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Catalog #1: bsm-33413M-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: PE-Cy5.5

Catalog #1: bsm-33412M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: PE-Cy7

Catalog #1: bsm-33413M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: PE-Cy7

Catalog #1: bsm-33412M-PerCP

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: PerCP

Catalog #1: bsm-33413M-PerCP

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: PerCP

Catalog #1: bsm-33412M-PerCP-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33413M-PerCP-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33412M-RBITC

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: RBITC

Catalog #1: bsm-33413M-RBITC

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: RBITC

Formalin-fixed and paraffin embedded rat kidney tissue labeled withAnti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032r) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysates, L2 mouse brain lysates probed with Anti- Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. P

Catalog #1: bs-0032r

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0466R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Sheep, Goat, Cow

Conjugate: Unconjugated

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta-Actin (1A2) Monoclonal Antibody(bsm-33036M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin monoclonal antibody, unconjugated (bsm-33036M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33036m-100l

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bsm-33036m-bf350-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33036m-bf405-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-405

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: beta-Actin Antibody, conjugated (bsm-33036M-BF488) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: beta-Actin Antibody, conjugated (bsm-33036M-BF488) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: beta-Actin Antibody, conjugated (bsm-33036M-BF488) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: beta-Actin Antibody, conjugated (bsm-33036M-BF488) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33036m-bf488-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33036m-bf555-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-555

Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: beta-Actin Antibody, conjugated (bsm-33036M-BF594) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: beta-Actin Antibody, conjugated (bsm-33036M-BF594) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: beta-Actin Antibody, conjugated (bsm-33036M-BF594) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: beta-Actin Antibody, conjugated (bsm-33036M-BF594) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33036m-bf594-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33036m-bf647-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33036m-bf680-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33036m-bf750-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33036m-apc-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: APC

Catalog #1: bsm-33036m-apc-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: APC-Cy5.5

Catalog #1: bsm-33036m-apc-cy7-100l

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: APC-Cy7

Catalog #1: bsm-33036m-biotin-100l

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Biotin

Catalog #1: bsm-33036m-cy3-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Cy3

Catalog #1: bsm-33036m-cy5-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Cy5

Catalog #1: bsm-33036m-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Cy5.5

Catalog #1: bsm-33036m-cy7-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Cy7

Catalog #1: bsm-33036m-fitc-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody (bsm-33036M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

Catalog #1: bsm-33036m-hrp-100l

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: HRP

Catalog #1: bsm-33036m-pe-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: PE

Catalog #1: bsm-33036m-pe-cy5-100l

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: PE-Cy5

Catalog #1: bsm-33036m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: PE-Cy5.5

Catalog #1: bsm-33036m-pe-cy7-100l

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: PE-Cy7

Catalog #1: bsm-33036m-percp-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: PerCP

Catalog #1: bsm-33036m-percp-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33036m-rbitc-100l

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: RBITC

Rat lung lysate 30ug, probed (bs-0061R) at 1:20000 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

Catalog #1: bs-0061R-BF350

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-350

Catalog #1: bs-0061R-BF405

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-405

Catalog #1: bs-0061R-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-488

Catalog #1: bs-0061R-BF555

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-555

Catalog #1: bs-0061R-BF594

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-594

Catalog #1: bs-0061R-BF647

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-647

Catalog #1: bs-0061R-BF680

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-680

Catalog #1: bs-0061R-BF750

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-750

Catalog #1: bs-0061R-APC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC

Catalog #1: bs-0061R-APC-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC-Cy5.5