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Catalog #1: bs-6387R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11889R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-54052r-100ul

Applications: WB, FCM, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-11885R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MEIS2 antibody (bsm-61073R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
25 ug total protein per lane of various lysates (see on figure) probed with MEIS2 monoclonal antibody, unconjugated (bsm-61073R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-61073r-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-88738c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-91489c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with phospho-MEK1 (Ser298) monoclonal antibody, unconjugated (bsm-52175R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-MEK1 (Ser298) Monoclonal Antibody, Unconjugated(bsm-52175R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-MEK1 (Ser298) Monoclonal Antibody, Unconjugated(bsm-52175R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-MEK1 (Ser298) Monoclonal Antibody, Unconjugated(bsm-52175R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52175r

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-70153r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-70154r

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-MEK1\/2(MAPKK1) Polyclonal Antibody, Unconjugated (bs-1041R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-MEK1\/2 Polyclonal Antibody, Unconjugated (bs-1041R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-1041R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Images provided the Independent Validation Program (badge number 029646)Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-MEK1 Polyclonal Antibody (bs-1433R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody. \\n
Image provided by the Independent Validation Program (badge number 29760). Lane 1: HeLa cell extract, Lane 2: NIH\/3T3 cell extracts, Lane 3: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-MEK1 Polyclonal Antibody, Unconjugated (bs-1433R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK1 Polyclonal Antibody, Unconjugated (bs-1433R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-1433R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MEK1 Monoclonal Antibody, Unconjugated(bsm-60744R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MEK1 Monoclonal Antibody, Unconjugated(bsm-60744R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MEK1 Monoclonal Antibody, Unconjugated(bsm-60744R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MEK1 Monoclonal Antibody, Unconjugated(bsm-60744R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60744r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70152r

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

HepG2 cell lysate probed with MEK2 (4C3) Monoclonal Antibody (bsm-33216M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK2 Monoclonal Antibody (bsm-33216M) at 1:400 overnight at 4\u00b0C, followed by SP Kit(Mouse) according to instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK2 Monoclonal Antibody (bsm-33216M) at 1:400 overnight at 4\u00b0C, followed by SP Kit(Mouse) according to instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK2 (4C3) Monoclonal Antibody, Unconjugated (bsm-33216M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33216M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MEK2 Monoclonal Antibody, Unconjugated (bsm-63042R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MEK2 Monoclonal Antibody, Unconjugated (bsm-63042R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MEK2 Monoclonal Antibody, Unconjugated (bsm-63042R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MEK2 Monoclonal Antibody, Unconjugated (bsm-63042R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-63042r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2\/MAPKK2 Polyclonal Antibody, Unconjugated (bs-0223R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Images provided by the Independent Validation Program, badge number 029748. Lane one: HeLa cell lysates. Lane 2: c6\/36 Mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-MEK2 Polyclonal Antibody, Unconjugated (bs-0223R) at 1:100 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250. Followed by Goat Anti-Rabbit antibody conjugated to Alexa fluor 488 at 1:500 dilution.

Catalog #1: bs-0223R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with MEK3 (4C1) Monoclonal Antibody (bsm-52658R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Hela cell lysate probed with MEK3 (4C1) Monoclonal Antibody (bsm-52658R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with MEK3 (4C1) Monoclonal Antibody (bsm-52658R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse spleen tissue incubated with MEK3 (4C1) Monoclonal Antibody (bsm-52658R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52658R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-MEK3\/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK3\/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: rat skeletal muscle lysates probed with MEK3\/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1689R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60662r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-63167r-100ul

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-70155r

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-MEK5\/MAP2K5 Polyclonal Antibody, Unconjugated (bs-4124R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image provided by the Independent Validation Program, badge number 029644:Formalin-fixed and paraffin embedded human breast myoepithelial and glandular tissue labeled with Anti-MEK5 Polyclonal Antibody, Unconjugated (bs-4124R) at 1:250 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with Rabbit Anti-MEK5 Antibody, Unconjugated (bs-4124R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse heart lysates probed with MEK5 Polyclonal Antibody, Unconjugated (bs-4124R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4124R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63027r

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2911R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-1979R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MEK7) monoclonal Antibody, unconjugated (bsm-61322R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MEK7 antibody (bsm-61322R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with MEK7 monoclonal antibody, unconjugated (bsm-61322R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK7 Monoclonal Antibody, Unconjugated (bsm-61322R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61322r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: HepG2; Lane 2: Rat brain; Lane 3: SW480 Cells; Probed with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52540R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52709r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-86270c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-71146r

Applications: ELISA/RIA

Reactivity: Animal

Conjugate: Unconjugated

Catalog #1: bs-94961c-5g

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-2182M

Applications: ELISA, IHC-P, IF(IHC-P)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-2182pg-1mg

Applications: Others

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0904pb-1mg

Applications: Others

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2182pb-1mg

Applications: Others

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0904pk-1mg

Applications: Others

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2182pk-1mg

Applications: Others

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2182pa-1mg

Applications: Others

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MLANA\/MART1 Polyclonal Antibody (bs-0051R) at 1:400 overnight at 4\u00b0C, followed by SP Kit (Rabbit) according to instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MLANA\/MART1 Polyclonal Antibody, Unconjugated (bs-0051R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0051R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7362r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Melan A Monoclonal Antibody, Unconjugated(bsm-52337R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with MART1 monoclonal antibody, unconjugated (bsm-52337R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-52337r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-102253c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-102672c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-102344c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-102250c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-76561c-5g

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-89730c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated