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Catalog #1: bsm-33188m-cy7-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33187m-fitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33188m-fitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33187m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33188m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33187m-pe-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33188m-pe-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33187m-pe-cy5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-33188m-pe-cy5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-33187m-pe-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33188m-pe-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-33187m-pe-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33188m-pe-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-33187m-percp-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33188m-percp-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-33187m-percp-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33188m-percp-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33188m-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-33187m-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-33187m-rbitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-33188m-rbitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-70013M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
RSC96 probed with alpha Tubulin (4G1) Monoclonal Antibody, Unconjugated (bsm-50179M) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human cervical carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Hela; Lane 2: 293T; Lane 3: MDA-MB-231; Lane 4: A549; Lane 5: A431; Lane 6: RSC96; Lane 7: VSC4.1; Lane 8: ASPC-1; Lane 9: B16; Lane 10: BV2; Lane 11: H9C2; Lane 12: Hep2G;Lane 13: K562; Lane 14: U937; probed with alpha Tubulin (4G1) Monoclonal Antibody, unconjugated (bsm-50179M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.\r\n

Catalog #1: bsm-50179M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, All

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with alpha Tubulin (Acetyl Lys40) monoclonal antibody, unconjugated (bsm-33235M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
C6 (R) cells were treated with or without Trichostatin A (500 ng\/ml) for 4 h, Hela (H) cells were treated with or without Trichostatin A (500 ng\/ml) for 4 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with alpha Tubulin (Acetyl Lys40) monoclonal antibody, unconjugated (bsm-33235M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33235m-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #1: bsm-33235m-bf350-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33235m-bf405-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33235m-bf488-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33235m-bf555-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33235m-bf594-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33235m-bf647-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33235m-bf680-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33235m-bf750-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33235m-apc-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: APC

Catalog #1: bsm-33235m-apc-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: APC-Cy5.5

Catalog #1: bsm-33235m-apc-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: APC-Cy7

Catalog #1: bsm-33235m-biotin-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Biotin

Catalog #1: bsm-33235m-cy3-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Cy3

Catalog #1: bsm-33235m-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Cy5

Catalog #1: bsm-33235m-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Cy5.5

Catalog #1: bsm-33235m-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Cy7

Catalog #1: bsm-33235m-fitc-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: FITC

Catalog #1: bsm-33235m-hrp-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: HRP

Catalog #1: bsm-33235m-pe-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PE

Catalog #1: bsm-33235m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PE-Cy5

Catalog #1: bsm-33235m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PE-Cy5.5

Catalog #1: bsm-33235m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PE-Cy7

Catalog #1: bsm-33235m-percp-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PerCP

Catalog #1: bsm-33235m-percp-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PerCP-Cy5.5