RECEIVE 25% OFF PUBLISHED ANTIBODIES WITH CODE "PUB25"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Products



×

Collection Menu
Western blot was performed on whole cell (25\u03bcg, lane 1) and histone extracts (15\u03bcg, lane 2) from HeLa cells, and on 1 \u03bcg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H4K5ac Polyclonal Antibody (bs-53099R). Antibody was diluted 1:500 in TBS-Tween containing 5% skim milk.
Dot Blot analysis was performed with peptides containing a variety of histone modifications and the unmodified H4. 0.2-100 pmol of the respective peptides were spotted on a membrane. H4K5ac Polyclonal Antibody (bs-53099R) was used at 1:5,000 dilution. Result shows high specificity of the antibody for the modification of interest.
ChIP were performed using HeLa cells using H4K5ac Polyclonal Antibody (bs-53099R). Chromatin was sheared from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10\u03bcg per ChIP experiment was analyzed. IgG (2\u03bcg\/IP) was used as negative IP control. Quantitative PCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH as positive controls, and for the inactive MYOD1 gene and Sat2 satellite repeat region used as negative controls. Result shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ELISA was performed using a serial dilution of the\r\nH4K5ac Polyclonal Antibody (bs-53099R) against peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,900.

Catalog #1: bs-53099R

Applications: WB, ELISA, IHC-P, IF(ICC), dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51099r-50ug

Applications: WB, ELISA, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H4K8ac Polyclonal Antibody (bs-53063R). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:17,500.
A Dot Blot analysis was performed to test the cross-reactivity of \r\nH4K8ac Polyclonal Antibody (bs-53063R) with peptides containing other modifications of histone H4 and H3 and an unmodified histone H4 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest.
Histone extracts of HeLa cells (15 \u03bcg) were analyzed by Western blot using the antibody against H4K8ac (bs-53063R) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right. Lane 2 shows the result of the Western analysis with the antibody; lane 1 shows the same analysis after incubation of the antibody with 750 pmol blocking peptide for 1 hour at room temperature.
ChIP assays were performed using HeLa cells treated with colcemid, H4K8ac Polyclonal Antibody (bs-53063R) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 10,000 cells. A titration of the antibody consisting of 0.5, 1.5, and 5 \u03bcl per ChIP experiment was analyzed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes ALDOA and MYOD. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bs-53063R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

A Dot Blot analysis was performed to test the cross-reactivity of the Bioss antibody against H4K8ac (cat. No. bs-53133R) with peptides containing other histone modifications and the unmodified H4K8. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000.
Histone extracts of HeLa cells (15 \u03bcg) were analyzed by Western blot using the Bioss antibody directed against H4K8ac (cat. No. bs-53133R) diluted 1:200 in TBS-Tween containing 5% skimmed milk.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody directed against H4K8ac (cat. No. bs-53133R), crude serum and flow through in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:16,700.
Mouse NIH3T3 cells were stained with H4K8ac Polyclonal Antibody (cat. No. bs-53133R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K8ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Catalog #1: bs-53133R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-51063r-100ul

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51133r-50ug

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

Western blot was performed on whole cell extracts (25 \u03bcg, lane 1) and histone extracts (15 \u03bcg, lane 2) from HeLa cells, and on 1 \u03bcg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H4pan Polyclonal Antibody(bs-53138R). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H4pan Polyclonal Antibody (bs-53138R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the H4 antibody (middle) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of H4pan Polyclonal Antibody (bs-53138R) in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,000.
ChIP assays were performed using HeLa cells, H4pan Polyclonal Antibody (bs-53138R) and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells on the IP-Star Compact automated system. A titration consisting of 0.5, 1, 2 and 5 \u03bcg of antibody per ChIP experiment was analyzed. IgG (1 \u03bcg\/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and c-fos genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bs-53138R

Applications: WB, ELISA, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51138r-50ug

Applications: ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2284R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-2285R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-0344R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-4813R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bsm-49058M

Applications: WB

Reactivity: H5N6

Conjugate: Unconjugated

Catalog #1: bs-10298R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-82411c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-90061c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-102317c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-0966M

Applications: WB, IF(ICC)

Reactivity: Tags

Conjugate: Unconjugated

Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2 : Bosc23 cell lysate transfected with Myc\/His\/HePTP; Lane 3 : Bosc23 cell lysate transfected with GFP\/Ub+1 lysates, probed with HA tag (7H5) Monoclonal Antibody, unconjugated (bsm-50131M) at 1:10000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of Bosc23 cell lysates using HA tag (7H5) \r\nMonoclonal Antibody (bsm-50131M): Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2: Precipitated from 200ug Bosc23 transfected with HA\/HSP60 using 5ug of antibody; Lane 3 : Precipitated from PBS using 5ug of antibody; WB analysis was performed using bsm-50131M at 0.1ug\/mL (1:10000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #1: bsm-50131M

Applications: WB, IF(ICC), IP

Reactivity: Human, All

Conjugate: Unconjugated

Catalog #1: bsm-33003m

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bsm-33157m

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bs-51024R

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33157r-100ul

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Lane 1: E.coli Lysate lysates probed with HA tag Polyclonal Antibody, Unconjugated (bs-0966R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4 ug\r\nPrimary: Anti-HA tag (bs-0966R) at 1\/1000 ~ 1\/10000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution

Catalog #1: bs-0966R

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bs-82343c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-91378c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-52887R

Applications: WB

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bs-96917c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-98067c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11695R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Mouse kidney lysates probed with HABP4 Polyclonal Antibody, Unconjugated (bs-15402R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Muscle tissue lysates probed with HABP4 Polyclonal Antibody, Unconjugated (bs-15402R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-15402R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51691m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-6011R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-92224c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Lane 1: 293T cell lysates; Lane 2: HADHA knockout (KO) 293T cell lysates probed with HADHA Polyclonal Antibody, Unconjugated (bs-55092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: THP-1 cell lysates; Lane 3: SW480 cell lysates; Lane 4: PC-3 cell lysates; Lane 5: Hela cell lysates; Lane 6: Mouse Intestine lysates; Lane 7: Mouse Heart lysates; Lane 8: Mouse Fat lysates probed with HADHA Polyclonal Antibody, Unconjugated (bs-55092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HADHA) polyclonal Antibody, Unconjugated (bs-55092R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HADHA) polyclonal Antibody, Unconjugated (bs-55092R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55092R

Applications: WB, IF(ICC), IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Siha cell lysates probed with HADHB Polyclonal Antibody, Unconjugated (bs-5065R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: SY5Y cell lysates probed with HADHB Polyclonal Antibody, Unconjugated (bs-5065R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5065R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas labeled with Rabbit Anti-HADHSC Polyclonal Antibody, Unconjugated (bs-10020R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse liver lysates probed with Rabbit Anti-HADHSC Polyclonal Antibody (bs-10020R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.\\n
Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Pancreas tissue lysates probed with HADHSC Polyclonal Antibody, Unconjugated (bs-10020R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-10020R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3661R

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-102375c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-102368c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-102381c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-102371c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-15403R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bacteria

Conjugate: Unconjugated

Catalog #1: bsm-51205M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-15404R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-8414R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0540R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-Hairless Polyclonal Antibody, Unconjugated (bs-8507R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat spleen labeled with Anti-Hairless Polyclonal Antibody, Unconjugated (bs-8507R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Antigen: bs-8307P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm.
Lane 1: Siha cell lysates; Lane 2: A549 cell lysates probed with Hairless Polyclonal Antibody, Unconjugated (bs-8507R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8507R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated