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Catalog #1: bs-0023M-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-0023M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-0023M-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-0023M-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0023M-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-51086M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

GST-Tagged fusion protein Overexpression E.coli Lysates probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GST-Tagged fusion protein Overexpression E.coli Lysates probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:2000~1:10000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GST-Tagged fusion protein probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:60000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GST tag was immunoprecipitated from GST-tagged mouse S100A7 expression vector whole cell lysate with bsm-33007M at 1\/150 dilution. Western blot was performed from the immunoprecipitate using protein A\/G beads. HRP conjugated Goat Anti-Mouse IgG (H&L) was used as secondary antibody.\r\n\r\nLane 1: GST-tagged mouse S100A7 expression vector whole cell lysate 5 \u00b5g (Input).\r\nLane 2: bsm-33007M IP in GST-tagged mouse S100A7 expression vector whole cell lysate.\r\nLane 3: native mouse IgG IP in GST-tagged mouse S100A7 expression vector whole cell lysate (negative control).\r\n

Catalog #1: bsm-33007m-100ul

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33007M-BF350

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33007M-BF405

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33007M-BF488

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33007M-BF555

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33007M-BF594

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33007M-BF647

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33007M-BF680

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33007M-BF750

Applications: WB

Reactivity: Species independent

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33007M-APC

Applications: WB

Reactivity: Species independent

Conjugate: APC

Catalog #1: bsm-33007M-APC-Cy5.5

Applications: WB

Reactivity: Species independent

Conjugate: APC-Cy5.5

Catalog #1: bsm-33007M-APC-Cy7

Applications: WB

Reactivity: Species independent

Conjugate: APC-Cy7

Catalog #1: bsm-33007M-Biotin

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-33007M-Cy3

Applications: WB

Reactivity: Species independent

Conjugate: Cy3

Catalog #1: bsm-33007M-Cy5

Applications: WB

Reactivity: Species independent

Conjugate: Cy5

Catalog #1: bsm-33007M-Cy5.5

Applications: WB

Reactivity: Species independent

Conjugate: Cy5.5

Catalog #1: bsm-33007M-Cy7

Applications: WB

Reactivity: Species independent

Conjugate: Cy7

Catalog #1: bsm-33007M-FITC

Applications: WB

Reactivity: Species independent

Conjugate: FITC

Catalog #1: bsm-33007M-HRP

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-33007M-PE

Applications: WB

Reactivity: Species independent

Conjugate: PE

Catalog #1: bsm-33007M-PE-Cy5

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy5

Catalog #1: bsm-33007M-PE-Cy5.5

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy5.5

Catalog #1: bsm-33007M-PE-Cy7

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy7

Catalog #1: bsm-33007M-PerCP

Applications: WB

Reactivity: Species independent

Conjugate: PerCP

Catalog #1: bsm-33007M-PerCP-Cy5.5

Applications: WB

Reactivity: Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33007m-percp-cy7-100ul

Applications: WB

Reactivity: Species independent

Conjugate: PerCP-Cy7

Catalog #1: bsm-33007M-RBITC

Applications: WB

Reactivity: Species independent

Conjugate: RBITC

Catalog #1: bsm-51083M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51742m

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51008M

Applications: WB

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bsm-51225M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293 Cell lysates; Lane 2: CEM Cell lysates; Lane 3: HepG2 Cell lysates; Lane 4: Jurkat Cell lysates; Lane 5: HeLa Cell lysates; Lane 6: Raji Cell lysates; probed with H2AFX (8C5) Monoclonal Antibody, unconjugated (bsm-51222M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Thymus tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody (bsm-51222M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human prostate tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody (bsm-51222M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with H2AFX (8C5) Monoclonal Antibody, Unconjugated (bsm-51222M) at 1:50 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-51222M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51023R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-53023M

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

HeLa cells were stained with H3K27ac (1A3) Monoclonal Antibody (bsm-53022M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27ac antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two staining is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K27ac (1A3) Monoclonal Antibody (bsm-53022M). The wells were coated with peptides containing the unmodified H3K27 region as well as the acetylated H3K27 and the acetylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.
ChIP assays were performed using HeLa cells, H3K27ac (1A3) Monoclonal Antibody (bsm-53022M) and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 \u00b5g of antibody per ChIP experiment was analyzed. IgG (1\u00b5g\/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the EIF4A2 and GAPDH genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53022M

Applications: WB, ELISA, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51021R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-53009M

Applications: WB, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using H3K27me3 (9B7) Monoclonal Antibody (bsm-53019M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H3K27me3 (9B7) Monoclonal Antibody (bsm-53019M) and DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse secondary antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K27me3 (9B7) Monoclonal Antibody (bsm-53019M). The wells were coated with peptides containing the unmodified H3K27 region as well as the mono-, di- and trimethylated H3K27 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.
ChIP assays were performed using HeLa cells, H3K27me3 (9B7) Monoclonal Antibody (bsm-53019M) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 \u03bcg per ChIP experiment was analysed. IgG (2 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH as negative controls, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3 is preferably present at inactive genes.

Catalog #1: bsm-53019M

Applications: WB, ELISA, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51019R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

To test the specificity an ELISA was performed using a serial dilution using 3K36me2 (6C9) Monoclonal Antibody (bsm-53020M). The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.
ChIP assays were performed using HeLa cells, H3K36me2 (6C9) Monoclonal Antibody (bsm-53020M) and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 \u03bcg of antibody per ChIP experiment was analyzed. IgG (1 \u03bcg\/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a genomic region upstream of the TGM2 gene, used as a positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53020M

Applications: ELISA, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-51020R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

HeLa cells were stained with H3K36me3 (11D1) Monoclonal Antibody (bsm-53021M) and DAPI. Cells were fixed with methanol and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K36me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K36me3 (11D1) Monoclonal Antibody (bsm-53021M). The wells were coated with peptides containing unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.
ChIP assays were performed using HeLa cells, H3K36me3 (11D1) Monoclonal Antibody (bsm-53021M) and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 \u03bcg of antibody per ChIP experiment was analyzed. IgG (1 \u03bcg\/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding regions of the active EIF2S3 and CCT5 genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53021M

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated