonal Antibody, Unconjugated (bs-8368R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-8368R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-61927r-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6448R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with EHD1 monoclonal antibody, unconjugated (bsm-62011R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-EHD1 antibody (bsm-62011R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EHD1 Monoclonal Antibody, Unconjugated (bsm-62011R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EHD1 Monoclonal Antibody, Unconjugated (bsm-62011R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62011r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4058R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

To determine the titer, an ELISA was performed using a serial dilution of the EHMT1 antibody (bs-53068R). The wells were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,000.
Nuclear extracts of HeLa cells (40 \u03bcg) were analyzed by Western blot using the EHMT1 antibody (bs-53068R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment may represent a splicing variant.

Catalog #1: bs-53068R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51068r-100ul

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60315m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90322r-50uL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EHMT2 Monoclonal Antibody, Unconjugated (bsm-61806R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EHMT2 Monoclonal Antibody, Unconjugated (bsm-61806R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EHMT2 Monoclonal Antibody, Unconjugated (bsm-61806R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EHMT2 Monoclonal Antibody, Unconjugated (bsm-61806R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61806r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-82492c-10mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-86881c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-87598c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6449c-10mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-96204c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-96205c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3839R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-97906c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-101555c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (EID1) Polyclonal Antibody, Unconjugated (bs-6138R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (EID1) Polyclonal Antibody, Unconjugated (bs-6138R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-6138R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75614c-10mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-EIF2S1 (Ser51) Monoclonal Antibody, Unconjugated(bsm-52306R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-EIF2S1 (Ser51) Monoclonal Antibody, Unconjugated(bsm-52306R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-EIF2S1 (Ser51) Monoclonal Antibody, Unconjugated(bsm-52306R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-EIF2S1 (Ser51) Monoclonal Antibody, Unconjugated(bsm-52306R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52306r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13066R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with eIF1AX monoclonal antibody, unconjugated (bsm-61809R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-eIF1AX antibody (bsm-61809R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF1AX Monoclonal Antibody, Unconjugated (bsm-61809R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF1AX Monoclonal Antibody, Unconjugated (bsm-61809R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61809r-TR

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with eIF2A monoclonal antibody, unconjugated (bsm-61990R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The U-87MG (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-eIF2A antibody (bsm-61990R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-61990r-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: HepG2 Cell lysates; Lane 3: SH-SY5Y Cell lysates; probed with EIF2AK2 (1C4) Monoclonal Antibody, unconjugated (bsm-51436M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with EIF2AK2 (1C4) Monoclonal Antibody (bsm-51436M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).

Catalog #1: bsm-51436M

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti EIF2AK2\/PKR Polyclonal Antibody, Unconjugated (bs-1493R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-EIF2AK2\/PKR Polyclonal Antibody, Unconjugated (bs-1493R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-EIF2AK2\/PKR Polyclonal Antibody, Unconjugated (bs-1493R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Hela cell lysates; Lane 2: 293T cell lysates probed with EIF2AK2\/PKR Polyclonal Antibody, Unconjugated (bs-1493R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1493R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51601m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH\/3T3 cell lysates probed with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-51733M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH\/3T3 cell lysates; Lane 5: HepG2 cell lysates probed with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-51733M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51733m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Eif2ak2 Polyclonal Antibody, Unconjugated (bs-55064R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: Jurkat cell lysates probed with Eif2ak2 Polyclonal Antibody, Unconjugated (bs-55064R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Eif2ak2 ) polyclonal Antibody, Unconjugated (bs-55064R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55064R

Applications: WB, IHC-P, IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63145r-100ul

Applications: WB, IHC-P, IHC-F, IP, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51385m

Applications: WB, IHC-P

Reactivity: Mouse, Rat

Conjugate: Unconjugated

IF(ICC) staining with EIF2AK4 (6G6) Monoclonal Antibody (bsm-54039R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with EIF2AK4 (6G6) Monoclonal Antibody (bsm-54039R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with EIF2AK4 (6G6) Monoclonal Antibody (bsm-54039R) at 1:100 in MecF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with EIF2AK4 (6G6) Monoclonal Antibody (bsm-54039R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54039R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated