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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FTO) Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FTO Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FTO Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FTO Monoclonal Antibody, Unconjugated (bsm-60733R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60733r-TR

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60733r-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-60733r-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-60733r-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-60733r-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-60733r-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-60733r-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-60733r-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-60733r-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-60733r-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-60733r-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-60733r-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-60733r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-60733r-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-60733r-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-60733r-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-60733r-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-60733r-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-60733r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-60733r-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-60733r-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-60733r-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-60733r-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-60733r-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-60733r-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-60733r-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-60733r-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Lane 1: HeLa Cell lysates; Lane 2: U-251 MG Cell lysates; probed with FUBP3 (1216CT820.155.150) Monoclonal Antibody, unconjugated (bsm-51416M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells stained with FUBP3 (12C8) Monoclonal Antibody, bsm-51416M at 1:25 dilution, followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Cytoplasmic actin was counterstained with Alexa Fluor\u00ae 555 conjugated with Phalloidin (red).

Catalog #1: bsm-51416M

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51567m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Fumarase\/FH) Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Fumarase\/FH) Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Fumarase\/FH) Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Fumarase\/FH) Monoclonal Antibody, Unconjugated (bsm-33167M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33167m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33167M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33167M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-51522m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

The THP-1 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-FUT4 antibody (bsm-33111M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed THP-1 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (FUT4) monoclonal Antibody, unconjugated (bsm-33111M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FUT4 Monoclonal Antibody, Unconjugated (ascites of bsm-33111M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FUT4 Monoclonal Antibody, Unconjugated (ascites of bsm-33111M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33111m-100ul

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33111m-bf350-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33111m-bf405-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33111m-bf488-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33111m-bf555-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33111m-bf594-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33111m-bf647-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33111m-bf680-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33111m-bf750-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33111m-apc-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-33111m-apc-Cy5.5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-33111m-apc-cy7-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33111m-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33111m-cy3-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33111m-cy5-100ul

Applications: FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5